Richard Held
@rgsheld.bsky.social
Neuroscientist / synaptic physiologist interested in protein topography and cellular signal transduction. Ephys in the past, cryoET now, mouse genetics forever.
https://scholar.google.com/citations?hl=en&user=ESpBK1AAAAAJ
https://scholar.google.com/citations?hl=en&user=ESpBK1AAAAAJ
Pinned
Richard Held
@rgsheld.bsky.social
· Nov 13
Reintro: I'm a neuroscientist interested in how cells use molecular topography to control signal transduction. I use #CryoET and mouse genetic tools to map the topography of synaptic proteins and determine in-situ structures of scaffolding interactions that control said topography.
Set my deadlift PR for the year 🤫
January 2, 2026 at 2:26 AM
Set my deadlift PR for the year 🤫
June does christmas right
December 25, 2025 at 3:51 AM
June does christmas right
December 7, 2025 at 11:32 PM
Absolutely insane paragraph
December 7, 2025 at 2:49 AM
Absolutely insane paragraph
Reposted by Richard Held
The Nobel Prize committee should announce the World Cup winner tomorrow
December 6, 2025 at 4:29 AM
The Nobel Prize committee should announce the World Cup winner tomorrow
Reposted by Richard Held
Out today, we can now multiplex receptor mapping at synapses thanks to a dimeric gold nanoparticle label optimized by Hoyoung that's distinguishable from monomers!
Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography | PNAS www.pnas.org/doi/10.1073/...
Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography | PNAS www.pnas.org/doi/10.1073/...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
November 24, 2025 at 8:54 PM
Out today, we can now multiplex receptor mapping at synapses thanks to a dimeric gold nanoparticle label optimized by Hoyoung that's distinguishable from monomers!
Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography | PNAS www.pnas.org/doi/10.1073/...
Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography | PNAS www.pnas.org/doi/10.1073/...
I wrote a bit about practical considerations for using #cryo-ET labels and some things to work on if we want to find any protein in any cell.
authors.elsevier.com/a/1mA6h,LqAr...
authors.elsevier.com/a/1mA6h,LqAr...
authors.elsevier.com
December 1, 2025 at 3:43 PM
I wrote a bit about practical considerations for using #cryo-ET labels and some things to work on if we want to find any protein in any cell.
authors.elsevier.com/a/1mA6h,LqAr...
authors.elsevier.com/a/1mA6h,LqAr...
Reposted by Richard Held
ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms pubmed.ncbi.nlm.nih.gov/41315814/ #cryoem
November 30, 2025 at 11:30 PM
ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms pubmed.ncbi.nlm.nih.gov/41315814/ #cryoem
Not bad, not great
November 28, 2025 at 2:17 AM
Not bad, not great
Clearly a poorly resolved flexible domain here
November 18, 2025 at 12:40 AM
Clearly a poorly resolved flexible domain here
Finally got to go through this carefully. An interesting paper and a tremendous amount of work.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling
Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...
www.science.org
November 7, 2025 at 9:50 PM
Finally got to go through this carefully. An interesting paper and a tremendous amount of work.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Gonna need somebody to explain daylight savings to my dogs
November 3, 2025 at 2:11 AM
Gonna need somebody to explain daylight savings to my dogs
I think about this tweet on a near-daily basis
October 9, 2025 at 4:11 PM
I think about this tweet on a near-daily basis
This is what made America great
October 8, 2025 at 4:41 PM
This is what made America great
Reposted by Richard Held
The cat is officially out of the bag!
The LMB is home to a unique community of outstanding connectomics scientists — I'm so excited about adding my own molecular and expansion microscopy expertise to the mix!
This is going to be the most exciting journey of my scientific career — more soon! 🌈🧠
The LMB is home to a unique community of outstanding connectomics scientists — I'm so excited about adding my own molecular and expansion microscopy expertise to the mix!
This is going to be the most exciting journey of my scientific career — more soon! 🌈🧠
LMB welcomes @sventruckenbrodt.bsky.social as a new Group Leader in our Neurobiology Division!
Sven will develop molecular brain mapping, harnessing expansion microscopy & molecular biology methods to better understand the neural basis of behaviour.
www2.mrc-lmb.cam.ac.uk/the-lmb-welc... #LMBNews
Sven will develop molecular brain mapping, harnessing expansion microscopy & molecular biology methods to better understand the neural basis of behaviour.
www2.mrc-lmb.cam.ac.uk/the-lmb-welc... #LMBNews
September 15, 2025 at 9:54 AM
The cat is officially out of the bag!
The LMB is home to a unique community of outstanding connectomics scientists — I'm so excited about adding my own molecular and expansion microscopy expertise to the mix!
This is going to be the most exciting journey of my scientific career — more soon! 🌈🧠
The LMB is home to a unique community of outstanding connectomics scientists — I'm so excited about adding my own molecular and expansion microscopy expertise to the mix!
This is going to be the most exciting journey of my scientific career — more soon! 🌈🧠
Heart rate data or RC voltage step?
August 8, 2025 at 3:14 PM
Heart rate data or RC voltage step?
Saw someone designing a cloning strategy using chatgpt and for some reason that's kinda my breaking point in this particular discussion. How much thinking really needs to be outsourced for the sake of efficiency and who does that really serve in the end?
July 17, 2025 at 4:18 PM
Saw someone designing a cloning strategy using chatgpt and for some reason that's kinda my breaking point in this particular discussion. How much thinking really needs to be outsourced for the sake of efficiency and who does that really serve in the end?
Reposted by Richard Held
I have lots of opinions—but for your sake, I’ve limited them to segmentation in cryo-ET for this article 😉
www.sciencedirect.com/science/arti...
#teamtomo
www.sciencedirect.com/science/arti...
#teamtomo
Segmenting cryo-electron tomography data: Extracting models from cellular landscapes
Cryo-electron tomography provides an unprecedented view of cellular architecture, yet extracting meaningful biological insights remains challenging. S…
www.sciencedirect.com
July 11, 2025 at 12:46 AM
I have lots of opinions—but for your sake, I’ve limited them to segmentation in cryo-ET for this article 😉
www.sciencedirect.com/science/arti...
#teamtomo
www.sciencedirect.com/science/arti...
#teamtomo
Reposted by Richard Held
"The money the government sends to Harvard is, in effect, not a subsidy to advance the university’s mission. It’s a payment for the role Harvard plays in advancing the research mission of the United States." www.nytimes.com/interactive/...
Here Is All the Science at Risk in Trump’s Clash With Harvard (Gift Article)
More than 900 research grants worth $2.6 billion are in jeopardy. So is the 80-year-old model of American science.
www.nytimes.com
June 23, 2025 at 6:48 PM
"The money the government sends to Harvard is, in effect, not a subsidy to advance the university’s mission. It’s a payment for the role Harvard plays in advancing the research mission of the United States." www.nytimes.com/interactive/...
Boston is the best city to run in. This is a take I'm willing to fight over.
June 21, 2025 at 4:07 PM
Boston is the best city to run in. This is a take I'm willing to fight over.
Having come up through US academia the rug pull of the last few months has been pretty extreme. Wondering how much longer I (& everyone else) get to do science sucks but with some dead-man's logic it also makes me really appreciate the years that I got to spend asking questions for their own sake.
June 20, 2025 at 11:37 PM
Having come up through US academia the rug pull of the last few months has been pretty extreme. Wondering how much longer I (& everyone else) get to do science sucks but with some dead-man's logic it also makes me really appreciate the years that I got to spend asking questions for their own sake.