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jovanakaljevic.bsky.social
Jovana Kaljevic ⬜️
@jovanakaljevic.bsky.social
31 followers 15 following 4 posts
Postdoctoral scientist | JIC, UK PhD | de Duve institute, Belgium Passionate about killing E. coli and watching them die under the🔬
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jovanakaljevic.bsky.social
Jovana Kaljevic ⬜️ @jovanakaljevic.bsky.social · 1d
Thrilled to see this work out. I enjoyed our collaboration @antoinehocher and loved working on this project. Congrats to the entire team! @tunglejic.bsky.social
tunglejic.bsky.social
Tung Le @tunglejic.bsky.social · 1d
new preprint from our group & Antoine Hocher: www.biorxiv.org/content/10.1...
A fantastic collaboration with Antoine, with Jovana Kaljevic' initiated the collaboration and drives the project.
Versatile NTP recognition and domain fusions expand the functional repertoire of the ParB-CTPase fold beyond chromosome segregation
Nucleotide triphosphate (NTP)-dependent molecular switches regulate essential cellular processes by cycling between active and inactive states through nucleotide binding and hydrolysis. These mechanis...
www.biorxiv.org
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Reposted by Jovana Kaljevic ⬜️
tunglejic.bsky.social
Tung Le @tunglejic.bsky.social · 1d
ParB NTPase fold might:
*suggest hidden layer of biological regulation
*maybe a fold as fundamental as P-loop ATPases or Rossmann folds (well, this has to be seen!)
*be a treasure trove of uncharacterized proteins waiting to be studied.

We probably have only scratched the surface.
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jovanakaljevic.bsky.social
Jovana Kaljevic ⬜️ @jovanakaljevic.bsky.social · 6d
This was fun!
lalouxlab.bsky.social
Géraldine Laloux @lalouxlab.bsky.social · 26d
🚨New paper out! #MicroSky
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
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Expanding the genetic toolbox of the obligate predatory bacterium Bdellovibrio bacteriovorus with inducible gene expression and CRISPR interference
Inducible gene expression and CRISPR interference-mediated depletion in the predatory bacterium Bdellovibrio bacteriovorus.
academic.oup.com
Reposted by Jovana Kaljevic ⬜️
lalouxlab.bsky.social
Géraldine Laloux @lalouxlab.bsky.social · 18d
📝New preprint! #MicroSky

🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.

🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.

www.biorxiv.org/content/10.1...
Dynamics and interplay of gene expression and chromosome organization across a predatory lifecycle
The obligate predatory bacterium Bdellovibrio bacteriovorus alternates between a motile attack phase and a growth phase inside another bacterium, during which it undergoes multiple rounds of DNA repli...
www.biorxiv.org
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jovanakaljevic.bsky.social
Jovana Kaljevic ⬜️ @jovanakaljevic.bsky.social · Nov 28
doi.org/10.1038/d41586…

Check out our story! From inseparable undergrads in Belgrade to PhDs scattered across the globe, FFDS (Four Friends Doing Science) kept us inspiring each other, no matter the distance.

Can you guess who’s who😆?
@BorisStojilkovic @milicajelicic
@tismaa.bsky.social
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jovanakaljevic.bsky.social
Jovana Kaljevic ⬜️ @jovanakaljevic.bsky.social · Nov 28
I enjoyed so much writing this piece! Read the story of four friends doing science!
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