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Abhi Aggarwal

@abhiaggarwal.bsky.social

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Abhi Aggarwal @abhiaggarwal.bsky.social · Mar 25

🚨iGluSnFR4 is finally out!🚨🧪

We present iGluSnFR4f and 4s, a novel pair of genetically-encoded glutamate indicators designed for high-fidelity imaging of synaptic activity in the living brain. ⬇️

www.biorxiv.org/content/10.1...

🎥 Below: iGluSnFR4s detecting minis in cultures w/ TTX

#Neuroscience

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Abhi Aggarwal @abhiaggarwal.bsky.social · Jul 31

We haven’t observed any clear signs of cytotoxicity or aggregation, in contrast to what we’ve occasionally seen with jRGECO1a. We imaged at around 2 weeks in cultured cells and 4–6 weeks in vivo.

Abhi Aggarwal @abhiaggarwal.bsky.social · Jul 31

#OCaMP is now on bioRxiv and reagents are available on #Addgene! 🧪

A sensitive orange fluorescent calcium ion indicator for imaging neural activity

www.biorxiv.org/content/10.1...

A sensitive orange fluorescent calcium ion indicator for imaging neural activity

Genetically encoded calcium indicators (GECIs) are vital tools for fluorescence-based visualization of neuronal activity with high spatial and temporal resolution. However, current highest-performance...

www.biorxiv.org

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Abhi Aggarwal @abhiaggarwal.bsky.social · Mar 25

Where can I get reagents?

Bacterial (pRSET) and mammalian (pAAV) expression vectors, including different promoters, Cre- and Flp- dependent vectors, and both the NGR and PDGFR membrane targeting sequences, are available from @addgene.bsky.social. /3

www.addgene.org/browse/artic...

Addgene: iGluSnFR4 Plasmids 2025

www.addgene.org

Abhi Aggarwal @abhiaggarwal.bsky.social · Mar 25

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Abhi Aggarwal @abhiaggarwal.bsky.social · Mar 25

Hi Dani! Can you add me whenever you get a chance?
scholar.google.com/citations?us...

Abhi Aggarwal @abhiaggarwal.bsky.social · Mar 25

Thanks for organizing, can you add me?
scholar.google.com/citations?us...

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Abhi Aggarwal @abhiaggarwal.bsky.social · Mar 25

Hi, thanks for organizing. Can you add me?
scholar.google.com/citations?us...

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Reposted by Abhi Aggarwal

Wonder of Science @wonderofscience.bsky.social · Feb 1

High-speed video showing glutamate lighting up as it’s released in synapses, visualizing the neurotransmitters of the brain with the fluorescent indicator protein iGluSnFR3.

Credit: Allen Institute, Aggarwal et al.

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Abhi Aggarwal @abhiaggarwal.bsky.social · Nov 12

We’re hoping to have the preprint out within the next month or two🤞

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Abhi Aggarwal @abhiaggarwal.bsky.social · Nov 12

First sky post 🦋 Sharing some vibrant iGluSnFR3 minis in cultured neurons!

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Abhi Aggarwal @abhiaggarwal.bsky.social · Nov 12

I’d love to be added! 😊

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