Advances in sequencing technologies have enabled transcriptional profiling of previously understudied yet critical species, such as mosquitoes. Here, we present a protocol for multiplex whole-mount RNA fluorescence in situ hybridization combined with immunohistochemistry in the Anopheles gambiae brain. We describe steps for dissection, fixation, probe hybridization, hybridization chain reaction (HCR) probe detection, and primary antibody application. We then detail procedures for tissue preparat...

Molecular evolution studies often rely on single-copy orthologs. This study presents OrthoSNAP, an algorithm that identifies and extracts additional single-copy orthologs nested within larger gene fam...

Data and code are essential for ensuring the credibility of scientific results and facilitating reproducibility, areas in which journal sharing policies play a crucial role. However, in ecology and ev...

Abstract. Sensory adaptation is widely hypothesized to drive ecological speciation, yet empirical evidence from natural populations undergoing early stage

Background Variation within the prokaryotic pangenome is not random, and natural selection that favours particular combinations of genes appears to dominate over random drift. What is less clear is wh...

Single-cell RNA-sequencing revolutionized our approach of transcriptomic studies, enabling to analyze gene expression across cell type in a tissue. Here we introduce an optimized cell dissociation and...

Communication is crucial to social life, and in ants, it is mediated primarily through olfaction. Ants have more odorant receptor (OR) genes than any other group of insects, generated through tandem d...

Abstract. Collective behavior forms the basis for many antipredator strategies. Within Lepidoptera, larval gregariousness has evolved convergently across m

Lepidoptera, having co-diversified with flowering plants and adapted to various diel niches, present a remarkable system for studying compound eye cell type diversity. Here we synthesize the latest re...

Toutes nos félicitations à Violaine Llaurens (DR CNRS, CIRB & ISYEB, Paris & Université des Antilles), lauréate du Prix Recherche SFE² 2025.