Dave Matus
@davematus.bsky.social
Researcher @ UC Berkeley Advanced BioImaging Center, MCB Department, Evo-devo-cell biologist, 🎷👨🍳🐕🐈⬛
You should! We’ve made a bunch of transgenics with it - it’s awesome! I can send you constructs if it’s helpful / just let me know!
December 6, 2025 at 5:34 PM
You should! We’ve made a bunch of transgenics with it - it’s awesome! I can send you constructs if it’s helpful / just let me know!
Reposted by Dave Matus
Any zebrafish people at ASCB interested in chatting about staygold or in vivo live imaging talk to Aline at her poster at P1238, and additional Swinburne lab posters as at P1238 (Maria) and P1229 on Tuesday
December 6, 2025 at 4:10 PM
Any zebrafish people at ASCB interested in chatting about staygold or in vivo live imaging talk to Aline at her poster at P1238, and additional Swinburne lab posters as at P1238 (Maria) and P1229 on Tuesday
Plasmids are enroute!
December 5, 2025 at 10:15 PM
Plasmids are enroute!
since you made it all the way to then end - we've already made transgenic lines with the mSG from Ando et al - it's amazing! here's a movie from our Cell Observatory project at the Advanced BioImaging Center 7/7
December 5, 2025 at 9:20 PM
since you made it all the way to then end - we've already made transgenic lines with the mSG from Ando et al - it's amazing! here's a movie from our Cell Observatory project at the Advanced BioImaging Center 7/7
We also looked at the new mGold2 variants - a bright new version of an mVenus derived FP - they are super bright (brighter than mNG when imaged with a 514nm laser), but show similar photostability to mNG. Plasmids should be available soon @addgene.bsky.social for all these FPs. 6/7
December 5, 2025 at 9:20 PM
We also looked at the new mGold2 variants - a bright new version of an mVenus derived FP - they are super bright (brighter than mNG when imaged with a 514nm laser), but show similar photostability to mNG. Plasmids should be available soon @addgene.bsky.social for all these FPs. 6/7
At medium and high laser powers the three mSG variants (mSG(E138D) -orange, mSG from Ando et al - red, mBaoJin - green) are all very photostable as expected compared to mNG (blue) 5/7
December 5, 2025 at 9:20 PM
At medium and high laser powers the three mSG variants (mSG(E138D) -orange, mSG from Ando et al - red, mBaoJin - green) are all very photostable as expected compared to mNG (blue) 5/7
but where these new FPs really shine is in photostability - we live imaged continuously for 800 frames in single FOVs 4/7
December 5, 2025 at 9:20 PM
but where these new FPs really shine is in photostability - we live imaged continuously for 800 frames in single FOVs 4/7
We found that the mStayGold variant published by Ando et al. was the brightest in our experiments (mSG(A) in the plot below) 3/7
December 5, 2025 at 9:20 PM
We found that the mStayGold variant published by Ando et al. was the brightest in our experiments (mSG(A) in the plot below) 3/7
We need to build lots of new transgenic lines that are both very bright and photostable, so I was excited to test membrane fusions of the three published mStayGold variants compared to mNeonGreen. We injected mRNA into zebrafish embryos and imaged at 24hpf. 2/7
December 5, 2025 at 9:20 PM
We need to build lots of new transgenic lines that are both very bright and photostable, so I was excited to test membrane fusions of the three published mStayGold variants compared to mNeonGreen. We injected mRNA into zebrafish embryos and imaged at 24hpf. 2/7
Strain boring : DQM, but I liked that Sherwood had picked his strain name (NK) after his kids, so I made my allele name (bmd) after my girls and wife (Bria, Maile, Deirdre)
June 3, 2025 at 2:46 AM
Strain boring : DQM, but I liked that Sherwood had picked his strain name (NK) after his kids, so I made my allele name (bmd) after my girls and wife (Bria, Maile, Deirdre)
We are looking for scientists with experience in light sheet microscopy, biological imaging, image processing and analysis to join a dedicated team of biologists, engineers and optical physicists that are pushing the boundaries of 4D in vivo imaging!
Come help us make movies like this!
Come help us make movies like this!
April 11, 2025 at 10:20 PM
We are looking for scientists with experience in light sheet microscopy, biological imaging, image processing and analysis to join a dedicated team of biologists, engineers and optical physicists that are pushing the boundaries of 4D in vivo imaging!
Come help us make movies like this!
Come help us make movies like this!
Jealous! Enjoy the gig!
April 11, 2025 at 3:09 PM
Jealous! Enjoy the gig!
Yes! Haven’t tested with lifeact yet but have been testing membrane and histone fusions of two new mScarlet3 derivatives - so far mScarlet3S2 is the winner / am sending some plasmids to @itsthewoolab.bsky.social happy to send!
March 18, 2025 at 1:34 AM
Yes! Haven’t tested with lifeact yet but have been testing membrane and histone fusions of two new mScarlet3 derivatives - so far mScarlet3S2 is the winner / am sending some plasmids to @itsthewoolab.bsky.social happy to send!
Can’t wait to come visit you in Vancouver!!!
March 5, 2025 at 1:22 AM
Can’t wait to come visit you in Vancouver!!!