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Bavarian Center for Biomolecular Mass Spectrometry @baybioms.bsky.social · Jul 31

We hope everyone enjoyed it as much as we did. Looking forward to seeing the next generation of #proteomics #scientists in 2026! 🚀

#TeamMassSpec #PhD #Postdoc #MassSpec #Research #Outreach

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Bavarian Center for Biomolecular Mass Spectrometry @baybioms.bsky.social · Jul 31

Proud, as always, to be part of the organizing team alongside fantastic #labs and colleagues. Special thanks to Tina (Dr. Christina Ludwig), our Proteomics Lead, for her dedication in making it all happen. 🙏👏

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Bavarian Center for Biomolecular Mass Spectrometry @baybioms.bsky.social · Jul 31

Beyond the lectures - from basics to the latest advances - there was plenty of time for social activities 🤝 and to enjoy Brixen’s stunning surroundings.🌲🏔️

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Bavarian Center for Biomolecular Mass Spectrometry @baybioms.bsky.social · Jul 31

🌄 The tradition continues! 🎉

This year’s #BrixenProteomics #SummerSchool once again exceeded expectations. We welcomed passionate young talents and leading experts from academia and industry for a week filled with science, inspiration, and collaboration. 🔬✨

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Bavarian Center for Biomolecular Mass Spectrometry @baybioms.bsky.social · Jul 22

🧵(6/6)

Last but not least, big thanks to our supporters: #ThermoFisherScientific and #SCIEX - sciex.bsky.social‬!#LifeSciencese#10YearsBayBioMSM#Academiai#Outreachc#PhDh#Postdoco#Researchch

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Bavarian Center for Biomolecular Mass Spectrometry @baybioms.bsky.social · Jul 22

🧵(5/6)

Whether you're a mass spectrometry enthusiast, #data #scientist, or #molecular #biologist, this symposium offers something for everyone passionate about proteomics and #biomolecular #research.

🔗 #Register now: lnkd.in/dV4Kf-Vh
📲 Or #scan the #QR code in the poster below.

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Bavarian Center for Biomolecular Mass Spectrometry @baybioms.bsky.social · Jul 22

🧵(4/6)

🌟 There will be additional talks by our #local "#heroes" in the #Bavarian #TeamMassSpec community!

A great mixture between all the pillars of our team, #Proteomics, #Metabolomics, #Bioinformatics, and applied #MassSpec techniques for a vast diversity of biological questions.

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Bavarian Center for Biomolecular Mass Spectrometry @baybioms.bsky.social · Jul 22

🧵(3/6)

🔬 We have put together a fascinating #Keynote #Speakers team:
• Prof. Albert Heck - hecklab.bsky.social‬
• Dr. Hamish Stewart - hamishs.bsky.social��
• Prof. Olga Vitek - olgavitek.bsky.social‬
• Prof. Ron M.A. Heeren

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Bavarian Center for Biomolecular Mass Spectrometry @baybioms.bsky.social · Jul 22

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🧪 #Join us for a full day of #science, #networking, and #inspiration on:
📅 22 #October 2025 | 🕣 8:30 – 18:30
📍 iGZW #Conference Room, TUM School of Life Sciences #Weihenstephan #Campus, Gregor-Mendel-Str. 4, 85354 Freising

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Bavarian Center for Biomolecular Mass Spectrometry @baybioms.bsky.social · Jul 22

🎉 BayBioMS turns 10! 🎉

2025 marks our 10th anniversary of Mapping Molecular Mountains.

Thus, we’re excited to invite you to the 10 Years of #BayBioMS #Symposium – a celebration of a decade of accelerating #MassSpectrometry research at the @tum.de!

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Bavarian Center for Biomolecular Mass Spectrometry @baybioms.bsky.social · Jul 1

This is a great opportunity to network with fellow researchers and learn about powerful applications of mass spectrometry in #proteomics, food chemistry, and neuroscience. See you there!

#MassSpectrometry #TeamMassSpec #Bioinformatics #Metabolomics

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Bavarian Center for Biomolecular Mass Spectrometry @baybioms.bsky.social · Jul 1

🔬 The next BayBioMS Advanced Mass Spectrometry Seminar is happening this Wednesday! 🧪

Doors open at 4:45 PM — free drinks and snacks will be served!

This time, we will highlight cutting-edge work in:

#Phosphoproteomics
(Targeted) #peptidomics
#PTM analysis (#citrullination)

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Reposted by Bavarian Center for Biomolecular Mass Spectrometry

Jakob Trendel @jtrend.bsky.social · May 23

Excited our paper is out in Cell @cp-cell.bsky.social!
🧬⚡ DNA photo-crosslinking proteomics in living cells
🎯 Pinpoints protein-DNA interactions to single amino acids
🌎 Globally quantifies DNA binding for >1800 proteins at a timescale of minutes
🔗 www.cell.com/cell/fulltex...
🧵

The human proteome with direct physical access to DNA

Zero-distance photo-crosslinking reveals direct protein-DNA interactions in living cells, enabling quantitative analysis of the DNA-interacting proteome on a timescale of minutes with single-amino-aci...

www.cell.com

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Reposted by Bavarian Center for Biomolecular Mass Spectrometry

Jakob Trendel @jtrend.bsky.social · May 23

UV crosslinking works great for protein-RNA complexes, but DNA is much less photo-reactive and photo-crosslinking not commonly used. Analogous, we find that the photo-activatable nucleotide 4ST (DNA) is about ten times less reactive than the commonly used 4SU (RNA). (1/n)

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Reposted by Bavarian Center for Biomolecular Mass Spectrometry

Jakob Trendel @jtrend.bsky.social · May 23

Unlike natural DNA, 4ST can be activated by wavelengths far outside the absorption range of most natural biomolecules. This avoids photodamage, allowing us to use high-power LEDs, with ~1000-fold higher intensity than conventional bulbs, for activating 4ST in living cells (www.uven.org). (2/n)

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Reposted by Bavarian Center for Biomolecular Mass Spectrometry

Jakob Trendel @jtrend.bsky.social · May 23

To analyze protein photo-crosslinked to DNA by proteomics, we develop XDNAX: protein-crosslinked DNA extraction. (3/n)

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Reposted by Bavarian Center for Biomolecular Mass Spectrometry

Jakob Trendel @jtrend.bsky.social · May 23

XDNAX extracts are very clean and we discover previously elusive peptide-DNA crosslinks within them. They pinpoint protein-DNA interaction sites to individual amino acids. (4/n)

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Reposted by Bavarian Center for Biomolecular Mass Spectrometry

Jakob Trendel @jtrend.bsky.social · May 23

In contrast to formaldehyde crosslinking, photo-crosslinking only captures direct interactions (‘zero-distance’). The proteins we find in close proximity to DNA show very high degrees of intrinsic disorder. In fact, intrinsic disorder rises the closer proteins localize to DNA. (5/n)

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Reposted by Bavarian Center for Biomolecular Mass Spectrometry

Jakob Trendel @jtrend.bsky.social · May 23

To identify transcription factors or chromatin modifiers responding to changes in a cell, DNA interactomes can be compared between conditions. Treating breast cancer cells with increasing estrogen doses, we find the estrogen receptor and other regulators recruited to DNA dose-dependently. (6/n)

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Reposted by Bavarian Center for Biomolecular Mass Spectrometry

Jakob Trendel @jtrend.bsky.social · May 23

This also works in primary cells, where we see recruitment of inflammation-associated transcription factors to DNA in macrophages challenged with LPS. (7/n)

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Reposted by Bavarian Center for Biomolecular Mass Spectrometry

Jakob Trendel @jtrend.bsky.social · May 23

We use these quantitative powers to profile three genotoxic drugs and find very distinct reactions in the DNA interactome of cancer cells. This reveals drug-specific vulnerabilities that can be exploited for combination treatments. (8/n)

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Reposted by Bavarian Center for Biomolecular Mass Spectrometry

Jakob Trendel @jtrend.bsky.social · May 23

DNA photo-crosslinking takes 1 minute on ice, while formaldehyde requires 10 minutes at room temperature. We record a time series of photo-crosslinked DNA interactomes during acute BAF inhibition and identify fast-acting restrictors of chromatin accessibility missed by conventional methods. (9/n)

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Reposted by Bavarian Center for Biomolecular Mass Spectrometry

Jakob Trendel @jtrend.bsky.social · May 23

Overall, we present a resource of nearly 50 photo-crosslinked, i.e. direct physical, DNA interactomes and identify 688 peptide-DNA crosslinks. These crosslinks mark known but also unknown protein-DNA interaction sites, some overlapping with variant sites linked to genetic or somatic disease. (10/n)

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Reposted by Bavarian Center for Biomolecular Mass Spectrometry

Jakob Trendel @jtrend.bsky.social · May 23

Very happy we pushed this across the finish line. Huge thanks to all the co-authors: Simon, Shuyao @shashuyao.bsky.social, the Greulich lab @franzigmuc.bsky.social, Susi, Karin @baybioms.bsky.social, Sandra, the Kubicek lab, the Uhlenhaut lab, and especially Bernhard @kusterlab.bsky.social. (11/11)

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Bavarian Center for Biomolecular Mass Spectrometry @baybioms.bsky.social · May 19

Many thanks to the organizers and the vibrant community for the inspiring discussions and connections.

#InPPO2025 #PlantProteomics #ECR #Proteomics #Bioinformatics #TeamMassSpec

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