Lattices is an optimal way of packing so perhaps it just packs this way to cram as many as possible on the membrane in a stable way....Anyways, always impressed with the Engel lab papers! In-situ structure work is the best kind of in-situ
September 24, 2025 at 6:22 PM
Lattices is an optimal way of packing so perhaps it just packs this way to cram as many as possible on the membrane in a stable way....Anyways, always impressed with the Engel lab papers! In-situ structure work is the best kind of in-situ
It has also worked for us for a small protein without using any scaffolding. Although we are careful with the model, we are quite impressed by the map quality achieved as we were able to dock it a refine it pretty well. With that said, we are cautious and eager to hear the whole story :)
September 21, 2025 at 10:15 PM
It has also worked for us for a small protein without using any scaffolding. Although we are careful with the model, we are quite impressed by the map quality achieved as we were able to dock it a refine it pretty well. With that said, we are cautious and eager to hear the whole story :)
I tried that but what worked the best was to germinate on agar germinating medium, then resuspend them gently and pipette them on grid. Not trivial but doable.
July 30, 2025 at 4:14 AM
I tried that but what worked the best was to germinate on agar germinating medium, then resuspend them gently and pipette them on grid. Not trivial but doable.
Yup. Have been saying this since Trump started overstepping. Where are all the wannabe heroes doing CQB training on weekends to prepare against the evil government? Prob no where because they are on the good side of the fence...for now...
July 13, 2025 at 6:21 AM
Yup. Have been saying this since Trump started overstepping. Where are all the wannabe heroes doing CQB training on weekends to prepare against the evil government? Prob no where because they are on the good side of the fence...for now...