Surprisingly, we detected active non-AUG translation from upstreamORF in viral transcripts. These translation events lead to the production of small peptides that induce a T cell response in infected individuals.
Our ribosome profiling results indicate translation of a fraction of the incoming viral mRNAs (as soon as 1h post-infection), confirming findings from a previous work (nature.com/articles/s41...).
Our results indicate a moderate effect of infection on overall mRNA translation rates but a strong effect on transcript and protein levels. Most effects are concordant but several functional classes of genes display discordant effects at the transcript and protein level.
