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Reposted by Adam Hannan Parker

Gitta Coaker @gittacoaker.bsky.social · 19h

FRET sensor for SA developed. www.science.org/doi/10.1126/...

SALICYLIC ACID SENSOR1 reveals the propagation of an SA hormone surge during plant pathogen advance

Salicylic acid (SA) is a key phytohormone that orchestrates immune responses against pathogens, including Pseudomonas syringae bacteria. The timing and extent of SA accumulation are tightly controlled...

www.science.org

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Reposted by Adam Hannan Parker

Pierre Baduel @pierrebaduel.bsky.social · 21d

Happy to share the results of a long-haul post-doc project, now online @science.org, aiming at understanding the rules of transgeneration epigenetic inheritance over TEs in plants and its extent and impact in nature. More below!
doi.org/10.1126/scie...

Transposable elements are vectors of recurrent transgenerational epigenetic inheritance

DNA methylation loss at transposable elements (TEs) can affect neighboring genes and be epigenetically inherited in plants, yet the determinants and significance of this additional system of inheritan...

doi.org

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Adam Hannan Parker @adamahp.bsky.social · 23d

Interesting!

www.nature.com/articles/s41...

#plantscience #epigenetics

Gene body methylation regulates gene expression and mediates phenotypic diversity in natural Arabidopsis populations - Nature Plants

This study shows that epigenetic gene body DNA methylation regulates transcription and accounts for much of its variance in the Arabidopsis population, efficiently identifies genes regulating diverse ...

www.nature.com

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Adam Hannan Parker @adamahp.bsky.social · Aug 21

forgot to include #plantscience #plantimmunity #epigenetics !

Adam Hannan Parker @adamahp.bsky.social · Aug 19

Very excited to finally share the main findings from my PhD, now out as a preprint!

We developed an epigenetic system to understand how plants remember and forget past infections - a phenomenon known as immune memory 🌱🧬🧠

Highlights below! 1/9

www.biorxiv.org/content/10.1...

From recall to reset: the role of DNA (de)methylation in modulating plant immune memory

Epigenetic reprogramming is increasingly recognised as a driver of plant immune memory, yet its molecular basis remains poorly understood. Using a chemically inducible transgene in Arabidopsis, we tra...

www.biorxiv.org

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Adam Hannan Parker @adamahp.bsky.social · Aug 19

It's been a massive effort with help from so many people. Thank you @jurriaanton.bsky.social, @plantylisasmith.bsky.social, and all other co-authors not on Bluesky. Thank you @psaima.bsky.social and @dcb40.bsky.social for some very useful conversations during my viva! 9/9

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Adam Hannan Parker @adamahp.bsky.social · Aug 19

This work sheds new light on ROS1 (the best plant protein?), uncovering new and exciting genomic targets. Beyond Arabidopsis, we’re also excited by the translational potential of this system, providing a new way to introduce epigenetic variation into other plant species 🌱✨
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Adam Hannan Parker @adamahp.bsky.social · Aug 19

We needed to block the hypermethylation building up in peri/centromeres. Our genetic attempts hit roadblocks, so we switched to a pharmacological approach: 5-Aza, a DNA methylase inhibitor.
Remarkably, 5-Aza not only boosted ROS1-induced resistance to Pst-Lux, it prolonged the immune memory!
7/9

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Adam Hannan Parker @adamahp.bsky.social · Aug 19

This got us thinking….

Hypomethylation in (peri)centromeres is associated with resistance to the same pathogens that ROS1 immune memory resists (Furci et al 2019; Cambiagno et al 2018). So, could this re-methylation response in the (peri)centromeres be negatively regulating immune memory?
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Adam Hannan Parker @adamahp.bsky.social · Aug 19

ROS1 caused widespread demethylation and small RNA loss in chromosome arms, *but* in the highly repetitive pericentromeric/centromeric regions we saw the opposite — increases in DNA methylation and small RNAs.
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Adam Hannan Parker @adamahp.bsky.social · Aug 19

The obvious question: how? To tackle this, we did small RNA sequencing, DNA methylation sequencing (short-read and long-read), and RNA sequencing. What we saw was surprising….
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Adam Hannan Parker @adamahp.bsky.social · Aug 19

Bursts of ROS1 activity robustly induced resistance against the pathogenic bacteria Pst-Lux and the oomycete pathogen Hpa.

This immune memory, generated by ROS1, lasted 1-2 weeks, providing an ideal system to study both the establishment and erasure of immune memory.

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Adam Hannan Parker @adamahp.bsky.social · Aug 19

In Arabidopsis, the removal of an epigenetic mark, DNA methylation, often occurs in response to pathogen infection or abiotic stress.
We used an estradiol-inducible gene construct for the DNA demethylase ROS1 to transiently increase demethylation activity in plants.
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Adam Hannan Parker @adamahp.bsky.social · Aug 19

Very excited to finally share the main findings from my PhD, now out as a preprint!

We developed an epigenetic system to understand how plants remember and forget past infections - a phenomenon known as immune memory 🌱🧬🧠

Highlights below! 1/9

www.biorxiv.org/content/10.1...

From recall to reset: the role of DNA (de)methylation in modulating plant immune memory

Epigenetic reprogramming is increasingly recognised as a driver of plant immune memory, yet its molecular basis remains poorly understood. Using a chemically inducible transgene in Arabidopsis, we tra...

www.biorxiv.org

2 5 16

Adam Hannan Parker @adamahp.bsky.social · Jul 28

www.biorxiv.org/content/10.1...

very cool work!

Somatic mobility of transposons is explosive and shaped by distinct integration biases in Arabidopsis thaliana

Background The movement of transposable elements (TEs) in somatic cells generates genetic and phenotypic diversity across the soma of individual organisms. This process is especially important in plan...

www.biorxiv.org

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Reposted by Adam Hannan Parker

Plants, Photosynthesis and Soil @sheffieldpps.bsky.social · Jun 18

Plants remember stress! The PlantMemo project, led by ‪@jurriaanton.bsky.social‬, has secured an ERC Advanced Grant for an exciting project decoding how stress “memories” boost plant resilience—paving the way for hardier crops.
🔗 www.sheffield.ac.uk/biosciences/...

#PlantScience #PlantImmunity #ERC

New research aims to unlock stressful plant memories

New research led by Prof Jurriaan Ton aims to uncover how plants adapt and survive in challenging environments with the ultimate goal to create more resilient crops.

www.sheffield.ac.uk

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Reposted by Adam Hannan Parker

Marc Somssich @somssich.bsky.social · May 6

📜 Herbivory-induced green leaf volatiles increase plant performance through jasmonate-dependent plant–soil feedbacks

🧑‍🔬 Lingfei Hu, Meng Ye, @matthiaserb.bsky.social, Jianming Xu, et al.

📔 @natplants.nature.com

🔗 www.nature.com/articles/s41...

#️⃣ #PlantScience #PlantImmunity #Herbivory

Herbivory-induced green leaf volatiles increase plant performance through jasmonate-dependent plant–soil feedbacks - Nature Plants

This study reports that herbivory-induced volatiles can trigger plant–soil feedbacks that boost performance and yields of cereals in the field.

www.nature.com

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Reposted by Adam Hannan Parker

jameswalker303.bsky.social @jameswalker303.bsky.social · Apr 9

I'm thrilled to share that our study is now published in Cell:
Extensive N4 cytosine methylation is essential for Marchantia sperm function.
www.cell.com/cell/fulltex...
This paper confirms our 4mC discovery in Marchantia sperm and takes it much further.
A thread: 0/13

Extensive N4 cytosine methylation is essential for Marchantia sperm function

Global N4 cytosine methylation in Marchantia polymorpha sperm regulates gene expression and promotes sperm fertility.

www.cell.com

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Reposted by Adam Hannan Parker

Ka Wai Ma @kawaima.bsky.social · Apr 1

‼️we are pleased to share the first work coming from my lab on biorxiv.

Misregulation of the jasmonate signaling pathway leads to altered plant microbiota interaction and plant stress responses biorxiv.org/content/10.1...

1/n

Misregulation of the jasmonate signaling pathway leads to altered plant microbiota interaction and plant stress responses

The model plant Arabidopsis thaliana hosts diverse microbial communities collectively known as the microbiota. The plant microbiota is generally taxonomically structured. Some of the members can promo...

biorxiv.org

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Adam Hannan Parker @adamahp.bsky.social · Mar 25

thanks! will have a look

Adam Hannan Parker @adamahp.bsky.social · Mar 25

get the impression continuous light is a bit risky from this. thanks!

Adam Hannan Parker @adamahp.bsky.social · Mar 25

does Arabidopsis thaliana cycle through generations faster in 24h light or is 16h optimal? struggling to find any papers on this .. #plantsci

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Adam Hannan Parker @adamahp.bsky.social · Mar 13

www.nature.com/articles/s41...

#plantscience

Salt stress activates the CDK8-AHL10-SUVH2/9 module to dynamically regulate salt tolerance in Arabidopsis - Nature Communications

The authors demonstrate that salt stress activates CDK8, which phosphorylates AHL10 and promotes its degradation. AHL10 is involved in recruiting SUVH2/9 to repress salt-responsive genes. The CDK8-AHL...

www.nature.com

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Reposted by Adam Hannan Parker

Jake Harris @c-jake-harris.bsky.social · Feb 12

Very excited to share this new preprint from the lab (shorturl.at/l7lNq)! @hendersi.bsky.social si.bsky.social @camplantsci.bsky.social

CRISPR targeting of H3K4me3 activates gene expression and unlocks centromeric crossover recombination in Arabidopsis

H3K4me3 is a fundamental and highly conserved chromatin mark across eukaryotes, playing a central role in many genome-related processes, including transcription, maintenance of cell identity, DNA dama...

shorturl.at

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Adam Hannan Parker @adamahp.bsky.social · Feb 15

Cool work from Jacobsen and colleagues.
Great to finally have published and public ROS1 Chip-Seq data !

#PlantScience

www.nature.com/articles/s41...

Histone H3 lysine 4 methylation recruits DNA demethylases to enforce gene expression in Arabidopsis - Nature Plants

This study revealed that targeting H3K4me3 via the H3K4 methyltransferase SDG2 activates gene expression and removes DNA methylation by recruiting DNA demethylases. Conversely, the removal of H3K4me3 ...

www.nature.com

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Reposted by Adam Hannan Parker

Ian Henderson @hendersi.bsky.social · Jan 2

Centrophilic retrotransposon integration via CENH3 chromatin in Arabidopsis from Tetsuji Kakutani & colleagues - beautiful insights into control of transposon insertion into the centromeres
www.nature.com/articles/s41...

Centrophilic retrotransposon integration via CENH3 chromatin in Arabidopsis - Nature

An Arabidopsis long terminal repeat retrotransposon integrates de novo into regions occupied by centromere-specific histone variant, showing the impact of centromeric chromatin on transposable element...

www.nature.com

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