We thank this our institutions for supporting this 🇦🇷🇦🇺 collaboration! Thanks @unsamoficial.bsky.social @muche-macquarie.bsky.social
And the many PhD Students and Posdocs implicated!

Our work establishes SCRaMbLE as a powerful bacterial genome engineering platform, opening new paths to study genome organization, evolution, and strain optimization.

Unexpectedly, some rearranged strains grew faster than the parental strain! These results reveal a striking phenotypic robustness to genome organization changes and support the idea that genome rearrangements can drive phenogenetic drift in bacteria.

After inducing rearrangements and selecting for fast growth, we found dramatically reconfigured genomes with stable physiology despite altered gene order. This technique enabled on‑demand, large‑scale genome rearrangements—inversions, duplications, and translocations across megabases.

We placed 14 loxPsym sites to enable inversions, duplications, and translocations while preserving near‑wild‑type fitness before rearrangement. Including essential genes allowed avoiding deletions.

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