Very happy to be at #microevo25 in Liverpool! Here’s to two days of micro-awesomeness and Beatles’ references!

In fact, deleting blaOXA-48 from pOXA-48 strongly decreases the cost associated with the plasmid. 10/14

Lastly, we show that the main contributor to pOXA-48 fitness cost is the gene encoding for the β-lactamase, blaOXA-48. The individual cost of blaOXA-48 strongly correlates with the overall cost of pOXA-48, specially in Klebsiella pneumoniae. 9/14

pOXA-48 is more stable in Klebsiella pneumoniae than in Escherichia coli, which we hypothesise can be either due to differences in the efficiency of the partition machinery or in the copy number of the plasmid between the two species. 8/14

We identify the genes important for pOXA-48 across the different strains. Some of them are involved in plasmid maintenance and mitigation of plasmid-cost by modulation of gene expression. Individually silencing of these genes leads to plasmid-loss at different rates in the several strains. 6/14

The genes encoded in the plasmid produce similar fitness effects across the different enterobacterial hosts. There are small differences between strains, but we observe a clear general trend across them. 5/14

We know that pOXA-48 leads to very different fitness effects in its several bacterial hosts. The question is, what are the genetic basis behind said differences? We perform CRISPRi screens in different clinical bacteria to study the role of each plasmid gene in fitness cost and plasmid biology. 3/14

Today, we Portuguese around the globe celebrate #25deAbril, also known as the Carnation Revolution. As fights for Democracy go, this one is a particularly beautiful one. 25 de Abril sempre, fascismo nunca mais 🌹

Happy International Day of Women and Girls in Science 👩‍🔬🧬 #womeninscience #girlsinscience

In fact, deleting blaOXA-48 from pOXA-48 strongly decreases the cost associated with the plasmid. 10/14

Lastly, we show that the main contributor to pOXA-48 fitness cost is the gene encoding for the β-lactamase, blaOXA-48. The individual cost of blaOXA-48 strongly correlates with the overall cost of pOXA-48, specially in Klebsiella pneumoniae. 9/14

pOXA-48 is more stable in Klebsiella pneumoniae than in Escherichia coli, which we hypothesise can be either due to differences in the efficiency of the partition machinery or in the copy number of the plasmid between the two species. 8/14

We identify the genes important for pOXA-48 across the different strains. Some of them are involved in plasmid maintenance and mitigation of plasmid-cost by modulation of gene expression. Individually silencing of these genes leads to plasmid-loss at different rates in the several strains. 6/14

The genes encoded in the plasmid produce similar fitness effects across the different enterobacterial hosts. There are small differences between strains, but we observe a clear general trend across them. 5/14

We know that pOXA-48 leads to very different fitness effects in its several bacterial hosts. The question is, what are the genetic basis behind said differences? We perform CRISPRi screens in different clinical bacteria to study the role of each plasmid gene in fitness cost and plasmid biology. 3/14