Filippo Caschera
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stjle.bsky.social
Filippo Caschera
@stjle.bsky.social
130 followers 220 following 90 posts
Synthetic Biology, Artificial Cells, Amphiphile Systems, Machine Learning, Cell-Free Protein Synthesis: Italy, Denmark, Japan, USA, France, Germany, UK.

Cell-Free Protein Synthesis consultant

https://scholar.google.com/citations?user=u6OhA2oAAAAJ&hl=en
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Filippo Caschera @stjle.bsky.social · Feb 19
Pleased to share my latest review on Cell-Free Protein Synthesis #CFPS and #AI/ML for accelerating drug discovery

www.sciencedirect.com/science/arti...

#CellFree #SynBio
Cell-free protein synthesis platforms for accelerating drug discovery
Cell-free protein synthesis is a platform for streamlined production of macromolecules. Recently, several proteins with pharmaceutical relevance were …
www.sciencedirect.com
stjle.bsky.social
High-throughput methods leveraging robotics and computer vision for the development of therapeutic phage cocktails

www.nature.com/articles/s41...
High-throughput methods leveraging robotics and computer vision for the development of therapeutic phage cocktails - Nature Communications
In this work, authors present an automated, high-throughput platform that utilizes robotics and computer vision to enable creation of a therapeutic phage cocktail, LBP-EC01, effective against over 96%...
www.nature.com
February 15, 2026 at 3:16 AM
stjle.bsky.social
Efficient Preparation of Homogenous Antibody Conjugates via Glycosite‐Specific Transglycosylation Enabled by Readily Available Glycosyl Donors - onlinelibrary.wiley.com/doi/full/10....
Efficient Preparation of Homogenous Antibody Conjugates via Glycosite‐Specific Transglycosylation Enabled by Readily Available Glycosyl Donors
A streamlined platform for glycosite-specific antibody conjugation is enabled by LacNAc-derived cyclic acetal donors, which can be prepared in only two steps and used directly in single-enzyme Endo S....
onlinelibrary.wiley.com
February 14, 2026 at 1:26 PM
stjle.bsky.social
www.nature.com/articles/s41...
A synthetic cell with integrated DNA self-replication and lipid biosynthesis - Nature Communications
The emergence and persistence of cellular life are the result of the integration of metabolic and genetic networks. Here the authors create a model synthetic cell that produces the key building blocks...
www.nature.com
February 13, 2026 at 5:44 PM
stjle.bsky.social
doi.org/10.7554/eLif...
Receptor Proteins: Lipids challenge ligands to control receptors
The behaviour of a receptor protein can be influenced by the presence of certain lipids in the membrane it is embedded in.
doi.org
February 13, 2026 at 5:18 AM
stjle.bsky.social
www.science.org/doi/10.1126/...
Structural ontogeny of protein-protein interactions
Understanding how protein binding sites evolve interactions with other proteins could hold clues to targeting “undruggable” surfaces. We used synthetic coevolution to engineer new interactions between...
www.science.org
February 13, 2026 at 3:58 AM
Reposted by Filippo Caschera
biorxiv-synthbio.bsky.social
Artificial Recombination of Nonribosomal Peptide Synthetases Rapidly Evolves Natural Products https://www.biorxiv.org/content/10.64898/2026.02.09.704528v1
February 11, 2026 at 11:04 PM
stjle.bsky.social
Integrated microdroplet workflow for high-throughput cell-free transcription in double emulsion picoreactors www.biorxiv.org/content/10.1...
www.biorxiv.org
February 11, 2026 at 4:44 AM
stjle.bsky.social
Slowpoke: An Automated Golden Gate Cloning Workflow for Opentrons OT-2 and Flex | ACS Synthetic Biology pubs.acs.org/doi/10.1021/...
Slowpoke: An Automated Golden Gate Cloning Workflow for Opentrons OT-2 and Flex
In synthetic biology, DNA assembly is a routine process where increasing demands for standardization, high-throughput capacity, and error-free execution are driving the development of accessible, auto...
pubs.acs.org
February 10, 2026 at 5:32 AM
stjle.bsky.social
Prototyping Minimal Extracellular Vesicle Mimetics Using Cell-Free Synthesis | ACS Nano pubs.acs.org/doi/10.1021/...
Prototyping Minimal Extracellular Vesicle Mimetics Using Cell-Free Synthesis
The many surface proteins on extracellular vesicles (EVs) allow them to target recipient cells and modulate cellular responses. Despite their importance, relating surface protein and EV function is ch...
pubs.acs.org
February 6, 2026 at 5:08 PM
stjle.bsky.social
www.nature.com/articles/s44...
The status of extracellular vesicles as drug carriers and therapeutics - Nature Reviews Bioengineering
Extracellular vesicles (EVs) are emerging as biocompatible carriers for targeted drug delivery, yet challenges in yield, cargo loading and biodistribution persist. In this Review, key trends from over...
www.nature.com
February 6, 2026 at 11:23 AM
stjle.bsky.social
An AI-Native Biofoundry for Autonomous Enzyme Engineering: Integrating Active Learning with Automated Experimentation

www.biorxiv.org/content/10.6...
www.biorxiv.org
February 2, 2026 at 2:02 PM
Reposted by Filippo Caschera
biorxiv-synthbio.bsky.social
Topological reprogramming transforms an integral membrane oligosaccharyltransferase into a water-soluble glycosylation catalyst https://www.biorxiv.org/content/10.64898/2026.01.30.702934v1
February 1, 2026 at 10:02 AM
stjle.bsky.social
journals.plos.org/plosbiology/...
Microgravity reshapes bacteriophage–host coevolution aboard the International Space Station
Phage-host interactions have a role shaping microbial ecosystems, but their behavior in microgravity remains largely unexplored. In this study, experiments aboard the ISS reveal distinct evolutionary ...
journals.plos.org
January 25, 2026 at 3:13 AM
stjle.bsky.social
www.nature.com/articles/s41...
An engineered UGA suppressor tRNA gene for disease-agnostic AAV delivery - Nature Biotechnology
Therapeutic suppressor tRNAs are engineered to broaden their target range in vivo.
www.nature.com
January 20, 2026 at 12:27 PM
stjle.bsky.social
www.biorxiv.org/content/10.6...
A continuous viral vaccine biomanufacturing platform utilizing multiple bioreactor configurations
Scalable, continuous biomanufacturing processes have grown in importance to meet demand for smaller bioreactor sizes, lowered production costs, and improved quality attributes. The Sf9/recombinant bac...
www.biorxiv.org
January 12, 2026 at 2:08 PM
stjle.bsky.social
analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/...
A Guide to Bayesian Optimization in Bioprocess Engineering
Bayesian optimization has become widely popular across various experimental sciences due to its favorable attributes: it can handle noisy data, perform well with relatively small data sets, and provi...
analyticalsciencejournals.onlinelibrary.wiley.com
January 10, 2026 at 10:06 PM
stjle.bsky.social
Molecular systems engineering of synthetic cells

www.nature.com/articles/s41...
January 10, 2026 at 9:12 AM
stjle.bsky.social
www.biorxiv.org/content/10.6...
Enhanced tRNA array method version 2 for simultaneous in vitro synthesis of 21 tRNAs
Transfer RNAs (tRNAs) play an essential role in translation, and their simultaneous in vitro synthesis remains a key challenge in bottom-up synthetic biology. We previously developed the tRNA array me...
www.biorxiv.org
January 8, 2026 at 8:55 PM
Reposted by Filippo Caschera
chofski.bsky.social
If you are looking to integrate your latest genetic creation into the genome of your favourite bug, you might find our latest review now out in OUP Synthetic Biology useful. 🧬⚒️ Work led by Riesa Rohmat with input from Thea Irvine and Shivang Joshi. #genome #synbio doi.org/10.1093/synb...
January 6, 2026 at 10:58 AM
stjle.bsky.social
www.biorxiv.org/content/10.1...
Harnessing protein-folding algorithms to drug intrinsically disordered epitopes
Due to their lack of a specific structure and dynamical nature, targeting of epitopes that are part of an intrinsically disordered region of a protein is a notoriously difficult task. Here, we describ...
www.biorxiv.org
January 5, 2026 at 7:15 AM
stjle.bsky.social
pubs.acs.org/doi/10.1021/...
The Role of Intrinsically Disordered Domains in Regulating G Protein-Coupled Receptor Signaling
The α2A adrenergic receptor (α2AAR) is a clinically important target for various diseases including hypertension, diabetes, and chronic pain. Here, using single-molecule fluorescence resonance energy ...
pubs.acs.org
January 1, 2026 at 11:23 AM
Reposted by Filippo Caschera
biorxiv-synthbio.bsky.social
Planning and scheduling biological experiments across multiple liquid handling robots https://www.biorxiv.org/content/10.64898/2025.12.26.696584v1
December 29, 2025 at 7:02 PM
stjle.bsky.social
academic.oup.com/nar/article/...
Engineered chimeric T7 RNA polymerase improves salt tolerance and reduces dsRNA impurity generation during in vitro transcription of mRNA
Abstract. Messenger RNA (mRNA) is typically produced enzymatically through in vitro transcription using wild-type T7 RNA polymerase; however, this enzyme i
academic.oup.com
December 29, 2025 at 7:00 PM
stjle.bsky.social
pubs.acs.org/doi/10.1021/...
Recoded Bacteriophage Genome for Bio-Orthogonal-Enabled Concentration and Detection of E. coli in Drinking Water
Modern genome editing methods permit the flexible modification of organisms at the genome level. However, bacteriophages, despite their small genomes, pose unique challenges due to the need to edit du...
pubs.acs.org
December 29, 2025 at 1:22 PM
stjle.bsky.social
A Simple and Versatile Cell-Free Expression Method for Producing Secondary Metabolites | ACS Synthetic Biology pubs.acs.org/doi/10.1021/...
A Simple and Versatile Cell-Free Expression Method for Producing Secondary Metabolites
Secondary metabolites are a major source of natural products with industrially relevant bioactivities. Lysate-based cell-free expression (CFE) is an emerging platform for accelerating the discovery an...
pubs.acs.org
December 27, 2025 at 7:58 AM