Organised by

@andreas-t-schaefer.bsky.social, @em-stp.bsky.social, @carlesbosch.bsky.social, @safekhan.bsky.social, @jkbrmn.bsky.social, @yuxinzhang.bsky.social & Anika Tailor

Register here:
tinyurl.com/crickxrays25

Join us *next Monday*!
#CrickXrays25
X-ray #nanoimaging of biological tissues

🇬🇧🌐 @crick.ac.uk & online
tinyurl.com/crickxrays25

Sponsored by @dectris.bsky.social, @webknossos.org and @biologists.bsky.social and backed by @brukercorporation.bsky.social and Histomography

#CrickXrays25 is organised by @andreas-t-schaefer.bsky.social, @em-stp.bsky.social, @carlesbosch.bsky.social, @safekhan.bsky.social, @jkbrmn.bsky.social & @yuxinzhang.bsky.social with the assistance of Anika Tailor.

Hard X-ray imaging of biological soft tissues.
tinyurl.com/crickxrays25

Join us!

With invited speakers from @esrf.fr, Northwestern U, ALBA, [email protected], @psich.bsky.social, Argonne National Lab, @uni-goettingen.de, TU Denmark, @yaleschoolofmed.bsky.social, @crick.ac.uk, @ucl.ac.uk and from industry partners @dectris.bsky.social and @webknossos.org.

tinyurl.com/crickxrays25

#Nanoimaging symposium @crick.ac.uk!

At #CrickXrays25 we'll tackle:
💫synchrotron upgrades
📈current limits in throughput, volume, resolution
🧪sample preparation for X-ray PC imaging
🧠♥️applications in neuroscience and tissue life science

🗓️13th October 2025
🇬🇧🌐 London & online
tinyurl.com/crickxrays25

Kudos to all authors involved and thanks to all the institutions that have hosted and supported this research, including the #synchrotrons DESY and @esrf.fr.

@crick.ac.uk @embl.org @imperialcollegeldn.bsky.social @uclnpp.bsky.social

#preprint:

We optimised the preparation of tissues for #X-ray phase contrast imaging.

Drying tissue samples (instead of embedding them) brings more signal in #X-ray phase contrast images.

The process largely preserves #ultrastructure.

@safekhan.bsky.social @andreas-t-schaefer.bsky.social

Wouldn't it be great if we could not only image large connectomic volumes but also completely reconstruct them? And if a whole mouse brain project didn't cost billions?

With the PATHFINDER preprint (www.biorxiv.org/content/10.1...), we preview a future where it doesn't have to.

And to the whole team: Alfred Laugros, Jayde Livingstone, Manuel Berning, Sina Tootoonian, Mihaly Kollo, Andrea Nathansen and @normanrz.com

@crick.ac.uk @uclnpp.bsky.social @esrf.fr @psich.bsky.social @zeiss-microscopy.bsky.social @webknossos.org @mpibrain.bsky.social

Enjoy the read!

Cheers to @tobiasackels.bsky.social, with whom we started the correlative workflow, to Anne Bonnin, key in the SXRT datasets that articulate the pipeline, and to Carsten Waltenberg for milling a targeted cylinder containing the neuronal circuits.

@psich.bsky.social @zeiss-microscopy.bsky.social

Big shoutout to all collaborators. Starting with @yuxinzhang.bsky.social, whose PhD evolved building this story. To @andreas-t-schaefer.bsky.social, core PI in this endeavour, and @apacureanu.bsky.social and Peter Cloetens for the expert insight on X-ray holotomography.

@crick.ac.uk @esrf.fr

We developed our correlative multimodal imaging pipeline to incorporate Synchrotron X-ray Holographic Nanotomography.
With it we mapped a targeted 0.3mm^3 volumes with subcellular detail, non-destructively.

With sufficient reliability to gather 3 replicates of the same neuronal circuit.

In a nutshell, neurons of the same olfactory circuit in the mammalian brain share firing properties while showing individual traits.

Check out the tweetorial below by @yuxinzhang.bsky.social for the main findings:

bsky.app/profile/yuxi...

#preprint:

We mapped the structure and function of olfactory bulb circuits with in vivo 2-photon microscopy and synchrotron #Xray holographic nanotomography.

doi.org/10.1101/2025...

@yuxinzhang.bsky.social @andreas-t-schaefer.bsky.social @apacureanu.bsky.social
@crick.ac.uk @esrf.fr

Folks! We need your help to establish this platform long term for everyone! Can you help us out spread this video?

That’s amazing, congrats everyone!!