The hits keep on coming from @parallelsq.bsky.social! Today we released an exciting use-case for plexDIA in single cell analysis of #alzheimer's disease, characterizing dysregulation of the 20S proteasome. #TeamMassSpec #Proteomics

www.biorxiv.org/content/10.1...

Members of PTI have developed mass tags which improve spectral quality and enable 9-plexDIA in a project lead by @harrisons.bsky.social

Links to our preprints:

timePlex (multiplexing with time-offsets): www.biorxiv.org/content/10.1...

JMod (search software for joint-modeling): www.biorxiv.org/content/10.1...

PSMtag (novel mass tag for 9-plexDIA): www.biorxiv.org/content/10.1...

This work was a collective effort by members of @parallelsq.bsky.social, enabled analytically through the development of specialized search software (JMod) and through mass-tag engineering (PSMtag).

We paired 3-timePlex with @parallelsq.bsky.social's newly developed mass tag (PSMtag) used for 9-plexDIA.

This enables 27-plex, and we achieve >500 SPD with 25 minutes active chromatography per sample!

How have we implemented timePlex?

We stagger and overlap the chromatography of multiple samples in the same run. The time-offsets between samples encode sample-specificity.

The time-offsets in timePlex encode samples analogously to mass-offsets in plexDIA.

Time and mass dimensions are orthogonal, which unlocks combinatorial multiplexing when timePlex and plexDIA are used together! This introduces the potential for quadratically increased throughput!

We are excited to introduce ‘time’ as a new domain for proteomics multiplexing!

It enables:
-Label-free multiplexing
-Combinatorial multiplexing with plexDIA

Using combined 9-plexDIA and 3-timePlex we demonstrate 27-plex DIA 🚀