Flavio Zolessi
@fzolessi.bsky.social
480 followers 540 following 39 posts
Associate Professor of Cell Biology, Facultad de Ciencias, Udelar, Uruguay. PI, Cell Biology of Neural Development group. #development #retina #neurulation #zebrafish Member of IBRO-LARC @ibroorg.bsky.social
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fzolessi.bsky.social
Being new to this network, a presentation is in order. This will eventually become a thread. I first want to acknowledge these guys, the members of the Cell Biology of Neural Development group, of which I am privileged to be the current PI. #neurodevelopment #retina #neurulation #zebrafish #chick
The current members of the Cell Biology of Neural Development (CBDN) group, at Facultad de Ciencias, UdelaR, Uruguay. From left to right, top-down: Lucía Veloz (PhD student), Camila Davison (Associate Researcher), Gonzalo Aparicio (@gaparicio.bsky.social, PhD student), Magela Rodao (PhD student), Lucía Mustto (Master student), Santiago Bosch (Master student, recently finished), me, Agustín Siciliano (undergraduate student). Image taken a couple of weeks ago, at the XX Meeting of the Uruguayan Society for Neuroscience.
fzolessi.bsky.social
Recent publication from a great collaboration with my friends in Cuernavaca, México: The Smarce1 subunit of the BAF complex performs distinct, stage-specific functions during zebrafish retinal development doi.org/10.1016/j.ne.... Support @ibroorg.bsky.social, publish in @ibrojournals.bsky.social
Severe microphthalmia and retinal layering defects in the smarce1-/- zebrafish mutant.
Reposted by Flavio Zolessi
biorxiv-devbio.bsky.social
Mechanical conditions preventing live cell extrusion during primary neurulation in amniotes https://www.biorxiv.org/content/10.1101/2025.08.08.668972v1
Reposted by Flavio Zolessi
ibrojournals.bsky.social
📣 Call for #PAPERS!
Share your work in #IBRO #Neuroscience Reports’ special issues on early career #research and on perspectives from #AfrAbia! 🧠🌍

Learn more: www.ibroneuroreports.org/call-for-pap...

#IBROjournals #IBROauthors @ibroorg.bsky.social
Reposted by Flavio Zolessi
ibroorg.bsky.social
Are you a woman #neuroscientist eager to share your research and inspire others on stage? 🎤
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fzolessi.bsky.social
I just realized Santiago is in Bluesky! You can contact him here: @sabr-bio.bsky.social
fzolessi.bsky.social
I personally thank Santi's thesis co-supervisor, Julio A. Hernández. This work was possible thanks to our great graduate program PEDECIBA; to Universidad de la República, which funded research through CSIC and SB fellowship through CAP; to the Advanced Bioimaging Unit at Institut Pasteur Montevideo.
fzolessi.bsky.social
A reduction in apical/basal tension, on the other hand, would produce extensive cell extrusion after a period of relative stability. When extrapolated to a living tissue, these observations could be explained by cell-cell and cell-matrix adhesion, relying on F-actin-generated tension across cells.
fzolessi.bsky.social
Making a long story short, and skipping the quantitative part (if interested, ask Santi), what we found is that the relatively crowded simulated pseudostratified epithelium was stable as long as apical/basal tension was kept high relative to lateral tension. An example is reproduced in the video.
fzolessi.bsky.social
Here is where theoretical modelling enters the story. The goal was to simulate a pseudostratified epithelium using an energy-based 2D vertex model to get cells with flexible borders, able to respond to the changing positions of non-deformable nuclei. The "fake neuroepithelium" is seen from a side.
Diagrams illustrating the different simulations considered in the analysis of the energy model. A. State of a typical system at time t as obtained by dynamical simulation. Vertices are colored according to the type of interface they belong to (cell-cell interfaces are blue, while apical and basal interfaces are orange and yellow, respectively), and each cell contains a nucleus tagged according to the cell number. Periodic boundary conditions are used in the x-axis. Both axes are taken to be relative to the characteristic length scale 𝐿0=√𝐴0. B. To study the stability of a simple cell layer we consider an infinite tissue made up of alternating, identical wedge-shaped cells. The shape of one of these cells may be determined by its height (ℎ) and its basal width (𝑏𝑏), assuming that the apical and basal interfaces correspond to circular arcs and that cell-cell interfaces are straight. The width of any two adjacent cells adds up to double the average cell width 𝑏, which is used to characterize cellular density. Different initial conditions and relative tension parameters lead to different outcomes in the simultated pseudotstratified epithelia.
fzolessi.bsky.social
Apical cell extrusion could be due either to an induction of cell death, folowed by expulsion, or by live cell extrusion, a response to epithelial overcrowding. The experiments in the present report indicate the latter to be responsible for PMA effect, hence: mechanical forces must be involved!
Caspase inhibitor QVD-OPh treatment of chick neurulas did not affect normal morphology of the neuroepithelium, or the occurrence of apical cell extrusion upon PMA treatment. QVD-OPh treatmen effectively reduced apoptotoc cell death, without preventing apical cell extrusion in neural plate cells of chick embryos.
fzolessi.bsky.social
In @gaparicio.bsky.social previous work, we showed that knock-down of the actin-modulating protein MARCKS, or its phosphorylation (by activating PKC with PMA), caused neural tube closure failure, and loss of apico-basal polarity with apical cell extrusion in the chick embryo. doi.org/10.1002/dvg....
fzolessi.bsky.social
Preprint alert! Just from the oven, the adventures of a young theoretical biophysicist (Santi Bosch) in a usually strictly wet lab (BCDN): how is it that extreme forces in amniotes primary neurulation do not break the highly dynamic neuroepithelium apart?
www.biorxiv.org/content/10.1...
Mechanical conditions preventing live cell extrusion during primary neurulation in amniotes
During primary neurulation in amniote embryos, the neural plate gives rise to the neural tube in a process requiring the coordination of forces at different scales throughout a geometrically complex t...
www.biorxiv.org
fzolessi.bsky.social
A note to Uruguayans: you can have access to all the articles in the Special Issue from Timbó, timbo.org.uy
Buscador Timbó Foco
timbo.org.uy
fzolessi.bsky.social
IBRO gathers more than 90 neuroscience societies all around the globe, supporting with grants the efforts of researchers and students who push the boundaries of knowledge in this relevant area. These funds largely come from these two journals. Publish in @ibrojournals.bsky.social!!
fzolessi.bsky.social
We are also thankful to the whole team at Neuroscience @ibrojournals.bsky.social @elsevierconnect.bsky.social, whose professionalism made this VSI a reality. Neuroscience and Neuroscience Reports are published by the International Brain Research Organization @ibroorg.bsky.social. What is IBRO?
fzolessi.bsky.social
The guest editors, A. Silva, N. Vitureira and myself thank the 53 authors that wrote the 15 articles on cutting-edge research connecting Uruguay with the world. A tribute to the founders of @socneurouy.bsky.social, but also to the 100 years of neuroscience history in Uruguay.
fzolessi.bsky.social
The Virtual Special Issue of Neuroscience celebrating the 30th anniversary of @socneurouy.bsky.social is completed, and with a cover! Find it and read it in full here: sciencedirect.com/special-issu...
@ibroorg.bsky.social @ibrojournals.bsky.social
fzolessi.bsky.social
Mañana viernes!!
bsky.app/profile/fzol...
fzolessi.bsky.social
El próximo viernes 25 de julio, a las 10 AM, en el Salón de Seminarios 1, de Facultad de Ciencias, Gonzalo Aparicio hará la defensa pública de su Tesis de Doctorado (PEDECIBA Biología). Los esperamos!
fzolessi.bsky.social
El próximo viernes 25 de julio, a las 10 AM, en el Salón de Seminarios 1, de Facultad de Ciencias, Gonzalo Aparicio hará la defensa pública de su Tesis de Doctorado (PEDECIBA Biología). Los esperamos!
Reposted by Flavio Zolessi
blockintheback.bsky.social
🚨🚨🚨 Tol2kit announcement! We have rebuilt the wiki that was corrupted a few months ago! We’re in the process of also linking it to the original address. Please visit us here:
tol2kitkwan.genetics.utah.edu
Thank you for your patience!!
#zebrafish #transgenesis #plasmids #sharing
tol2kit for kwan lab
tol2kitkwan.genetics.utah.edu
fzolessi.bsky.social
Happy to share the last article of the Virtual Special Issue of Neuroscience @ibrojournals.bsky.social devoted to the 30th anniversary of @socneurouy.bsky.social: the editorial! Coauthorship shared with Ana Silva and Nathalia Vitureira. authors.elsevier.com/a/1lL%7Eq_6z...
@ibroorg.bsky.social
Reposted by Flavio Zolessi
ibroorg.bsky.social
🚨 Call for proposals! We’re putting together the #IBRO2027 Scientific Program Committee—and we want your input! Do you know someone who would bring valuable perspective & expertise? Submit your proposals by 30 May: buff.ly/kD3n8nf

#conference #neuroscience @thebalelab.bsky.social