Nature Protocols
@natprot.nature.com
New Article! Single-nucleus chromatin accessibility and gene expression co-profiling by ISSAAC-seq
Single-nucleus chromatin accessibility and gene expression co-profiling by ISSAAC-seq
Nature Protocols, Published online: 12 January 2026; doi:10.1038/s41596-025-01304-yThis protocol describes a flexible workflow for joint profiling of chromatin accessibility and transcriptomes in single nuclei, compatible with either plate or droplet single-cell isolation platforms.
dlvr.it
January 12, 2026 at 10:48 AM
New Article! Single-nucleus chromatin accessibility and gene expression co-profiling by ISSAAC-seq
New Article! Hydrogen/deuterium exchange mass spectrometry analysis of ribosome-nascent chain complexes to study protein biogenesis at the peptide level
Hydrogen/deuterium exchange mass spectrometry analysis of ribosome-nascent chain complexes to study protein biogenesis at the peptide level
Nature Protocols, Published online: 12 January 2026; doi:10.1038/s41596-025-01279-wA strategy for isolating Escherichia coli ribosome–nascent chain complexes and analyzing their conformational dynamics and interactors at peptide level using hydrogen–deuterium exchange mass spectrometry is presented.
dlvr.it
January 12, 2026 at 10:48 AM
New Article! Hydrogen/deuterium exchange mass spectrometry analysis of ribosome-nascent chain complexes to study protein biogenesis at the peptide level
New Article! Deployment of a cloud-based passive defecation monitoring system for continuous gut health monitoring
Deployment of a cloud-based passive defecation monitoring system for continuous gut health monitoring
Nature Protocols, Published online: 09 January 2026; doi:10.1038/s41596-025-01296-9In this protocol, the authors explain how to set up and use the cloud-based Precision Health Integrated Diagnostic (PHIND) system, a smart-toilet-based platform for passive and automated defecation monitoring to provide continuous gut health information.
dlvr.it
January 10, 2026 at 9:30 AM
New Article! Deployment of a cloud-based passive defecation monitoring system for continuous gut health monitoring
New Article! Acoustic separation and isolation of viruses, small extracellular vesicles and other nanoscale bioparticles
Acoustic separation and isolation of viruses, small extracellular vesicles and other nanoscale bioparticles
Nature Protocols, Published online: 09 January 2026; doi:10.1038/s41596-025-01286-xThis protocol covers the acoustics-based separation of biological particles such as viruses and single extracellular vesicles from biofluids, including plasma and saliva, with high purity.
dlvr.it
January 10, 2026 at 9:30 AM
New Article! Acoustic separation and isolation of viruses, small extracellular vesicles and other nanoscale bioparticles
New Article! In vivo Raman spectroscopy for real-time biochemical assessment of tissue pathology and physiology
In vivo Raman spectroscopy for real-time biochemical assessment of tissue pathology and physiology
Nature Protocols, Published online: 07 January 2026; doi:10.1038/s41596-025-01274-1We present a protocol for the acquisition and analysis of in vivo Raman spectra to extract biochemical information from tissues for biomedical applications.
bit.ly
January 7, 2026 at 9:04 AM
New Article! In vivo Raman spectroscopy for real-time biochemical assessment of tissue pathology and physiology
Take a look at Nature Methods December issue for 8 methods to watch in 2026! Virtual cells, the cancer microbiome, protein editing, and more... www.nature.com/nmeth/volume...
Nature Methods - Method of the Year 2025: EM-based connectomics
Auditory neurons in the FlyWire/FAFB whole-brain connectome.See Editorial
www.nature.com
January 6, 2026 at 6:53 PM
Take a look at Nature Methods December issue for 8 methods to watch in 2026! Virtual cells, the cancer microbiome, protein editing, and more... www.nature.com/nmeth/volume...
Reposted by Nature Protocols
Our Method of the Year 2025 is...drumroll please...EM-based connectomics!!
Our Editorial introduces our choice and highlights six Comments and other related content in this special issue. Please join us in celebrating EM-based connectomics! 🎉🧠🔬
www.nature.com/articles/s41...
Our Editorial introduces our choice and highlights six Comments and other related content in this special issue. Please join us in celebrating EM-based connectomics! 🎉🧠🔬
www.nature.com/articles/s41...
Method of the Year 2025: electron microscopy-based connectomics - Nature Methods
A large network of interconnected neurons serves as the basis of brain function and of behavior. Methodological advances have enabled the reconstruction of large-scale and even whole-brain connectomes...
www.nature.com
December 8, 2025 at 4:59 PM
Our Method of the Year 2025 is...drumroll please...EM-based connectomics!!
Our Editorial introduces our choice and highlights six Comments and other related content in this special issue. Please join us in celebrating EM-based connectomics! 🎉🧠🔬
www.nature.com/articles/s41...
Our Editorial introduces our choice and highlights six Comments and other related content in this special issue. Please join us in celebrating EM-based connectomics! 🎉🧠🔬
www.nature.com/articles/s41...
Join Springer Nature editors and the protocols.io team for two exciting events at #LoveMethods26!
💻Transform your protocols at our Hackathon - Jan 21
🔮Gain insight on methods publishing at our Methods Mythbusting - Jan 22
👉FREE registration & more details: excelscior.uc.pt/love-methods...
💻Transform your protocols at our Hackathon - Jan 21
🔮Gain insight on methods publishing at our Methods Mythbusting - Jan 22
👉FREE registration & more details: excelscior.uc.pt/love-methods...
Bring structure to your research - protocols.io
A secure platform for developing and sharing reproducible methods.
protocols.io
January 6, 2026 at 2:11 PM
Join Springer Nature editors and the protocols.io team for two exciting events at #LoveMethods26!
💻Transform your protocols at our Hackathon - Jan 21
🔮Gain insight on methods publishing at our Methods Mythbusting - Jan 22
👉FREE registration & more details: excelscior.uc.pt/love-methods...
💻Transform your protocols at our Hackathon - Jan 21
🔮Gain insight on methods publishing at our Methods Mythbusting - Jan 22
👉FREE registration & more details: excelscior.uc.pt/love-methods...
New Article! CRISPR screens in human neural organoids and assembloids
CRISPR screens in human neural organoids and assembloids
Nature Protocols, Published online: 19 December 2025; doi:10.1038/s41596-025-01299-6This protocol combines pooled CRISPR–Cas9 screening with neural organoid and assembloid models and illustrates how it can be applied to map hundreds of disease genes onto cellular pathways and specific aspects of human neural development.
bit.ly
December 19, 2025 at 9:18 PM
New Article! CRISPR screens in human neural organoids and assembloids
#IgNobel face-to-face with Marc Abrahams (improbable.com/about/people...)
presented at #FallingWalls #ScienceSummit25
features a favourite IgNobel winner, Kees Moeliker and his duck.
www.youtube.com/watch?v=I1vP...
presented at #FallingWalls #ScienceSummit25
features a favourite IgNobel winner, Kees Moeliker and his duck.
www.youtube.com/watch?v=I1vP...
Ig Nobel Face-to-Face | Science Summit 2025
YouTube video by Falling Walls Foundation
www.youtube.com
December 19, 2025 at 5:16 PM
#IgNobel face-to-face with Marc Abrahams (improbable.com/about/people...)
presented at #FallingWalls #ScienceSummit25
features a favourite IgNobel winner, Kees Moeliker and his duck.
www.youtube.com/watch?v=I1vP...
presented at #FallingWalls #ScienceSummit25
features a favourite IgNobel winner, Kees Moeliker and his duck.
www.youtube.com/watch?v=I1vP...
Join us for “Methods Mythbusting: Insights from Journal Editors and protocols.io”protocols.io/webinars/met...rs
at Love Methods Week 2026excelscior.uc.pt/love-methods...6/#LoveMethods2626
at Love Methods Week 2026excelscior.uc.pt/love-methods...6/#LoveMethods2626
Methods Mythbusting: Insights from Journal Editors and protocols.io
This webinar will be held at: 3pm CET / 2pm GMT / 9am EST / 6am PST Join us for a lively session where we tackle common misconceptions about protocols and methods publishing. H...
protocols.io
December 15, 2025 at 8:18 AM
Join us for “Methods Mythbusting: Insights from Journal Editors and protocols.io”protocols.io/webinars/met...rs
at Love Methods Week 2026excelscior.uc.pt/love-methods...6/#LoveMethods2626
at Love Methods Week 2026excelscior.uc.pt/love-methods...6/#LoveMethods2626
From Nature: 23 December 1869 - a news article about Mistletoe
"When the leaves are rotting on the ground, and the fruit has been converted into cider..."
www.nature.com/articles/001...
"When the leaves are rotting on the ground, and the fruit has been converted into cider..."
www.nature.com/articles/001...
Mistletoe - Nature
Nature - Mistletoe
www.nature.com
December 12, 2025 at 7:50 AM
From Nature: 23 December 1869 - a news article about Mistletoe
"When the leaves are rotting on the ground, and the fruit has been converted into cider..."
www.nature.com/articles/001...
"When the leaves are rotting on the ground, and the fruit has been converted into cider..."
www.nature.com/articles/001...
An essay on the history of the RI Christmas lectures published in nature last week.
www.nature.com/articles/d41...
www.nature.com/articles/d41...
How the Royal Institution made science a seasonal spectacle
From flaming raisins to robots and rockets, the institution’s Christmas Lectures have educated and entertained for two centuries.
www.nature.com
December 11, 2025 at 2:32 PM
An essay on the history of the RI Christmas lectures published in nature last week.
www.nature.com/articles/d41...
www.nature.com/articles/d41...
The Royal Institution of Science's Christmas lectures start today!
www.rigb.org/christmas-le...
www.rigb.org/christmas-le...
2025 CHRISTMAS LECTURES livestreaming
Find out where across the country you can watch a livestreaming of the 2025 CHRISTMAS LECTURES.
www.rigb.org
December 11, 2025 at 9:40 AM
The Royal Institution of Science's Christmas lectures start today!
www.rigb.org/christmas-le...
www.rigb.org/christmas-le...
New Article! Visualizing infection by single positive-sense RNA viruses using virus infection real-time imaging (VIRIM)
Visualizing infection by single positive-sense RNA viruses using virus infection real-time imaging (VIRIM)
Nature Protocols, Published online: 04 December 2025; doi:10.1038/s41596-025-01290-1Virus infection real-time imaging visualizes viral RNA translation within cells in real-time, revealing the earliest events of viral infection when viral protein and RNA levels are low, as well as the origins and consequences of cell-to-cell heterogeneity during virus infection.
bit.ly
December 5, 2025 at 12:46 AM
New Article! Visualizing infection by single positive-sense RNA viruses using virus infection real-time imaging (VIRIM)
New Article! Using tunable hydrogel microparticles to measure cellular forces
Using tunable hydrogel microparticles to measure cellular forces
Nature Protocols, Published online: 04 December 2025; doi:10.1038/s41596-025-01281-2Deformable poly-acrylamide co-acrylic acid microparticles (DAAM-particles) are tunable hydrogel microparticles for quantifying cellular forces. This comprehensive protocol details their synthesis, functionalization and applications.
bit.ly
December 5, 2025 at 12:46 AM
New Article! Using tunable hydrogel microparticles to measure cellular forces
New Article! Bioengineering mini-colons for ex vivo colorectal cancer research
Bioengineering mini-colons for ex vivo colorectal cancer research
Nature Protocols, Published online: 03 December 2025; doi:10.1038/s41596-025-01292-zThis protocol integrates state-of-the-art microfabrication, tissue engineering and optogenetic approaches to generate topobiologically complex miniature colons capable of undergoing tumorigenesis in vitro.
bit.ly
December 4, 2025 at 12:36 AM
New Article! Bioengineering mini-colons for ex vivo colorectal cancer research
In another inspiring talk at #EMBOComp3D, Julia Mahamid spoke about recent in-cell cryo-ET results
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
In-cell discovery and characterization of a non-canonical bacterial protein translocation-folding complex
Cryo-electron tomography has emerged as powerful technology for in-cell structural biology, and in combination with breakthroughs in protein structure prediction, offers a unique opportunity for illum...
www.biorxiv.org
December 3, 2025 at 4:03 PM
In another inspiring talk at #EMBOComp3D, Julia Mahamid spoke about recent in-cell cryo-ET results
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
New Article! Testing, quantification, in situ characterization and calculation simulation for electrocatalytic nitrate reduction
Testing, quantification, in situ characterization and calculation simulation for electrocatalytic nitrate reduction
Nature Protocols, Published online: 02 December 2025; doi:10.1038/s41596-025-01289-8Rigorous characterization is important for developing electrocatalytic reactions, for example, nitrate reduction. This workflow integrates bulk and surface techniques with in situ/operando spectroscopies, electrochemical measurements and density functional theory calculations.
bit.ly
December 3, 2025 at 12:27 AM
New Article! Testing, quantification, in situ characterization and calculation simulation for electrocatalytic nitrate reduction
WEHI.TV explains discoveries at the frontier of medical research through accurate and entertaining 3D animation. www.wehi.edu.au/wehi-tv/
Drew Berry gave an inspiring talk at #EMBOComp3D
Drew Berry gave an inspiring talk at #EMBOComp3D
WEHI.TV Biomedical Animations | WEHI
WEHI.TV explains discoveries at the frontier of medical research through accurate and entertaining 3D animation.
www.wehi.edu.au
December 2, 2025 at 3:04 PM
WEHI.TV explains discoveries at the frontier of medical research through accurate and entertaining 3D animation. www.wehi.edu.au/wehi-tv/
Drew Berry gave an inspiring talk at #EMBOComp3D
Drew Berry gave an inspiring talk at #EMBOComp3D
New Article! Assaying the myosin super-relaxed state across muscle types, cells and proteins for understanding muscle biology and use in drug discovery
Assaying the myosin super-relaxed state across muscle types, cells and proteins for understanding muscle biology and use in drug discovery
Nature Protocols, Published online: 01 December 2025; doi:10.1038/s41596-025-01291-0The Mant-ATP assay is a simple and accessible means for rapid quantitative assessment of the ratio of super-relaxed myosin to disordered relaxed myosin. This protocol provides a standardized methodology to perform the assay across a range of systems.
bit.ly
December 2, 2025 at 12:19 AM
New Article! Assaying the myosin super-relaxed state across muscle types, cells and proteins for understanding muscle biology and use in drug discovery
New Article! XomicsToModel: omics data integration and generation of thermodynamically consistent metabolic models
XomicsToModel: omics data integration and generation of thermodynamically consistent metabolic models
Nature Protocols, Published online: 01 December 2025; doi:10.1038/s41596-025-01288-9This Protocol describes XomicsToModel, a semi-automated pipeline that integrates bibliomic, transcriptomic, proteomic and metabolomic data with a generic genome-scale metabolic reconstruction or model.
bit.ly
December 2, 2025 at 12:19 AM
New Article! XomicsToModel: omics data integration and generation of thermodynamically consistent metabolic models
New Article! Measuring cellular force using DNA-based tension probes: from ensemble to single-molecule studies
Measuring cellular force using DNA-based tension probes: from ensemble to single-molecule studies
Nature Protocols, Published online: 28 November 2025; doi:10.1038/s41596-025-01277-yThe authors present a protocol for the use of two advanced DNA-based tension probes, the reversible shearing DNA-based tension probe (RSDTP) and ForceChrono probe, which provide powerful tools for studying mechanotransduction in living cells.
bit.ly
November 29, 2025 at 1:56 AM
New Article! Measuring cellular force using DNA-based tension probes: from ensemble to single-molecule studies
Reposted by Nature Protocols
Welcome to Communications Health! We are a new, open-access journal from @natureportfolio.nature.com. Now open for submissions in public and global health!
www.nature.com/commshealth
#PublicHealth #GlobalHealth #OpenAccess
www.nature.com/commshealth
#PublicHealth #GlobalHealth #OpenAccess
Communications Health
Communications Health is an open access journal from Nature Portfolio publishing high-quality research, reviews and commentary in all population ...
www.nature.com
November 27, 2025 at 11:33 AM
Welcome to Communications Health! We are a new, open-access journal from @natureportfolio.nature.com. Now open for submissions in public and global health!
www.nature.com/commshealth
#PublicHealth #GlobalHealth #OpenAccess
www.nature.com/commshealth
#PublicHealth #GlobalHealth #OpenAccess
Reposted by Nature Protocols
🌟 Love Methods Week 2026 🌟
📅 19–23 January 2026 | 💻 Online
Join us for a full week of workshops & talks designed to boost the robustness, transparency, and reproducibility of your research methods!
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🔗 Learn more / Register 👇
📅 19–23 January 2026 | 💻 Online
Join us for a full week of workshops & talks designed to boost the robustness, transparency, and reproducibility of your research methods!
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Love Methods Week 2026 – Excelsior
excelscior.uc.pt
November 19, 2025 at 11:37 AM
🌟 Love Methods Week 2026 🌟
📅 19–23 January 2026 | 💻 Online
Join us for a full week of workshops & talks designed to boost the robustness, transparency, and reproducibility of your research methods!
#LoveMethods26
🔗 Learn more / Register 👇
📅 19–23 January 2026 | 💻 Online
Join us for a full week of workshops & talks designed to boost the robustness, transparency, and reproducibility of your research methods!
#LoveMethods26
🔗 Learn more / Register 👇