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retof.bsky.social
Reto Fiolka
@retof.bsky.social
2.8K followers 1K following 640 posts
Optical imaging, interested in STEM topics. First generation graduate from ETH Zürich Associate Professor at UT Southwestern Medical Center
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 21h
Normal size and 4x EXM!
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 21h
Expansion microscopy is getting out of hand.

4X and 10X expanded wine barrels😳!

#SeeingIsBelieving
#EMBL
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 1d
Wow!
leonardo dicaprio is wearing a tuxedo and bow tie and smiling with the words yasssss written below him
ALT: leonardo dicaprio is wearing a tuxedo and bow tie and smiling with the words yasssss written below him
media.tenor.com
retof.bsky.social
Reto Fiolka @retof.bsky.social · 1d
The spectre of a post antibiotic world was always looming over us😞.
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 2d
Correct!
a woman in a car says " that 's on me , i set the bar too low "
ALT: a woman in a car says " that 's on me , i set the bar too low "
media.tenor.com
retof.bsky.social
Reto Fiolka @retof.bsky.social · 2d
DNA model with four base pairs, or ~2,6 kD.

#RookieNumbers
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 3d
Always keep PSA Flight 182 in mind😞:

en.m.wikipedia.org/wiki/Pacific...
Pacific Southwest Airlines Flight 182 - Wikipedia
en.m.wikipedia.org
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 3d
Retro Pacific Southwest Airlines livery on an AA Airbus 320.
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 4d
Yes, can be built upright.
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 4d
3D tracking of mitochondria DNA (green dots) in a HeLa cell.
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 4d
I grew up in Unterseen, not far away from Wengen. Went there for many hikes and skiing in winter. A truly magnificent place!

Thanks for sharing the pictures!
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 4d
Volumetric imaging of 384 wells in a contact free format using an air lens.

Importantly, the 3D imaging remains diffraction limited and distortion-free, despite the large refractive index mismatch from air to water.
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 4d
Segmented nuclei (gray) and nucleoli (color) in an expanded liver slice:
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 4d
Movies:

Microvili formation and dynamics in T-cells.
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 4d
Lastly, the zoom lens allows to adapt miOPM to samples with refractive indices (R.I.) different from water, such as cleared tissues.
Below is a comparison of doing CLARITY cleared imaging, with and without adapting the system to the different R.I.
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 4d
We can even use an air objective and still perform distortion free and diffraction limited imaging in watery samples.
This can be leveraged to perform contact-free multi-well imaging.
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 4d
The same system equipped with a water immersion objective can image larger samples, like an expanded liver slice, organoids or neurons on an EM grid.
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 4d
miOPM can use a high numerical oil immersion objective, and perform sensitive live-cell imaging, which we have leveraged to look at T-cell dynamics and track mitochondria DNA in 3D.
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 4d
@amsikking.bsky.social invented AIRR; it is an extension of remote focusing theory (Botcherby) in the presence of a refractive index mismatch. In practical terms, one can image distortion-free into a medium different from the immersion of the objective. This gives miOPM its objective flexibility.
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 4d
Unlike prior light-sheet systems, miOPM can use a variety of objectives, can be adapted to a wide range of refractive indices and is compatible with standard sample mounting. This comes from two innovations; the any immersion remote refocusing concept, and a zoom lens to adapt the system.
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 4d
We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues.
www.biorxiv.org/content/10.1...
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Reposted by Reto Fiolka
eurobioimaging.bsky.social
eurobioimaging.bsky.social @eurobioimaging.bsky.social · 10d
Meet Andres Bodén! In an interview with @virsicas.bsky.social, he explains how managing the Microscopy Innovation Centre at King's allows him to balance tech development with user projects — to shape the future of microscopy & broaden its impact. 🔬
More⤵️
www.eurobioimaging.eu/news/meet-an...
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 8d
Ahh, North Texas is just beautiful in Fall.

Soaking in the views from the Lewisville Landfill.
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 10d
Best of luck!
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retof.bsky.social
Reto Fiolka @retof.bsky.social · 10d
12 years at UT Southwestern Medical Center, and I still occasionally get lost on its campus.

This time I ended up at the unintentional Halloween portal.
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