This protocol for the use and quality control of genetically encoded reporters to label proteins and cells in correlative light and electron microscopy workflows enables the acquisition of molecular, ...

The metric tools that aim to assess greenness, whiteness or related aspects of analytical procedures are gaining in popularity. At the same time, thei…

GreenSOL is the first comprehensive solvent selection guide tailored specifically to analytical chemistry. It employs a life cycle approach to evaluat…

Nature Protocols, Published online: 19 November 2025; doi:10.1038/s41596-025-01272-3This Protocol Extension describes directional conjugation of monoclonal antibodies to nanoparticles via the Fc antibody moiety using metal-free click chemistry, including procedures for synthesis, quality control and evaluation of nanoparticle–antibody conjugates.

Nature Protocols, Published online: 19 November 2025; doi:10.1038/s41596-025-01260-7This protocol for the use and quality control of genetically encoded reporters to label proteins and cells in correlative light and electron microscopy workflows enables the acquisition of molecular, cellular and biochemical information at high resolution.

Nature Protocols, Published online: 19 November 2025; doi:10.1038/s41596-025-01282-1This robust, proteome-wide approach for the exploration of the S-nitrosoproteome in human and mouse tissues uses the SNOTRAP probe and nano-liquid chromatography–tandem mass spectrometry analysis. The protocol enables efficient and high-throughput profiling of S-nitrosylated proteins in complex mixtures of biological material.

Nature Protocols, Published online: 19 November 2025; doi:10.1038/s41596-025-01271-4BreakTag is a scalable next-generation sequencing-based method for the unbiased characterization of programmable nucleases and guide RNAs that allows off-target and nuclease activity assessment, as well as the characterization of scission profiles.

Nature Protocols, Published online: 18 November 2025; doi:10.1038/s41596-025-01273-2Reproducible characterization of materials designed for radiative cooling is important for their development and application. The protocol describes how to acquire optical data and how to build and use outdoor and indoor thermal testing platforms.

Nature Protocols, Published online: 14 November 2025; doi:10.1038/s41596-025-01276-zThis Protocol describes preprocessing of different environmental samples and extraction of microplastics and nanoplastics, as well as qualitative and quantitative characterization of microplastics and nanoplastics. The analytical technologies used for characterization include attenuated total reflection-Fourier transform infrared spectroscopy, laser direct infrared spectroscopy and optical photothermal infrared microspectroscopy.

Job Title: Locum Associate or Senior Editor, Nature Reviews Locations: Heidelberg or New York - hybrid working model Application Deadline: 26th November 2025 – candidates will be considered as they ap...

Nature Protocols, Published online: 11 November 2025; doi:10.1038/s41596-025-01283-0This protocol describes the synthesis of macrocyclic macroPROTAC1 and trivalent PROTAC SIM1, detailing the generation of the macrocyclic and trivalent cores and their conjugation to the respective ligands, for enhanced target protein degradation.

Clear methods reporting is key for reliable and reproducible science and can also prevent an extended review process. We highlight Methods section requirements for a more efficient publication process...

Communications AI & Computing will be a broad journal covering applied computer science with a focus on the development of artificial intelligence methods and ...

Nature Protocols, Published online: 30 October 2025; doi:10.1038/s41596-025-01268-zWe provide a protocol for generating human pluripotent stem cell-derived blood-generating heart-forming organoids, a multitissue model encompassing aspects of human cardiac, endothelial and hematopoietic co-development, and techniques to stain and clear these large samples for laser microscopy.

The NFDI4Chem supports chemists to collect, store, process, publish and re-use research data, so that their data meet the FAIR conditions.

Nature Protocols, Published online: 28 October 2025; doi:10.1038/s41596-025-01253-6Slippery covalently attached liquid surfaces are useful in many applications that require low-static friction to droplets. This review compares six prominent methods for preparing polydimethylsiloxane bound to silica surfaces and highlights pitfalls and time savers.

Nature Protocols, Published online: 16 October 2025; doi:10.1038/s41596-025-01257-2ANPELA is a software package to compare and assess the performance of different workflows for processing single-cell proteomic data, ensuring the user selects the most appropriate processing workflow for their experimental design question.

Nature Protocols, Published online: 15 October 2025; doi:10.1038/s41596-025-01258-1The preparation of multicomponent viscoelastic self-enhancing sono-inks, synthesized as phase-transition reversible acoustic absorbers, enables acoustic volumetric printing beneath diverse tissue types in optically scattering media.

Nature Protocols, Published online: 13 October 2025; doi:10.1038/s41596-025-01255-4In mass photometry, the optical contrast generated by individual molecules at a glass–water interface enables mass-resolved quantification of biomolecular mixtures. This protocol describes how to optimize and validate this method.

Effectively engaging with large language models is becoming increasingly vital as they proliferate across research landscapes. This Comment presents a practical guide for understanding their capabilities and limitations, along with strategies for crafting well-structured queries, to extract maximum utility from these artificial intelligence tools.