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shazebahmad.bsky.social
Shazeb Ahmad
@shazebahmad.bsky.social
34 followers 100 following 1 posts
Passionate about exploring RNA viral evolution and host-pathogen interactions. #RNAviruses #Phages
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shazebahmad.bsky.social
Shazeb Ahmad @shazebahmad.bsky.social · Jun 11
Excited to share my first-author publication from my #PhD work at @helmholtz-hiri.bsky.social 🎉

We established mudRapp-seq, a spatial transcriptomic technique that combines direct RNA padlock probing and in-situ sequencing.

academic.oup.com/nar/article/...
Visualizing the transcription and replication of influenza A viral RNAs in cells by multiple direct RNA padlock probing and in situ sequencing (mudRapp-seq)
Abstract. Influenza A viruses (IAVs) contain eight negative-sense single-stranded viral RNA (vRNA) molecules, which are transcribed into messenger RNA (mRN
academic.oup.com
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Reposted by Shazeb Ahmad
eddieholmes.bsky.social
Eddie Holmes @eddieholmes.bsky.social · Apr 8
Recombination between positive-sense and negative-sense RNA viruses: journals.plos.org/plospathogen...
A positive-sense single-stranded RNA virus acquired a negative-sense open reading frame through recombination
Author summary The discovery of brine shrimp virga-like virus 1 (BSVV1) challenges the conventional view that positive- and negative-sense RNA viruses are evolutionarily independent. Through genomic a...
journals.plos.org
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Reposted by Shazeb Ahmad
jorg-vogel-lab.bsky.social
Jörg Vogel @jorg-vogel-lab.bsky.social · Apr 9
If you want to do single-cell RNA-seq on your favourite bacterium, here‘s our detailed Nature Protocol for microbial MATQ-seq. It profiles up to 600 genes in a single bacterial cell!

www.nature.com/articles/s41...
Transcriptomic profiling of individual bacteria by MATQ-seq - Nature Protocols
Bacterial single-cell sequencing via MATQ-seq combines index sorting, random priming and rRNA depletion, and offers high cell retention and transcript capture rates, making it ideal for experiments wi...
www.nature.com
2 34 59
Reposted by Shazeb Ahmad
asherleeks.bsky.social
Asher Leeks @asherleeks.bsky.social · Dec 19
Phage coexistence, even when you least expect it.

Check out @norapyenson.bsky.social's study showing the repeated assembly of diverse phage communities: www.science.org/doi/10.1126/...

Also Nora's bsky thread here: bsky.app/profile/nora...

#SocialViruses #virosky #evosky 🧪🦠
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