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alexlouiseshaw.bsky.social
Alexandria Shaw
@alexlouiseshaw.bsky.social
310 followers 290 following 4 posts
PhD Candidate at UBC Biochemistry with John Burke and Calvin Yip | CIHR Doctoral Scholar | Structural Biology | Cryo-EM | HDX-MS | Kinases and Phospholipids | 🇨🇦 | Seeking a postdoctoral position
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Reposted by Alexandria Shaw
hrmem.bsky.social
HRMEM @hrmem.bsky.social · May 14
HRMEM is happy to spotlight this structure of calcineurin bound to PI4KA determined by Alexandria Shaw and colleagues from the Burke Lab at the University of Victoria and the Yip Lab at UBC!

We are proud to have provided the electron microscopy for this work!

Read more:
doi.org/10.1016/j.st...
Structure of calcineurin bound to PI4KA reveals dual interface in both PI4KA and FAM126A
Phosphatidylinositol 4-kinase alpha (PI4KA) maintains the phosphatidylinositol 4-phosphate (PI4P) and phosphatidylserine pools of the plasma membrane.…
www.sciencedirect.com
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Reposted by Alexandria Shaw
jburkevic.bsky.social
John Burke @jburkevic.bsky.social · 28d
New review out from my lab on class I PI3Ks www.sciencedirect.com/science/arti..., was fun to write where we are and where we are going in PI3K research
Great work from Isobel and Alex in my lab @alexlouiseshaw.bsky.social
Molecular basis for regulation of the class I phosphoinositide 3-kinases (PI3Ks), and their targeting in human disease
The class I phosphoinositide 3-kinase pathway (PI3K) is a master regulator of cellular growth, and plays essential roles in controlling immune cell fu…
www.sciencedirect.com
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Reposted by Alexandria Shaw
redpenblackpen.bsky.social
RedPen/BlackPen @redpenblackpen.bsky.social · Aug 8
Really just had something like this feeling this afternoon. It’s the high that keeps you coming back for more
Cartoon of a scientist peering through a microscope saying "Whoaah!!!". Title says "The best part of Science is knowing, for a moment, something that nobody else in the world knows."
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Reposted by Alexandria Shaw
sushantsuresh.bsky.social
Sushant Suresh @sushantsuresh.bsky.social · Aug 7
Great collaborative work where we developed a nanobody based tool to block EFR3 mediated recruitment of PI4KA. A potential therapeutic window maybe?
Thanks @alexlouiseshaw.bsky.social for the assist!
alexlouiseshaw.bsky.social
Alexandria Shaw @alexlouiseshaw.bsky.social · Aug 6
New preprint from the lab headed up by
@sushantsuresh.bsky.social! Late last year, we published the molecular basis of PI4KA plasma membrane recruitment by EFR3 (PMID:39705356). This work builds on these findings and identifies a new potential therapeutic avenue! Check it out!
biorxivpreprint.bsky.social
bioRxivpreprint @biorxivpreprint.bsky.social · Aug 1
Development of an inhibitory TTC7B selective nanobody that blocks EFR3 recruitment of PI4KA https://www.biorxiv.org/content/10.1101/2025.07.28.667261v1
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alexlouiseshaw.bsky.social
Alexandria Shaw @alexlouiseshaw.bsky.social · Aug 6
New preprint from the lab headed up by
@sushantsuresh.bsky.social! Late last year, we published the molecular basis of PI4KA plasma membrane recruitment by EFR3 (PMID:39705356). This work builds on these findings and identifies a new potential therapeutic avenue! Check it out!
biorxivpreprint.bsky.social
bioRxivpreprint @biorxivpreprint.bsky.social · Aug 1
Development of an inhibitory TTC7B selective nanobody that blocks EFR3 recruitment of PI4KA https://www.biorxiv.org/content/10.1101/2025.07.28.667261v1
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alexlouiseshaw.bsky.social
Alexandria Shaw @alexlouiseshaw.bsky.social · Jul 7
New review out with @jburkevic.bsky.social!
We highlight recent advances in our understanding of the role the phosphoinositide PIP3 plays in regulating the protein kinases Akt, PDK1, and BTK from a structural perspective.
#kinase #phosphoinositides

portlandpress.com/biochemsoctr...
Molecular insight on the role of the phosphoinositide PIP3 in regulating the protein kinases Akt, PDK1, and BTK
Protein kinases are master regulators of myriad processes in eukaryotic cells playing critical roles in growth, metabolism, cellular differentiation, and motility. A subclass of protein kinases is reg...
portlandpress.com
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Reposted by Alexandria Shaw
joshdcryoem.bsky.social
Josh Dickerson @joshdcryoem.bsky.social · Apr 23
After years parked at a Polara, I’m excited to share that my PhD work is finally out! We figured out why imaging at liquid helium temperatures wasn’t working—and how to fix it.
🔗 www.pnas.org/doi/10.1073/...
Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures | PNAS
The physical limit in determining the atomic structure of biological molecules is radiation damage. In electron cryomicroscopy, there have been num...
www.pnas.org
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Reposted by Alexandria Shaw
jburkevic.bsky.social
John Burke @jburkevic.bsky.social · Mar 31
New work out from my lab by my graduate student Isobel in collaboration with Josh Pemberton and Tamas Balla. Provides some new cool tools to manipulate PI3,5P2 levels, as well as some new insight into PIKfyve regulation.
rupress.org/jcb/article-...
An advanced toolset to manipulate and monitor subcellular phosphatidylinositol 3,5-bisphosphate
In this paper, Pemberton et al. describe the generation and characterization of an engineered active fragment of the PIKfyve enzyme and its use to generate
rupress.org
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Reposted by Alexandria Shaw
jcb.org
Journal of Cell Biology @jcb.org · Apr 2
An advanced toolset to manipulate and monitor subcellular phosphatidylinositol 3,5-bisphosphate. New study from Joshua Pemberton, John Burke, Tamas Balla (National Institute of Child Health and Human Development) and colleagues: rupress.org/jcb/article/...

#Biochemistry #CellSignaling #lipid
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Reposted by Alexandria Shaw
sushantsuresh.bsky.social
Sushant Suresh @sushantsuresh.bsky.social · Jan 6
Back from vacation and really glad to share some work from my PhD research. Great collaborative effort @alexlouiseshaw.bsky.social Check out this post from my PI to learn more about Lipid kinase PI4KA and its regulation!
jburkevic.bsky.social
John Burke @jburkevic.bsky.social · Dec 20
First post up, sharing some cool work from my lab by @alexlouiseshaw.bsky.social and @sushantsuresh.bsky.social out today in Science Advances (www.science.org/doi/10.1126/...)
Molecular basis for plasma membrane recruitment of PI4KA by EFR3
The structure of the EFR3A-PI4KA complex reveals insight into PI4KA membrane localization and its role in human disease.
www.science.org
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alexlouiseshaw.bsky.social
Alexandria Shaw @alexlouiseshaw.bsky.social · Dec 21
No better way to start the holidays then being able to share that some of my PhD research is live on Science Advances today!😄 A great collaborative effort! Check out this thread from my PI!
jburkevic.bsky.social
John Burke @jburkevic.bsky.social · Dec 20
First post up, sharing some cool work from my lab by @alexlouiseshaw.bsky.social and @sushantsuresh.bsky.social out today in Science Advances (www.science.org/doi/10.1126/...)
Molecular basis for plasma membrane recruitment of PI4KA by EFR3
The structure of the EFR3A-PI4KA complex reveals insight into PI4KA membrane localization and its role in human disease.
www.science.org
alexlouiseshaw.bsky.social
Alexandria Shaw @alexlouiseshaw.bsky.social · Dec 11
Could I be added to this please? Thanks! 😀
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