Alister Burt
@alisterburt.bsky.social
370 followers 390 following 210 posts
Computational Cryo-EM/ET at Genentech Love people, the outdoors and building stuff Complexity is the enemy All opinions my own
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Reposted by Alister Burt
alexisrohou.bsky.social
An insightful read. I hadn’t appreciated the links between feudalism and privatization. The author’s evocation of how future historians may look back on our era in that respect felt important, and a fruitful way to think, to me. I’m hopeful the pendulum will swing back towards effective government.
Feudalism Is Our Future
What the next Dark Ages could look like
www.theatlantic.com
alisterburt.bsky.social
Thanks for sharing Alexis, reading now - huge +1 on Tom’s recommendation, Varoufakis is also excellent in podcast form

youtu.be/VatYrw0uqjU?...
American Big Tech Has Enslaved Us | Aaron Bastani Meets Yanis Varoufakis
YouTube video by Novara Media
youtu.be
alisterburt.bsky.social
We’ll have to compare, think mine is from the 70s
alisterburt.bsky.social
I’ll save your wallet by drip feeding recommendations going forwards 😂
alisterburt.bsky.social
Don Fawcett’s big red book The Cell is available for free online!!

There are a bajillion cryo-ET projects in there, recommend a physical copy if you can find one - it’s my favourite coffee table book
olibclarke.bsky.social
And it's available to borrow/view at archive.org, nice!

archive.org/details/cell...
alisterburt.bsky.social
Nice find!! Glad there’s a free digital copy out there - still recommend a physical copy if you can, I don’t have a coffee table but it’s my favourite coffee table book
alisterburt.bsky.social
A small group of us bought the few used copies available on Amazon UK a few years ago, got to watch supply and demand do its thing live - hope you can find one for a good price!
alisterburt.bsky.social
don’t have an answer for you but if you’re looking at cell tomograms often I can’t recommend Don Fawcett’s big red book The Cell highly enough - it’s a *beautiful* atlas of classic EM images
alisterburt.bsky.social
Oooh no, will take a look!
alisterburt.bsky.social
This is one thing the ML folks are doing right, the blog post + arxiv combo is all you (should) need, wish it was more normalised for bio too
pengzell.bsky.social
In academia, the pinnacle of achievement is a pdf no one can access and I think that's beautiful
alisterburt.bsky.social
Right! Anything powered by the source engine brings back such great memories 🥰
alisterburt.bsky.social
the GNE headcrab went for its latest victim today, a wild @sjorsscheres.bsky.social

Thankfully @przemekdut.bsky.social was there to save the day!
Reposted by Alister Burt
llorentealejandro.bsky.social
1/9 🚀 Big news! As a result of 5 years of my PhD, building on a 30-year dream of my PI Dr. Nancy Carrasco, we think we've found a new way to treat cancer! We’ve released our preprint & I'm so excited to share this story! 🧵
www.biorxiv.org/content/10.1...
#Science #proteinengineering #proteindesing
Engineering substrate selectivity in the human sodium/iodide symporter (NIS)
Iodide (I-) uptake mediated by the Na+/I- symporter (NIS) is the first step in the biosynthesis of the thyroid hormones, of which I- is an essential constituent. NIS couples the inward transport of I-...
www.biorxiv.org
alisterburt.bsky.social
so satisfying - question: are all the best microscopists Dutch?
alisterburt.bsky.social
This has been a real pain point for Warp users since we moved to Linux, psyched to see it solved

Awesome work @hamid13r.bsky.social ! Thank you for taking the time to share your solution more widely :-)
hamid13r.bsky.social
Working on #WarpTools I have the old problem of removing "bad tilts". These are views that I skipped in #etomo to get a better model...

I wrote a python code to turn the UseTilt to False in the XML files for those views and improve the tomo reconstruction.
#teamtomo
github.com/hamid13r/war...
warp_lamella_adapters/remove_bad_views.py at main · hamid13r/warp_lamella_adapters
A short optimized pipeline to get lamella tomography (with pretilt and with some tilts obscured or mistracked) to work well with Warp - hamid13r/warp_lamella_adapters
github.com
alisterburt.bsky.social
finally hit πthon
savannah.dev
The stable release of Python 3.14 is out now! Go, go, go update! 🙌

discuss.python.org/t/python-3-1...
Cute illustrated logo featuring a pink/peach colored pie displaying ‘3.14’ (pi), surrounded by two snakes in blue and yellow. The design is encircled by text reading ‘r-strings • zsid • free-threading • support • sub-interpreters • REPL highlighting • note colour • colour’ in a circular arrangement. The illustration uses a soft pastel color palette with blue, yellow, and pink tones, and includes decorative sparkle elements.
Reposted by Alister Burt
meiercredo.bsky.social
Check out our new preprint on an integrated pipeline combining in situ #cryo-ET with MALDI #MSImaging for single-cell identification and classification from previously analysed EM-grids.
Link: www.biorxiv.org/content/10.1...
Reposted by Alister Burt
jhschaef.bsky.social
Helical Reconstruction of Amyloids in cryoSPARC?
We present guidelines, limitations & future perspectives for processing amyloids in cryoSPARC @structurabio.bsky.social.
Great collaboration of the Kelly Lab and @landerlab.bsky.social #cryoEM #amyloid www.biorxiv.org/content/10.1...
Amyloid reconstructions from cryoSPARC
Reposted by Alister Burt
astrokatie.com
I think for me the most compelling answer for "why fund basic research?" (and the one most relevant to the people doing the work) is that humans are curious and finding stuff out makes us happy and fulfilled. Science is a thing humans like. Life would be more dull and sad if we didn't do it.

end/🧵