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Filiz Kuybu @filizkuybu.bsky.social · 17d

Thanks Ria 😊

Filiz Kuybu @filizkuybu.bsky.social · 17d

Thank you, Seychelle! 😊

Filiz Kuybu @filizkuybu.bsky.social · 19d

It’s been a long time coming! Grateful to all co-authors and colleagues who supported this study, especially @yilanfan.bsky.social 🙌 We have been working on this complex for nearly 3 years together and finally managed to tame it. MRN gave us Many Restless Nights, but we’re More Relaxed Now 😎

Hopfner Lab @hopfnerlab.bsky.social · 21d

Thrilled that our work is now finally out in Nature Comms!
rdcu.be/eG3vP

We reveal cryo-EM structures of the MRN complex bound to DNA & TRF2 - showing how DNA breaks are sensed and regulated at telomeres.
Fantastic work by first authors @yilanfan.bsky.social @filizkuybu.bsky.social & Hengjun!

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Reposted by Filiz Kuybu

Hopfner Lab @hopfnerlab.bsky.social · Aug 27

Check out our pre-print on how the human INO80 complex acts on nucleosomes and hexasomes: PostDocs Priyanka & Manmohan et al. solved structures of INO80 bound to several substrates and completed missing questions about similarities of modes of actions across species🧬 www.biorxiv.org/content/10.1...

Recognition and remodelling of nucleosomes and hexasomes by the human INO80 complex

The ATP-dependent INO80 chromatin remodeller slides and repositions nucleosomes to shape and maintain chromatin around gene regulatory elements and replication origins. Recent work uncovered capabilit...

www.biorxiv.org

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Reposted by Filiz Kuybu

Kelly Nguyen @kellythd-nguyen.bsky.social · Jul 10

Proud to share our work where we resolved a longstanding question regarding the existence of a human telomerase dimer and provided insights into its function. Led by 3 amazing lab members in collaboration with @yiliangding.bsky.social and @rdaslab.bsky.social.
www.science.org/doi/10.1126/...

Cryo-EM structure of human telomerase dimer reveals H/ACA RNP-mediated dimerization

Telomerase ribonucleoprotein (RNP) synthesizes telomeric repeats at chromosome ends using a telomerase reverse transcriptase (TERT) and a telomerase RNA (hTR in humans). Previous structural work showe...

www.science.org

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Reposted by Filiz Kuybu

Gene Center Munich @LMU Munich @genecenter-lmu.bsky.social · May 6

📢Stigler lab in collaboration with the Holt lab decipher that LINE-1 ORF1p forms super-saturated co-condensates with RNA that enable DNA binding and nuclear entry during mitosis.
Congratulations!👏👏👏
Details: www.science.org/doi/10.1126/...

LINE-1 ribonucleoprotein condensates bind DNA to enable nuclear entry during mitosis

ORF1p forms condensates that bind to chromosomes during cell division, allowing L1 elements to insert themselves into the genome.

www.science.org

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Reposted by Filiz Kuybu

duderstadtlab.bsky.social @duderstadtlab.bsky.social · Apr 9

🚨New preprint🚨We developed a transverse flow imaging approach to directly visualize bacterial replisomes challenged by topological barriers and discovered a fast rotational mode of replisome operation. Fantastic work by thomasmretzer.bsky.social and Hamdan Lab! tinyurl.com/replisomerot...

Real-time imaging of rotation during synthesis by the replisome

During chromosome replication, unwinding by the helicase and synthesis by the polymerases can lead to overwinding and supercoiling of DNA. The mechanical consequences of these events and resulting loc...

tinyurl.com

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Reposted by Filiz Kuybu

Hopfner Lab @hopfnerlab.bsky.social · Mar 17

Happy to share our latest pre-print!
We solved the cryoEM structures of human DNA repair factor MRN bound to DNA and to TRF2. Main work of Yilan, Filiz @filizkuybu.bsky.social and Hengjun. Great collab within the @sfb1361.bsky.social and @genecenter-lmu.bsky.social 🧬🔨
www.biorxiv.org/content/10.1...

Structural basis for DNA double-strand break sensing by human MRE11-RAD50-NBS1 and its TRF2 complex

The MRE11-RAD50-NBS1 (MRN) complex is a central, multifunctional factor in the detection, signaling and nucleolytic processing of DNA double-strand breaks (DSBs). To clarify how human MRN binds generi...

www.biorxiv.org

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Reposted by Filiz Kuybu

Julian Stingele @jstingele.bsky.social · Feb 20

Happy to share our review on the cellular responses to RNA damage 🥂

Congrats to joined first authors Jacqueline Cordes & @shubozhao.bsky.social

www.cell.com/cell/fulltex...

Cellular responses to RNA damage

RNA plays a central role in protein biosynthesis and performs diverse regulatory and catalytic functions, making it essential for all processes of life. Like DNA, RNA is constantly subjected to damage...

www.cell.com

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Reposted by Filiz Kuybu

Hopfner Lab @hopfnerlab.bsky.social · Dec 3

Hello, Bluesky!
To kick things off here, we’re excited to share: The SLFN11 story continues! We combined cryo-EM and biochemical assays to explain how phosphorylation regulates SLFN11´s tRNA cleavage and ssDNA binding activities.

Check out our new paper here www.nature.com/articles/s41... + movie

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