Something different from the lab!
We need better ALS biomarkers to minimise size, duration and cost of trials. Muscle MRI could work as it reliably detects progressive atrophy, and correlates with muscle strength loss.
@uclqsion.bsky.social @uclbrainscience.bsky.social
jnnp.bmj.com/content/earl...

x-axis is time after transformation - was re-transforming a plasmid for this test

PSA for anyone interested in growing E. coli for plasmid preps in 96 wells. (this is 1 ml of NEB Stable at 37C 240 RPM or 30C 180 RPM). Using TB enables you to obtain as much DNA as a standard miniprep, beautiful

If you tell me the sequence, I can almost certainly make it, even if my method is different

Can we make it a rule that when people publish papers involving generation of new plasmids, they put the plasmid sequence in the supplementary? Much more useful than "we digested plasmid A with enzyme B then ligated to C then digested with D then gel purified etc etc etc"

We're so pleased that groups have been ordering our TDP-REG vectors from Addgene! Please be aware that splicing minigenes cannot easily be used with lentiviruses, because the lentiviral RNA can get spliced before viral packaging! Better to use piggybac or similar, AAV, or just transient transfection

Glad I joined this platform so I don't miss good quality content like this