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BaglaenkoLab

@baglaenkolab.bsky.social

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The Baglaenko Lab of Immunogenetics in the Division of Human Genetics at Cincinnati Children's Hospital is focused on developing new experimental and computational approaches to answer the most difficult questions in inflammatory and infectious diseases.

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Nature @nature.com · Jul 29

Nature research paper: Precisely defining disease variant effects in CRISPR-edited single cells

go.nature.com/44M3ygX

Precisely defining disease variant effects in CRISPR-edited single cells - Nature

A plate-based assay called CRAFTseq has been developed that uses ‘multi-omic’ single-cell RNA sequencing and direct genotyping of CRISPR edits to test the functional effects of genetic variants on cell state and function.

go.nature.com

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BaglaenkoLab @baglaenkolab.bsky.social · Jul 29

Author here to answer any questions. The full protocol is online at protocols.io and all codebooks on github

Bring structure to your research - protocols.io

A secure platform for developing and sharing reproducible methods.

protocols.io

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BaglaenkoLab @baglaenkolab.bsky.social · Jul 23

Many many thanks to Soumya Raychaudhuri, Phoenix Mu, and every other co-author on this project. We are continuing to scale and implement these strategies in my lab, so reach out if that interests you.

BaglaenkoLab @baglaenkolab.bsky.social · Jul 23

Happy to answer any and all questions about this work. We'd love to see more people try it.
Full protocol: www.protocols.io/view/craftse...
Full analysis: github.com/immunogenomi...

CRAFTseq

Genetic studies have identified thousands of individual disease-associated non-coding alleles, but identification of the causal alleles and their functions remain critical bottl...

www.protocols.io

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BaglaenkoLab @baglaenkolab.bsky.social · Jul 23

This plate based approach is highly adaptable and easy (enough) to implement without specialized equipment. We show that this is a robust and reliable way to functionally validate causal genetic variants directly in CRISPR editing cells.

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BaglaenkoLab @baglaenkolab.bsky.social · Jul 23

I am very excited to share our publication, out now in Nature, detailing our developed CRAFTseq methodology for capturing and analysing single cell genomic DNA amplicons alongside the transcriptome and cell surface protein expression.

www.nature.com/articles/s41...

Precisely defining disease variant effects in CRISPR-edited single cells - Nature

A plate-based assay called CRAFTseq has been developed that uses ‘multi-omic’ single-cell RNA sequencing and direct genotyping of CRISPR edits to test the functional effects of genetic variants o...

www.nature.com

1 1 4

BaglaenkoLab @baglaenkolab.bsky.social · Jun 2

My lab thinks a lot about CRISPR editing variants of interest in primary human cells. We made a little tool to help. Hopefully, someone else can get some use out of it. baglaenkolab.shinyapps.io/snpGuide/
github.com/ybaglaenko/s...

shiny.io