Comprehensive high-resolution mass spectrometry analysis of Brazilian bracatinga (Mimosa scabrella Bentham) honeydew honeys and floral honeys from Bra…

Astringency in red wine is primarily caused by condensed tannins interaction with salivary proteins, thus contributing to wine mouthfeel. Condensed ta…

In this study, the stability of chlorogenic acids during long-term coffee storage was investigated. Two time series including green coffee beans over …

Edible insects are nutritious, having high levels of protein, essential amino acids, healthy fats, vitamins, and minerals. In areas where sorghum is a primary crop, mixing this gluten-free grain with....

Translational Psychiatry - Discovery of molecular signature of long-term psychiatric sequelae in COVID-19 through proteome profiling of dried blood spots

Glyphosate (GLY) is the most widely used pesticide in the world. Agricultural workers are exposed to GLY via ingestion and skin contact. GLY may generate epigenetic changes that might be carcinogenic. However, whether GLY is carcinogenic remains controversial. We investigated whether GLY was co-carcinogenic with ultraviolet radiation (UVR) by monitoring skin tumor development in hairless mice. Four groups of immunocompetent mice (total, N = 96) were used in the study. Two groups received GLY in drinking water (5 µg/mL or 50 µg/mL), one group received 50 µL topical GLY (6.59% w/w) three times per week, and a UVR control group received only tap water. All groups were given UVR until they developed three 4-mm tumors or one 12-mm tumor. Times to development of first, second, and third skin tumors were recorded. In both groups given GLY orally (medians, 163 days) and the group given GLY topically (median, 170 days), the times to skin tumor development were not significantly different from the control group (median, 163 days; p ≥ 0.535). Mass spectrometry imaging showed that topically applied GLY was present on the surface of the skin but not detectable within the skin. There were no differences in weight, erythema, or pigmentation. When combined with UVR, GLY in drinking water or directly applied to skin was not associated with increased skin tumor development. Graphical abstract fx1

Sedimentary organic matter (SOM), an organic mixture of complex compositions with diverse structures, plays a crucial role in lake carbon cycling. The…

Leishmania donovani is the causative agent of visceral leishmaniasis, a tropical disease affecting millions worldwide. While proteomic studies of Leis…

Skeletal muscle is the main consumer of glucose after a mixed meal, and resistance exercise further increases muscle glucose uptake. Emerging evidence suggests that glucose uptake in muscles is not on...

The European Journal of Translational Myology focuses on basic, applied, and translational research in myology, muscle diseases, and rehabilitation.

Catalytic ozonation is a widely used advanced oxidation process for treating refractory organic wastewater; yet, the variability in dissolved organic matter (DOM) composition complicates reaction mech...

Background Sebaceous glands (SGs) secrete sebum to form a protective barrier that maintains skin health. However, exposure to ultraviolet A (UV-A) radiation damages the skin barrier, leading to dryn...

Plant glandular trichomes (GTs) are characterised by their ability to synthesise and store abundant secondary metabolites of significant biological importance. The medicinal plant Artemisia argyi exh....

Tetrahydrocannabinol (THC), the primary psychoactive compound of cannabis, is the most widely abused substance worldwide, with an annual prevalence of 4.3% of adults and 5.3% of the 15–16 year-old population estimated as of 2022. THC has both acute and chronic effects through the dopaminergic and endocannabinoid systems. This study was conducted to better understand the metabolites and metabolic pathways in biological systems affected by cannabis, which may help find practical diagnostic and treatment approaches for people with cannabis dependence in the future. Metabolomic analysis of urine samples was performed using gas chromatography–mass spectrometry (GC–MS). MetaboAnalyst software was used to determine sample metabolite profiles, which were then subjected to multivariate statistical analysis. From data of over 200 metabolites in each sample of cannabis users, 92 metabolites with a p-value of less than 0.05 were selected for further analyses, of which 38 showed a decrease and 54 showed an increase compared to the nonuser group. Based on 43 metabolites (VIP > 1), subjected to MetaboAnalyst and CPDB, amino acid metabolism (especially arginine, methionine, and cysteine), vitamin metabolism (particularly biotin), and the urea cycle were the primarily affected metabolic pathways. The AUC values of the four metabolites (salsoline, 6-thiourate, procollagen 5-hydroxy-l-lysine, and biotin) with the highest VIP scores were between 0.93 and 0.98, with no significant difference. Metabolites with high VIP scores hold promise as biomarker candidates for identifying cannabis users, and the prominent pathways provide new insights into the understanding of the metabolic effects of cannabis.

Ethyl carbamate (EC), a carcinogen in alcoholic beverages, poses a potential hazard to human health. This study developed a gas chromatography-Fourier…

The research analyzed changes in lipids and volatile flavor substances in Tibetan dried pork slice (TDPS) under four baking methods: air fryer, oven b…

Mass spectrometry is a powerful technique for tear fluid proteomics, offering critical insights into its complex molecular composition. Traditional da…

Congenital adrenal hyperplasia (CAH) represents a group of inherited disorders affecting steroidogenesis. Early and accurate diagnosis is crucial for …

Cancer is the second leading cause of death in the United States, and with ongoing population growth and aging, its annual incidence continues to rise…

Nitroaromatic compounds, encompassing nitrophenols (NPs), nitrocatechols (NCs), and nitroguaiacols (NGs), have garnered significant attention due to t…

Emerging evidence highlights the distribution of D-amino acids (D-AAs) in the human body and their physiological significance in human health. However…

An innovative approach combining electrochemistry with online quadrupole time-of-flight-mass spectrometry (EC-MS/MS) was employed to study the oxidative products and metabolic pathways of three bioac...

The introduction of mass spectrometry in the analysis of pesticide residues has significantly enhanced analytical efficiency, reduced analysis times, and enabled ultra-trace detection. However, the m...

Validated assays are vital to support the accurate reporting of pharmacokinetics for parent drug/metabolite(s), regardless of the stage of drug development. One of the recent nuances in the field has...

Recent advancements in analytical techniques have significantly furthered our understanding of the chemical composition of petroleum and its derivatives. However, there remains a missing link connecting the molecular structure with how specific compounds are bound within the sample matrix. Traditional approaches to study energetic characteristics of petroleum often rely on bulk property measurements (e.g., thermogravimetric analysis), therefore lacking the resolution needed to capture compound-specific interactions and structural information. In this work, we aim to fill the gap between compositional and energetic analyses by employing thermal slicing ramped pyrolysis gas chromatography mass spectrometry (TSRP-GC-MS). TSRP-GC-MS enables the analysis of temperature-dependent release patterns of pyrolyzates, linking molecular structures to energetic characteristics via computing the energy distributions for specific pyrolyzates. Results of the photodegradation experiments demonstrate that as the photodegradation proceeds, the energy required to disrupt the matrix and to release n-alkanes increased from ca. 90 kJ/mol to over 100 kJ/mol, with significant increases for each individual n-alkane. The release energy of individual n-alkanes in crude oil and in tarball collected in the field were further compared in the second case study. The results not only showed an increase from 110 kJ/mol for n-alkanes in crude oil to 118 kJ/mol in tarball, but also revealed a homogenizing trend of the matrix strength of different n-alkanes in the latter. Overall, the proposed TSRP-GC–MS approach offers a powerful tool for advancing our understanding of petroleum and oil and has the potential to be expanded to the study of other complex natural mixtures.