Alex Marzilli
@alex-marzilli.bsky.social
Reposted by Alex Marzilli
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
September 22, 2025 at 6:27 PM
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Reposted by Alex Marzilli
Messages loud & clear: Chris & Alex designed destabilized MS2 and PP7 coat proteins, which are degraded in cells except when bound to their cognate RNA hairpins. We used these probes to image distinct single mRNAs simultaneously in live cells, 'background-free.' AddGene soon! tinyurl.com/27p7hyt9
November 22, 2024 at 3:39 AM
Messages loud & clear: Chris & Alex designed destabilized MS2 and PP7 coat proteins, which are degraded in cells except when bound to their cognate RNA hairpins. We used these probes to image distinct single mRNAs simultaneously in live cells, 'background-free.' AddGene soon! tinyurl.com/27p7hyt9