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Lucy Dornan @lucygdornan.bsky.social · 4h

Sorry to hear! Did you do mutagenesis to rectify it or have you just been working with and acknowledging the cytoplasmic pool? Trying to understand how many of my experiments need repeating over the next few months 🥹

Lucy Dornan @lucygdornan.bsky.social · 4h

i can only pray that anybody else who needs to learn this lesson is not learning it 11 months out from submitting

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Lucy Dornan @lucygdornan.bsky.social · 5h

After many years of hm-ing and haw-ing about why some of the fusion proteins I’ve cloned showed cytoplasmic background I’ve finally found the culprit

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Lucy Dornan @lucygdornan.bsky.social · 12d

Spending my weekend churning out data from live cell time series for a poster I have left very, very last minute; but at least the backdrop is pretty 🥹💞

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Lucy Dornan @lucygdornan.bsky.social · 15d

Late night live cell sesh, captured by @madeleenbrink.bsky.social 🫶

(featuring hair tied back by the chopped off end of a latex glove, life hack for all long hair havers who find themselves short of a hair tie in the lab)

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Lucy Dornan @lucygdornan.bsky.social · 15d

How does a cell just fit that much stuff inside of it . Like there is SO MUCH jammed into something SO small .

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Lucy Dornan @lucygdornan.bsky.social · 28d

Ssssssshhhhh if we don’t think about the data analysis it can’t hurt us

Lucy Dornan @lucygdornan.bsky.social · 28d

Just finished imaging my last rep of an experiment that has taken a grand total of 6100 minutes (101.66 hrs) of live-cell confocal imaging, spread across 305x 35mm dishes

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Lucy Dornan @lucygdornan.bsky.social · Sep 4

Went outside to go look at the stars and all I can see are Starlink satellites . :(

Lucy Dornan @lucygdornan.bsky.social · Aug 23

Go Kylie go!!!!

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Reposted by Lucy Dornan

Fran Bottanelli @franbottanelli.bsky.social · Aug 20

If you have an exciting microscopy-based membrane biology discovery to showcase this may be for you! Submit your abstracts and/or get in touch! Please repost

FocalPlane @focalplane.bsky.social · Aug 20

Last call for applications for ECRs to speak at our #FocalPlaneFeatures symposium co-hosted with the #MembraneTrafficking webinar series organised by @franbottanelli.bsky.social @felixmendu.bsky.social & @ishier.bsky.social.

Apply by 24 August
focalplane.biologists.com/2025/07/14/f...

FocalPlane features... membrane trafficking - apply to give a talk - FocalPlane

FocalPlane features... membrane trafficking - apply to give a talk - News

focalplane.biologists.com

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Lucy Dornan @lucygdornan.bsky.social · Aug 15

A brief moment of solitude and reflection beneath the trees today :)

After 7 years in UCD between my undergrad and the PhD, today I finished my last ever accredited module. Feeling so grateful for the opportunity to have learnt so much about myself and the world, and excited for the future :)

Reposted by Lucy Dornan

Madeleen Brink @madeleenbrink.bsky.social · Aug 14

Taking a shot here, a colleague is seeking a #postdoc position in/near #Germany. She’s eager to expand into #organoids , & has experience in #Spheroids, #BiologicalImaging, #CellBiology #HighContentMicroscopy
Any leads, connections, or suggestions would be hugely appreciated!
#Microscopy

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Reposted by Lucy Dornan

Johannes Morstein @morstein.bsky.social · Aug 14

Excited to share our new @pubs.acs.org paper! We engineered cells with ~10% photolipids in the ER membrane. This enabled optical control of membrane viscosity to study its impact on ER→Golgi protein transport. @dirktrauner.bsky.social @noemijimenezrojo.bsky.social

pubs.acs.org/doi/10.1021/...

Optical Control of Membrane Viscosity Modulates ER-to-Golgi Trafficking

The lipid composition of cellular membranes is highly dynamic and undergoes continuous remodeling, affecting the biophysical properties critical to biological function. Here, we introduce an optical a...

pubs.acs.org

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Lucy Dornan @lucygdornan.bsky.social · Aug 14

Just out of curiosity… who here also maintains an active LinkedIn profile?

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Reposted by Lucy Dornan

Fran Bottanelli @franbottanelli.bsky.social · Aug 13

Our protocol for gene editing is online and all plasmids are on @addgene.bsky.social! CRISPR away everybody and get in touch if you need anything 🤩

STAR Protocols @cp-starprotocols.bsky.social · Aug 7

Protocol for fast antibiotic resistance-based gene editing of mammalian cells with CRISPR-Cas9 #protocol #starprotocols #cellpress

Protocol for fast antibiotic resistance-based gene editing of mammalian cells with CRISPR-Cas9

Protein tagging with CRISPR-Cas9 enables the investigation of protein function in its native environment but is limited by low homology-directed repair (HDR) efficiency. Here, we present a protocol for fast antibiotic resistance-based gene editing with CRISPR-Cas9 (FAB-CRISPR), which streamlines N/C-terminal tagging using an antibiotic resistance cassette for rapid selection and enrichment of gene-edited cells. We describe in detail guide RNA and HDR donor plasmid cloning, transfection of editin...

dlvr.it

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Lucy Dornan @lucygdornan.bsky.social · Aug 12

(today is a head in hands day)

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Lucy Dornan @lucygdornan.bsky.social · Aug 12

Going through a lot of flip flopping between psyching and hyping myself up to really knuckle tf down for final year, and then just staring at my to-do list and calendar like this

a woman with curly hair is sitting at a desk with her hands on her head and a hulu logo in the corner

ALT: a woman with curly hair is sitting at a desk with her hands on her head and a hulu logo in the corner

media.tenor.com

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Lucy Dornan @lucygdornan.bsky.social · Aug 9

Innovation 😎

Lucy Dornan @lucygdornan.bsky.social · Aug 9

Label-free, I don’t know enough on optics to comment on the other options you’ve given 🥴 it’s entirely a pipe dream, I just want to put them on & feel like I’m watching one of those animated videos of intracellular molecules in 4D when I look at my dish of cells (e.g. youtu.be/Jr5KEpBj_KQ?...)

Protein Packing Inside the Cell (BioVisions) (Official Version)

YouTube video by LabXchange

youtu.be

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Lucy Dornan @lucygdornan.bsky.social · Aug 9

Exactly exactly exactly, now we’re onto something!!

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Lucy Dornan @lucygdornan.bsky.social · Aug 8

Need money :(

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Lucy Dornan @lucygdornan.bsky.social · Aug 8

Like what do you MEAN I can only look at a handful in isolation out of thousands of molecules at any one time ???? Sounds and feels like a scam personally

Lucy Dornan @lucygdornan.bsky.social · Aug 8

Personally I think that scientists should invent a pair of goggles that lets you see every single molecular interaction in a single cell at once

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