William J Nicolas
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wjnicol.bsky.social
William J Nicolas
@wjnicol.bsky.social
Senior scientist @ Exelixis | Cryo-EM facility lead
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PhD @ CNRS | LBM-Bayer lab
Postdoc @ Jensen/Meyerowitz lab Caltech
Cryo-EM facility manager @ Gonen lab HHMI
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Website: www.wjnicolas.com
Twitter handle: @WiwiSFC
Makes sense! looks like limestone
November 10, 2025 at 6:24 AM
Where is that roof one with the city in the back drop? Cool contrast!
November 9, 2025 at 5:54 AM
Ah don't get me started!I miss climbing in the Sierras so much! Still waiting for my back to get better after my injury...
November 9, 2025 at 4:15 AM
Lattices is an optimal way of packing so perhaps it just packs this way to cram as many as possible on the membrane in a stable way....Anyways, always impressed with the Engel lab papers! In-situ structure work is the best kind of in-situ
September 24, 2025 at 6:22 PM
Very impressive tomograms. Those lattices on the thylakoid membranes...could almost do 2D xtallography...Beautiful work!
September 21, 2025 at 10:18 PM
It has also worked for us for a small protein without using any scaffolding. Although we are careful with the model, we are quite impressed by the map quality achieved as we were able to dock it a refine it pretty well. With that said, we are cautious and eager to hear the whole story :)
September 21, 2025 at 10:15 PM
Very interesting. Thanks
September 13, 2025 at 6:52 PM
Love the drawing!
August 24, 2025 at 1:40 AM
I tried that but what worked the best was to germinate on agar germinating medium, then resuspend them gently and pipette them on grid. Not trivial but doable.
July 30, 2025 at 4:14 AM
Congratulations!
July 29, 2025 at 8:05 PM
Great news ! Will give it a look. The tomograms look very nice!

I will release the data on the CZI public tomography portal
July 29, 2025 at 8:03 PM
Reminds me of the South Park episode where Randy tries to do the biggest poop in the world
July 16, 2025 at 11:06 PM
C'est a Arcachon ça ? Sur la promenade ? J'ai aussi cru a une vrai baleine. Mais vu les couleurs quand je me suis rapproché....
July 16, 2025 at 9:18 PM
Big incel power tripping vibs right here. Dangerous when those kinds of people hold that kind of power.
July 14, 2025 at 6:40 AM
Yup. Have been saying this since Trump started overstepping. Where are all the wannabe heroes doing CQB training on weekends to prepare against the evil government? Prob no where because they are on the good side of the fence...for now...
July 13, 2025 at 6:21 AM