Also, I aliquot into 20-50ul and then freeze thaw. I haven't noticed issues with that (but also I don't freeze thaw more than a couple of times).

For yeast and bacteria I used it at 1:100, and that seemed to work well. I noticed that for mouse tissue it needs to be more concentrated.

It's supposed to be 200X, but for yeast and bacteria I treated it like 100X. When I use mouse tissue I use it a little more concentrated (some slight differences in concentration depending on which mouse tissue)

I've used this one since the yeast days. I freeze aliquots at -20 when I receive it. Ihttps://www.sigmaaldrich.com/US/en/product/sigma/p8340?srsltid=AfmBOoqGoHf_0TMrlS4hlQKjEOiQ9cbNeK261BYVt96d1ccKBKHkj1lA

What are you currently using?

thanks

It still bothers me *decades* later…. And that is why I stayed in science and didn’t go to law school… 😂

I took an lsat class decades ago and was shocked to learn that some can also mean all.

Good luck!🍀

I would love to be added if there is still space :)

Hi! I would love to join if there is still space :)