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The Groth lab @grothlab.bsky.social · Aug 28

It was wonderful to hear talks from EpiC members @grothlab.bsky.social @nilskrietenstein.bsky.social @nilssonlab.bsky.social & Niels Mailand, now we are settled in our new home @cancer.dk. We are grateful for @dg.dk for this exciting opportunity. Onwards and upwards with EpiC science! 🧬 #CPRLegacy

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The Groth lab @grothlab.bsky.social · Aug 28

We have lift off!! 🚀 Yesterday was the official opening of EpiC! The DNRF Center for Epigenetic Cell Memory at the Danish Cancer Institute!
Our EpiC team with director Anja Groth @groth-anja.bsky.social, were joined by Jesper Fisker, CEO @cancer.dk & Niels Mejlgaar, CEO @dg.dk to celebrate 🎉

Image of Niels Mejlgaar (CEO of DNRF), Anja Groth (EpiC director) & Jesper Fisker (CEO of DCS).

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Reposted by The Groth lab

Alessandro Costa @costalaboratory.bsky.social · Jul 21

Less than two weeks left to apply!

Postdoc positions in my lab to study

1. initiation of DNA replication.

2. chromatin replication/epigenetic inheritance.

Great for biochemists, biophysicists & structural biologists.

Deadline 3 August 2025.

crick.wd3.myworkdayjobs.com/External/job...

Postdoctoral Fellow - Costa Lab

Salary for this Role: From £45,500 with benefits, subject to skills and experience. Job Title: Postdoctoral Fellow - Costa Lab Reports to: Alessandro Costa Closing Date: 03/Aug/2025 23.59 GMT Job Desc...

crick.wd3.myworkdayjobs.com

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The Groth lab @grothlab.bsky.social · Jun 27

Congratulations to Ada 🥳🍾, who very successfully defended her master's thesis today! You were an excellent student and we were super happy to have you in the lab! Wishing you all the best for the future and enjoy your well-deserved break!

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Reposted by The Groth lab

Alessandro Costa @costalaboratory.bsky.social · Jun 20

Postdoc positions open in my lab (Francis Crick Institute) to study

1. initiation of DNA replication.

2. chromatin replication/epigenetic inheritance.

Great for biochemists, biophysicists and cryo-EM/cryo-tomography scientists.

Deadline 3 August 2025.

crick.wd3.myworkdayjobs.com/External/job...

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The Groth lab @grothlab.bsky.social · May 20

🚨 Postdoc wanted (🇳🇱NL based) for our exciting collaboration with the lab of Alexander van Oudenaarden @hubrechtinstitute.bsky.social in Utrecht, with collaborative visits to our lab Copenhagen 🇩🇰. We seek a computational scientist to model epigenetic inheritance, see more below 👇!

Jeroen van den Berg @jervdberg.bsky.social · May 16

🧬Postdoc position alert! 🚨 We're hiring a postoc in Computational Modeling of Epigenetic Inheritance at the Hubrecht Institute (Alexander van Oudenaarden, Utrecht 🇳🇱) & Danish Cancer Institute (Anja Groth, Copenhagen 🇩🇰).

Come work in our collaberative team of top scientists

shorturl.at/avBBy

Postdoctoral Researcher - Computational modeling of Epigenetic Inheritance - 38 hours p/w - Hubrecht Institute

About the Project Faithful inheritance of the epigenome in proliferating cells critically underpins human development and health. While DNA replication fidelity and DNA mutation rates are well underst...

shorturl.at

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The Groth lab @grothlab.bsky.social · May 1

Welcome Marcel!!

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The Groth lab @grothlab.bsky.social · Mar 10

Thank you, Tae-Kyeong!

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The Groth lab @grothlab.bsky.social · Mar 6

Together our work gives a molecular basis for histone supply regulation by CODANIN-1, read the full paper here: rdcu.be/ecbHq . Thanks to the Song lab at KAIST jijoonsong.bsky.social for taking us onboard as collaborators for this exciting new structure-function adventure!

CODANIN-1 sequesters ASF1 by using a histone H3 mimic helix to regulate the histone supply

Nature Communications - CODANIN-1 negatively regulates the function of ASF1, the key chaperone for histone H3-H4 supply. Here the authors present the cryo-EM structure of the CODANIN-1_ASF1A...

rdcu.be

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The Groth lab @grothlab.bsky.social · Mar 6

How important is CODANIN/ASF1A complex formation? Inhibiting dimerization in cells or introducing a pathogenic CDA-I mutation in the dimerization interface reduced ASF1A cytoplasmic sequestration. This shows the significance of CODANIN/ASF1A complex formation and possible disease relevance

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The Groth lab @grothlab.bsky.social · Mar 6

Which CODANIN binding sites are required for ASF1 regulation? Using inducible complementation of CODANIN-1 in cells, we found that mutation of both BD-N and HMH-C is needed to prevent cytoplasmic sequestration of ASF1A. Thus, CODANIN-1 regulates ASF1A nuclear import by this dual-binding mechanism.

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The Groth lab @grothlab.bsky.social · Mar 6

By binding in this way, CODANIN-1 occludes the two major histone-binding surfaces of ASF1A, preventing its chaperone activity. Interestingly, CODANIN-1 could also bind preformed ASF1A/H3-H4 using its B domains, suggesting that it may regulate ASF1 at multiple points in the histone supply chain.

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The Groth lab @grothlab.bsky.social · Mar 6

So, how does CODANIN-1 bind ASF1? Our cryo-EM structure reveals that CODANIN-1 forms a dimer, with each monomer binding two ASF1A molecules. These two ASF1As are held in proximal and distal positions by CODANIN’s N-terminal B domain (BD-N) and C-terminal histone H3 mimic helix (HMH-C), respectively.

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The Groth lab @grothlab.bsky.social · Mar 6

A bit of background: ASF1 plays a central role in histone nuclear import and supply for replication & transcription. CODANIN-1 is commonly mutated in a rare form of heritable anaemia (CDA-I) and inhibits histone supply by ASF1. But we don’t currently understand these roles on the molecular level.

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The Groth lab @grothlab.bsky.social · Mar 6

𝐍𝐞𝐰 𝐦𝐞𝐜𝐡𝐚𝐧𝐢𝐬𝐭𝐢𝐜 𝐢𝐧𝐬𝐢𝐠𝐡𝐭𝐬 𝐢𝐧𝐭𝐨 𝐭𝐡𝐞 𝐫𝐞𝐠𝐮𝐥𝐚𝐭𝐢𝐨𝐧 𝐨𝐟 𝐡𝐢𝐬𝐭𝐨𝐧𝐞 𝐬𝐮𝐩𝐩𝐥𝐲! Excited to share our latest collaboration led by tkjeong.bsky.social and ciaranfrater.bsky.social! Structural determination of the histone chaperone ASF1 and its regulator CODANIN-1 uncovers how CODANIN-1 counters ASF1 activity. 🧵👇

CODANIN-1 sequesters ASF1 by using a histone H3 mimic helix to regulate the histone supply

Nature Communications - CODANIN-1 negatively regulates the function of ASF1, the key chaperone for histone H3-H4 supply. Here the authors present the cryo-EM structure of the CODANIN-1_ASF1A...

rdcu.be

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Reposted by The Groth lab

Anja Groth @groth-anja.bsky.social · Feb 26

SAVE THE DATE! The next EMBO | EMBL Symposium on 'DNA replication and genome maintenance: from basic biology to disease' will be held October 20-23, 2026.

Organized by Helle Ulrich (IMB), Johannes Walter (Harvard) and Anja Groth (Danish Cancer Institute).

www.embl.org/about/info/c...

DNA replication and genome maintenance: from basic biology to disease

www.embl.org

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Reposted by The Groth lab

Robin Allshire 🇮🇪 🇪🇺✊🏿 ⬆️ Zak’s favourite nucleosome: K9me @allshire-lab.bsky.social · Feb 25

Epigenetics Gordon Research Conference. Consider joining us for what will be an excellent, exciting meeting and will be held in Europe for the first time: 10-15 Aug25 Barcelona, Spain. Please repost and apply early - this meeting is popular! www.grc.org/epigenetics-...

2025 Epigenetics Conference GRC

The 2025 Gordon Research Conference on Epigenetics will be held in Castelldefels, Barcelona Spain. Apply today to reserve your spot.

www.grc.org

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Reposted by The Groth lab

Marcel Werner @marcelwerner.bsky.social · Feb 20

I am delighted to share the main work of my PhD project in the @hamperllab.bsky.social now out in @narjournal.bsky.social. TRCs drive R-loop-dependent nucleosome eviction and require DOT1L activity for transcription recovery.
academic.oup.com/nar/article/...

#TRC #r-loop #chromatin

More below 👇

Transcription-replication conflicts drive R-loop-dependent nucleosome eviction and require DOT1L activity for transcription recovery

Abstract. Progressing transcription and replication machineries profoundly impact their underlying chromatin template. Consequently, transcription-replicat

academic.oup.com

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The Groth lab @grothlab.bsky.social · Feb 20

Thank you, Jeroen!

The Groth lab @grothlab.bsky.social · Feb 19

If you’re interested in epigenetic inheritance and cell division, check out the vacancies for 6 PhDs and Postdocs positions in our new Center for Epigenetic Cell Memory (EpiC): tinyurl.com/yvd93na2 6/n

6 Postdoctoral and PhD positions in Center for Epigenetic Cell Memory (EpiC)

The Center for Epigenetic Cell Memory (EpiC) is a new Center of Excellence that will be established based on funding by the Danish National Research Foundation.

tinyurl.com

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The Groth lab @grothlab.bsky.social · Feb 19

Want to dive deeper? 🧐 Read the full paper here: tinyurl.com/233y89d2 🔗📖. Last, a big thank you to all our collaborators and colleagues at #NNF-CPR 🙏. #HistoneRecycling #Epigenetics #Chromatin #ScienceAdvances 5/n

Disabling leading and lagging strand histone transmission results in parental histones loss and reduced cell plasticity and viability

Losing parental histones during DNA replication fork passage challenges differentiation competence and cell viability.

tinyurl.com

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The Groth lab @grothlab.bsky.social · Feb 19

Our work highlights 𝐩𝐚𝐫𝐞𝐧𝐭𝐚𝐥 𝐡𝐢𝐬𝐭𝐨𝐧𝐞 𝐫𝐞𝐜𝐲𝐜𝐥𝐢𝐧𝐠 𝐚𝐬 𝐚 𝐤𝐞𝐲 𝐞𝐩𝐢𝐠𝐞𝐧𝐞𝐭𝐢𝐜 𝐬𝐚𝐟𝐞𝐠𝐮𝐚𝐫𝐝 – histone modifications must be 𝐞𝐟𝐟𝐢𝐜𝐢𝐞𝐧𝐭𝐥𝐲 transmitted to 𝐛𝐨𝐭𝐡 daughter strands for this to work! Could failures in this process contribute to developmental disorders, cancer, or aging? More to explore! 4/n

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The Groth lab @grothlab.bsky.social · Feb 19

What happens next? Gene misregulation & differentiation defects start to emerge. But when 𝐛𝐨𝐭𝐡 leading & lagging strand recycling are disrupted (POLE4 & MCM2-2A), 𝐬𝐨𝐦𝐞 𝐩𝐚𝐫𝐞𝐧𝐭𝐚𝐥 𝐡𝐢𝐬𝐭𝐨𝐧𝐞𝐬 𝐚𝐫𝐞 𝐥𝐨𝐬𝐭 𝐢𝐧 𝐭𝐫𝐚𝐧𝐬𝐦𝐢𝐬𝐬𝐢𝐨𝐧 —worsening the defects. Unlike single mutants, cells now start to die! 3/n

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The Groth lab @grothlab.bsky.social · Feb 19

We use a degron to deplete POLE4, a leading strand recycler, in WT or a background defective in lagging strand recycling (MCM2-2A). The first epigenome alteration upon acute loss of leading strand recycling is 𝐚𝐜𝐜𝐮𝐦𝐮𝐥𝐚𝐭𝐢𝐨𝐧 𝐨𝐟 𝐇𝟑𝐊𝟐𝟕𝐦𝐞𝟑 - 𝐰𝐢𝐭𝐡𝐢𝐧 𝟏-𝟐 𝐜𝐞𝐥𝐥 𝐜𝐲𝐜𝐥𝐞𝐬 - 𝐛𝐞𝐟𝐨𝐫𝐞 𝐭𝐫𝐚𝐧𝐬𝐜𝐫𝐢𝐩𝐭𝐢𝐨𝐧𝐚𝐥 𝐜𝐡𝐚𝐧𝐠𝐞𝐬! 2/n

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The Groth lab @grothlab.bsky.social · Feb 19

During replication, modified parental histones are transmitted to the daughter DNA strands - they are recycled. Recycled histones serve as agents of memory to maintain the epigenome in daughter cells! What happens when recycling is acutely disrupted? 1/n

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