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The bumbling biochemist (Bri Bibel)

@bumblingbiochemist.bsky.social

270 followers 420 following 420 posts

Assistant Prof of Biochemistry & Chemistry @ LMU | Metabolic enzymes of bioremediating bacteria. Making biochemistry fun & accessible #biochemsky #chemsky http://thebumblingbiochemist.com/ & https://www.youtube.com/@thebumblingbiochemist

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The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · Feb 12

Hi all. I'm the bumbling biochemist, on a mission to make biochemistry and chemistry fun and accessible for all. I have a blog & YouTube with a lot of content that can hopefully help people, especially biochemists-in-training. Hope it helps! thebumblingbiochemist.com

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biochemistry (hopefully) made accessible! You can also find lots of videos on my YouTube channel here - as in life - feel free to explore! My site is constantly a work in progress, so...

thebumblingbiochemist.com

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The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 11h

I love how my students, just like I did as a student, are falling in love with protein purification! And, they’re wanting to help others learn by starring in tutorial videos!

YouTube: youtu.be/ysuGCxME2lg

Today, I got to teach Korina about good chromatography column storage protocol.

Storing protein chromatography columns in 20% EtOH – key things to know

YouTube video by the bumbling biochemist

youtu.be

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 1d

Teaching undergraduates protein biochemistry is an immense privilege. Especially with this group of students!

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 2d

We keep our AKTA (FPLC machine for protein chromatography to purify proteins) in a deli fridge to protect the precious proteins we’re purifying! But it’s important to know that at temperatures that are low, you typically need to reduce your flow, so your runs will go slow! youtu.be/sTPyK0fF-Ys

Running FPLC (e.g. an AKTA) in the cold

YouTube video by the bumbling biochemist

youtu.be

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 3d

A “Quick Start Guide” for starting off in research with good habits

Some things I recommend you do/learn/etc. as early as possible in your training…

blog version: bit.ly/quickstartsc... ; downloadable: bit.ly/quickstartre...

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 3d

Someone had been asking about resources for starting researchers, so I thought I'd share this quick start guide to starting research with good habits. I updated it today to make sure it had the newest links and added a couple things. Hope it can help someone!

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The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 4d

Priming your pumps on an AKTA is important for removing air from them that can damage your system and mess up your chromatography run!
youtu.be/0br_kJTDFV4

Priming pumps on an AKTA

YouTube video by the bumbling biochemist

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The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 4d

Can't wait!

Ädam Ligeralde @aktaguy.bsky.social · 18d

Protein purification is evolving fast! Are your workflows keeping up? Join the first-ever Cytiva PRIME Summit in Boston to connect with peers, hear from experts & explore advances in purification, mRNA, automation & drug discovery.

Register for free 👉 buff.ly/Y2bySGe

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 5d

www.instagram.com/p/DPXyUaskWs...

Welcome back to Instagram. Sign in to check out what your friends, family & interests have been capturing & sharing around the world.

www.instagram.com

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 6d

Label your lids! One of the key lab tips I try to drill into my trainees: label the caps of tubes and bottles and all sides of boxes (and include date and initials) youtube.com/shorts/yjwkJ...

Label your lids!!!!

YouTube video by the bumbling biochemist

youtube.com

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 7d

Huge thanks to Devin Feil, Loyola Marymount University for the new headshots!

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 8d

I love getting to teach my students fun lab tricks. Today, 80% glycerol! The greatness of glycerol without the gunk! Try preparing an 80% glycerol solution. It’s way easier to work with! Here’s a practical look at how you can do it.
youtu.be/ATlsl42x_-A

Lab tip: 80% glycerol - you can pipet it easily and it's fun to make!

YouTube video by the bumbling biochemist

youtu.be

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The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 9d

Pretty protein purification peak :) youtube.com/shorts/0Yr0m...

Successful size exclusion chromatography run – nice symmetrical crisp peak :)

YouTube video by the bumbling biochemist

youtube.com

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 10d

ENZYME TIME!!!!!!!!!! Part 1: Enzyme Fundamentals youtu.be/5w8_zW396MA More on enzymes: bit.ly/enzymecataly...

Enzyme fundamentals (Enzymes Part 1)

YouTube video by the bumbling biochemist

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The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 11d

It's so fun teaching my lab members protein purification! Yesterday was injecting our protein sample into a sample loop, and starting SEC run on an AKTA. I'm working to build up a series of tutorial videos for my lab members, so thought I'd share here as well. youtu.be/r5AO5ojH5D8

Injecting sample into a sample loop and starting SEC run on an AKTA

YouTube video by the bumbling biochemist

youtu.be

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 12d

Today, the entire Bibel lab was finally here at the same time. And, my chemicals order arrived! It was a lot of fun! More about the Bibel lab and what we do at LMU: bibellab.lmu.build

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The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 13d

AKTA flow paths and injection valve modes – controlling the plumbing on an FPLC!

youtu.be/Wn0dRwwocOc

AKTA flow paths and injection valve modes – controlling the plumbing on an FPLC!

YouTube video by the bumbling biochemist

youtu.be

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 14d

Putting the chrom- in size exclusion chromatography (SEC) standards! Today we ran gel filtration standards on our size exclusion chromatography (SEC) column. And a couple of them (vitamin B12 and myoglobin) are colored! youtube.com/shorts/981nO...

Putting the chrom- in size exclusion chromatography (SEC) standards

YouTube video by the bumbling biochemist

youtube.com

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 15d

more on size exclusion chromatography: bit.ly/sizeexclusio...

Size exclusion chromatography (gel filtration)

No discussion of protein purifying would be complete if you excluded Size Exclusion Chromatography (SEC). Especially since, of all the types of protein chromatography you may be usin’, size exclusion ...

bit.ly

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 15d

Running size exclusion chromatography (practical look at running gel filtration on an AKTA PURE)
youtu.be/KJ4v1FwfyP4

Running size exclusion chromatography (practical look at running gel filtration on an AKTA PURE)

YouTube video by the bumbling biochemist

youtu.be

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 16d

The keys to connecting a SEC (Size Exclusion Chromatography)/Gel Filtration column to an FPLC: don't let in air; don't let the column get dry; don't let the pressure get too high. youtu.be/o0ya3EpM86o

Connecting a SEC (Size Exclusion Chromatography)/Gel Filtration column to an AKTA PURE

YouTube video by the bumbling biochemist

youtu.be

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 17d

Mind the gaps! Beware parts of the model may not be there! Scientists build structural models into experimental maps. Sometimes the experimental data is not strong enough in places, so the model has gaps! youtu.be/Vl_jcEx3jS4

Unmodeled regions of molecular structures (those grayed out regions and dashed lines in the models)

YouTube video by the bumbling biochemist

youtu.be

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 18d

Much more on recombinant protein expression and purification:
- Blog page of posts: bit.ly/proteinpurif...
- YouTube playlist: youtube.com/playlist?lis...

Protein expression and purification

Recombinant protein purification workflow Where’s the protein?! Troubleshooting and optimizing recombinant protein expression Protein chromatography Protein chromatography Protein pu...

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The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 18d

As I've begun building my lab and introducing students to protein purification, seeing their excitement, it's really hit home how incredible proteins are and how immense a privilege it is to get to explore them. I love feeling that I'm empowering my students with skills that can take them far.

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The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 19d

I have a feeling this desalting column is going to be one of the best uses of my start-up money! No more dialysis?! youtu.be/7Hn2_VtmFEo

Desalting columns for desalting and buffer exchange

YouTube video by the bumbling biochemist

youtu.be

The bumbling biochemist (Bri Bibel) @bumblingbiochemist.bsky.social · 20d

Approach minipreps in style, not dread :) bit.ly/minipreps

Alkaline lysis (mini preps)

Work in a lab & there are some “boring” experiments you have to do A LOT in order to do the “exciting” experiments that require more thought. An example many people might be overly-familiar with if th...

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