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Reposted by Laboratory of Chemical and Biological Probes

Department of Chemistry | University of Zurich @chem.uzh.ch · 3d

The @locbp.bsky.social @chem.uzh.ch developed a gene expression reporter system based on the coexpression of the protein of interest BiP/GRP78 and the reporter HaloTag. chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...

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Laboratory of Chemical and Biological Probes @locbp.bsky.social · 26d

Congratulations to Hen (again)! Her paper, in collaboration with Jade, Juan, and the EPFL ACCESS team was just published. The paper describes a chemigenetic gene reporter tool for time-stamping and pulse-chase-type experiments.
chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...

Two‐Color Timestamping of Gene Expression with a Chemigenetic Reporter System

A chemigenetic reporter assay is developed that enables monitoring of BiP/GRP78 expression, an important chaperone and key regulator of the unfolded protein response. The system is based on the coexp....

chemistry-europe.onlinelibrary.wiley.com

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Laboratory of Chemical and Biological Probes @locbp.bsky.social · 29d

Congratulation's to Hen! Great job presenting her chemigenetic gene expression reporter system!

Department of Chemistry | University of Zurich @chem.uzh.ch · Sep 9

Congratulations to all the Poster Presentation Awardees @chem.uzh.ch at the SCS Fall Meeting: Noah Rychener 👏, Vincent Freiburghaus 👏, Henriette Lämmermann 👏, Fan Liu 👏, Nanchen Dongfang 👏 and Shangkun Li 👏

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Laboratory of Chemical and Biological Probes @locbp.bsky.social · Aug 26

Congrats to Juan! His discovery of hemicyanines as potent activators of paraptotic cell death has just been published! With great contributions from Jade (initial observations of ER swelling) and Craig (crucial supporting experiments)!
pubs.acs.org/doi/10.1021/...

Potent Inducers of Paraptosis through Electronic Tuning of Hemicyanine Electrophiles

Paraptosis is a distinct form of programmed cell death characterized by cytoplasmic vacuolization, mitochondrial swelling, and endoplasmic reticulum (ER) dilation, offering an alternative to apoptosis...

pubs.acs.org

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Laboratory of Chemical and Biological Probes @locbp.bsky.social · Apr 30

Happy to share the results of a fun side project in which we used the COUPY dye and mutants of HaloTag7 to show that changes in fluorescence lifetime correlate with the polarity of the protein binding pocket!
chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1...
#FLIM #fluorescence #HaloTag

Fluorescence Lifetime Multiplexing with Environment‐Sensitive Chemigenetic Probes

HaloTag (HT) is a versatile self-labeling protein that has been widely adopted in fluorescence microscopy. Besides its established use as an intensity-based marker and sensor, interest in using HT fo....

chemistry-europe.onlinelibrary.wiley.com

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Laboratory of Chemical and Biological Probes @locbp.bsky.social · Apr 7

🚨Open PhD positions in chemical biology!🚨

Join us in developing chemigenetic probes for imaging and sensing.

⚗️🧫Experience in chemical synthesis and/or protein engineering?

🗻🇨🇭Love mountains, chocolate, and a safe, diverse, and engaging environment?

Apply (or re-post)!
jobs.uzh.ch/job-vacancie...

UZH: PhD position in Chemical Biology

In the Laboratory of Chemical and Biological probes (PI: Pablo Rivera Fuentes), we do research at the interface of chemistry and biology, particularly in the fields of redox biology, development of im...

jobs.uzh.ch

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Reposted by Laboratory of Chemical and Biological Probes

Deliz Liang lab @lab-adl.bsky.social · Mar 17

🚨 PhD Opportunity!

Passionate about enzyme engineering or genetic code expansion? 🧬 Our lab is hiring a PhD student!

🔬 Exciting interdisciplinary research
🤝 Engaging environment 🌱

📄 Does this sound like you, apply now! ⏳🔗
📢 Know someone? Share this! 🔄

jobs.uzh.ch/job-vacancie...

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Reposted by Laboratory of Chemical and Biological Probes

Peter Stacko @pstacko.bsky.social · Feb 25

Looking for a PhD candidate to join my group at the University of Zurich If you are an excellent and motivated student🧑‍🔬and want to integrate organic synthesis, photochemistry and redox chemistry in a research🧪 we want to know more about you! More info: www.stacko.group Appreciate sharing the post.

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Laboratory of Chemical and Biological Probes @locbp.bsky.social · Feb 20

Now online at a peer-reviewed journal, Carla's careful characterization of the redox state of glutathione in the Golgi apparatus: www.sciencedirect.com/science/arti...

Characterization of the Glutathione Redox State in the Golgi Apparatus

Redox homeostasis is crucial for cell function, and, in eukaryotic cells, studying it in a compartmentalized way is essential due to the redox variati…

www.sciencedirect.com

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Reposted by Laboratory of Chemical and Biological Probes

Deliz Liang lab @lab-adl.bsky.social · Feb 11

Exciting news from the lab! Our first preprint, “Hydrophobic Tuning with Non-Canonical Amino Acids in a Copper Metalloenzyme” is now available.
In this study, we rewrite the rules of enzyme engineering with a cyclic, hydrophobic twist that’s anything but ordinary.
🔍: www.biorxiv.org/content/10.1...

Hydrophobic tuning with non-canonical amino acids in a copper metalloenzyme

Hydrophobicity controls many aspects of protein and enzyme function. Although hydrophobic tuning can be achieved to a limited extent with canonical amino acids, the incorporation of non- canonical ami...

www.biorxiv.org

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Laboratory of Chemical and Biological Probes @locbp.bsky.social · Jan 27

Congratulations to @puentene.bsky.social, who won the best poster award at #SMPS24 @smps4.bsky.social!!

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Reposted by Laboratory of Chemical and Biological Probes

SMPS4 @smps4.bsky.social · Jan 23

Another brilliant session by Pablo Rivera-Fuentes from the University of Zurich on 'Single-Molecule Protein Identification using Blinking Patterns.'
#SMPS4

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Laboratory of Chemical and Biological Probes @locbp.bsky.social · Dec 9

Just out on bioRxiv, Carla Miró reports the first quantitative determination of the redox state of glutathione in the Golgi apparatus. Spoiler alert: it is very oxidized and in relatively low concentrations!
www.biorxiv.org/content/10.1...

Characterization of the Glutathione Redox State in the Golgi Apparatus

Redox homeostasis is crucial for cell function, and, in eukaryotic cells, studying it in a compartmentalized way is essential due to the redox variations between different organelles. The redox state ...

www.biorxiv.org

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Laboratory of Chemical and Biological Probes @locbp.bsky.social · Nov 16

👋

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Laboratory of Chemical and Biological Probes @locbp.bsky.social · Nov 13

Long overdue, we have left the propaganda machine formerly known as Twitter and will be posting exclusively here!

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