The rise of biosimilars demands precise 𝐠𝐥𝐲𝐜𝐨𝐬𝐲𝐥𝐚𝐭𝐢𝐨𝐧 analysis, but traditional methods are slow & error-prone.
🔬 This study introduces a Python-based tool 🖥️ for fast GI estimation.
✅ High accuracy via XGBoost ML
✅ Strong GI for trastuzumab (>87%) & bevacizumab (>92%)
🔗 https://buff.ly/3Eu1kIu

🔬 NKCE are a next-gen cancer immunotherapy, enhancing NK cell activation via multi-receptor engagement. They improve tumor killing over traditional Abs. Clinical trials show promise, with ongoing innovations optimizing efficacy, persistence & combo potential. #Immunotherapy
🔗 https://buff.ly/3Erq4B3

🧬 sgRNAs are key for CRISPR but hard to analyze. This study presents an ion-pairing RP chromatography method with single-nucleotide resolution. Octylamine/HFIP improves impurity detection, enhances MS compatibility & enables QC in sgRNA manufacturing.
🔗 https://buff.ly/40XU803

Nice to see SFC applied to biomolecules! This study shows SFC effectively separates deaminated ON impurities. With growing ON therapeutics, precise impurity analysis is key. SFC offers sharp resolution, unique selectivity & optimization potential! 🚀🔬
🔗 https://buff.ly/4aKrhzG

Phosphorothioate mods enhance CRISPR sgRNA but create isomers that complicate analysis. Regeneron’s new method uses endonuclease digestion, RP-LC & cyclic IMS to resolve PS sites. It enables isomer separation, precise quantification & stability insights for oligo QC!
🔗 https://buff.ly/4gr6Etp

🚀 Slalom Chromatography (SC) 🏁 is making a comeback! Unlike SEC, SC enables superior size-based separation of large DNA/RNA, resolving dsRNA impurities & plasmid DNA integrity for gene therapies. Optimized with UHPLC, it outperforms traditional methods.
🔗https://buff.ly/40SI8Nb

Ensuring high-quality in vitro-transcribed mRNA is crucial for stability & translation in therapeutics. This study presents a DNAzyme-based one-step method for simultaneous analysis of 5’-capping & 3’-poly(A) tail length—eliminating complex workflows & improving QC.
🔗 https://buff.ly/4hjL5fp

The rise of mRNA therapeutics demands robust QC. LC techniques like IP-RP, AEX & SEC enable precise analysis of mRNA integrity, capping efficiency & poly(A) tail characteristics. As mRNA expands into cancer & autoimmune therapies, strong analytical workflows are key. https://buff.ly/4gpGIOK

🚀 Scienta Lab & OSE Immunotherapeutics team up to harness AI-driven immunology! 🔬🤖 Their model, EVA, will:
✅ Stratify immune diseases
✅ Identify predictive biomarkers
✅ Enable targeted treatments
AI is accelerating innovation in drug development!
🔗 Read more:

HILIC 🧬 is a strong alternative to IP-RP-LC for ON analysis, requiring careful method development. This guide covers key aspects like stationary phase selection, mobile phase optimization, and MS considerations.
🔗 Read more: https://buff.ly/40WZKXh 🚀

🧬 Therapeutic oligonucleotides need precise P-chirality control for 🔬 stability & ⚡ efficacy. Phosphorothioate (PS) bonds create diastereomers, affecting drug performance. 🛠️ Advanced analytics like ion mobility spectrometry & chromatography ensure consistency. 📏
📖👉 https://buff.ly/3CwL60z

🚨 Call for contributions!
📚 Editing a book on analytical characterization of AAVs (Methods in Molecular Biology – Springer). Still open for protocol chapters! Submision of manuscripts by end of April. Contact me if interested.
🌟 #AAV #GeneTherapy #Science

🔬 New workflow for AAV capsid protein analysis! MauriceFlex icIEF fractionation + LC-MS enables precise separation & characterization of charge variants, advancing PTM monitoring & gene therapy development. Optimized for stability, recovery & quality. #AAV #GeneTherapy
🔗 doi.org/10.1002/elps...

🔬 Extensive Study on IgG1 Glycosylation 🧬
Multivariate analysis reveals how glycan patterns shape mAb functions: 1️⃣ Afucosylation boosts ADCC 🚀 2️⃣ Galactosylation enhances complement activation 🛡️ 3️⃣ Statistical models enable precise control under QbD. 🎯
#Biopharma #Glycosylation
doi.org/10.1080/1942...

🧪 New tools are uncovering "zero-dalton" PTMs like D-amino acids (DAACPs) & isoaspartate (IACPs), crucial for peptide stability, aging, & disease. Advances in MS, IMS & bioinformatics boost detection despite no mass shift. A leap for proteomics! 🌟 #MassSpec #Proteomics #PTMs
doi.org/10.1002/mas....

🎉 @fda.gov approves Jazz Pharmaceuticals' Ziihera® (zanidatamab), the 1st biparatopic antibody for HER2+ cancers! A breakthrough in oncology, combining CDC, ADCC, & phagocytosis for superior efficacy. New hope for resistant cancers! Learn more: investor.jazzpharma.com/news-release...

🔬 Advancing mAb charge profiling: Non-linear calibration in icIEF minimizes biases, ensuring precise pI measurements. UED optimizes resolution, enabling reproducible, tailored methods. A step closer to robust, device-agnostic QC for mAbs! 💡 #Biopharma #icIEF #mAbs #QC
doi.org/10.1038/s415...

🚨 We’re hiring! R&D Scientist – Molecular & Cellular Biology 🧬
Join us to develop cutting-edge methods for innovative medicines. PhD/Master’s in Bio required + skills in Flow Cytometry, qPCR/ddPCR & cell culture. Apply now! 🌟
Details: www.quality-assistance.com/jobs/rd-scie...
#JobAlert #Innovation

Honored to join the Peer Exchange panel on "𝑆𝑡𝑎𝑡𝑒 𝑜𝑓 𝑡ℎ𝑒 𝐴𝑟𝑡 𝑖𝑛 𝑂𝑙𝑖𝑔𝑜𝑛𝑢𝑐𝑙𝑒𝑜𝑡𝑖𝑑𝑒 𝐴𝑛𝑎𝑙𝑦𝑠𝑖𝑠" with Claus Rentel & Mark Dickman, moderated by Daniel Meston. 🧬 Insights now live! Watch here: www.chromatographyonline.com/peer-exchang... 🔍 Let’s discuss the future of ON analysis! 🚀

Excited to join the 𝐖𝐚𝐭𝐞𝐫𝐬 𝐀𝐐𝐛𝐃 𝐒𝐲𝐦𝐩𝐨𝐬𝐢𝐮𝐦 on Dec 3-4! I'll discuss 𝐋𝐂 𝐦𝐞𝐭𝐡𝐨𝐝 𝐬𝐭𝐫𝐚𝐭𝐞𝐠𝐢𝐞𝐬 in the 𝐐𝟏𝟒 era with James Sweatman, Jerome Pretre & Ilse Hoes, PhD. Don’t miss it—register now! 💻 Free & virtual: lnkd.in/eABzDcsh 🚀 #AQbD

Therapeutic oligonucleotides are revolutionizing medicine! 💊 Precise analysis is key to ensuring quality. This study highlights how 𝐈𝐏-𝐑𝐏𝐋𝐂 enables ultra-fast ON separation using optimized conditions (short columns, tailored IP reagents). Fast QC for scalable production. 🚀

doi.org/10.1016/j.ch...