Many thanks to @sarah-guiziou.bsky.social and Cassandra Maranas for building out the amazing Integrase toolkit from which we sourced our parts, and to Dr. Jennifer Nemhauser for her unwavering support.

This tool builds on traditional T-DNA loss of functions, rescuing them with a switchable DNA target. This unlocks many essential genes for further study with just two additional T-DNAs. Inducible and cell type specific drivers allow testing in your tissues/times of choice.