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Mike Hough

@mikehough.bsky.social

310 followers 67 following 8 posts

Structural biologist and beamline scientist at Diamond light source, Hon Prof @ University of Essex. Interested in room temperature macromolecular crystallography, serial and time resolved work - and metalloproteins.

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Mike Hough @mikehough.bsky.social · Aug 22

Recent paper showing the power of combining advanced structural biology with QM/MM simulations to understand a heme enzyme. Work between Diamond, STFC, Bristol and Essex. We present evidence for the presence of an unusual protein to heme cross-link.
pubs.rsc.org/en/content/a...

pubs.rsc.org

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Mike Hough @mikehough.bsky.social · Aug 22

New paper describing use of photocaged nitric oxide for time resolved crystallography within fixed targets at XFEL and synchrotron - PhD work of Pete Smyth & Lewis Williams - great collaboration with @jarworrall.bsky.social, beamline I24 and SACLA. Funded by BBSRC
journals.iucr.org/m/issues/202...

Time-resolved serial synchrotron and serial femtosecond crystallography of heme proteins using photocaged nitric oxide

We demonstrate the application of a nitric oxide releasing photocage system for time-resolved serial crystallography studies of two heme containing proteins using a fixed target sample delivery system...

journals.iucr.org

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Mike Hough @mikehough.bsky.social · Jun 2

Paper describing clustering methods to separate subtle structural differences in populations of protein crystals - with application to polymorphs, ligand binding.. - led by Amy Thompson @amyjayyyne.bsky.social with VMXi,I24 and Data Analysis at @ddiamond.bsky.social journals.iucr.org/d/issues/202...

Enhanced intensity-based clustering of isomorphous multi-crystal data sets in the presence of subtle variations

Bovine, porcine and human insulin data sets are isomorphous in terms of unit cell and symmetry. Through enhancements to the multi-crystal clustering methods in DIALS, species-pure data sets are automa...

journals.iucr.org

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Mike Hough @mikehough.bsky.social · Apr 25

I'm awaiting the 24 hr rolling news coverage of the Conclave - where there is zero to actually report until smoke comes out of the chimney!

Mike Hough @mikehough.bsky.social · Apr 15

As part of this work we explicitly measured the XFEL temporal pulse profiles with a Wakefield device rather than inferring pulse length from electron bunch parameters

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Mike Hough @mikehough.bsky.social · Apr 15

Our new paper exploring the effect of XFEL pulse length and pulse intensity on electron density maps of two radiation sensitive proteins. A great collaboration between Diamond, SwissFEL and University of Essex's Worrall group.
journals.iucr.org/m/issues/202...

Damage before destruction? X-ray-induced changes in single-pulse serial femtosecond crystallography

Varied pulse-duration and pulse-intensity serial femtosecond crystallography data do not show significant signs of radiation damage under typical experimental conditions.

journals.iucr.org

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Mike Hough @mikehough.bsky.social · Mar 4

Nice to see our review on the fascinating cytochromes P460 and cytochromes c' - that use a similar and unusual structure to carry out very different functions out in JBIC. An idea for a mini review that expanded! link.springer.com/article/10.1...

Cytochromes P460 and c′-β: exploiting a novel fold for multiple functions - JBIC Journal of Biological Inorganic Chemistry

link.springer.com

Mike Hough @mikehough.bsky.social · Nov 26

Just getting started on blue sky - Using this mostly for work purposes, as a brief intro for people I don't already know - I'm a structural biologist at Diamond Light Source specialising in room temperature X-ray structures and serial crystallography at synchrotrons and XFELs