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Reposted by Ana Burgos

Zebrafish Rock! @zebrafishrock.bsky.social · Apr 9

Ageing

#TenaLab @cabd-upo-csic.bsky.social ‪@anaburgos.bsky.social‬ (Killifish/ scRNAseq/ Aging)
https://www.biorxiv.org/content/10.1101/2025.03.24.645095

Special Topics

@aburger2009.bsky.social (Data Reporting/ Metadata/ AI)
https://osf.io/preprints/osf/kvn2x (27/34)

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Reposted by Ana Burgos

Zebrafish Rock! @zebrafishrock.bsky.social · Apr 9

@obog.bsky.social @anaburgos.bsky.social‬ (Spermatogenesis/ Multiomics/ Epigenetics)
https://www.biorxiv.org/content/10.1101/2025.03.12.642371 (21/34)

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Reposted by Ana Burgos

bioRxivpreprint @biorxivpreprint.bsky.social · Mar 25

Neotenic transcriptomic features in the adult turquoise killifish brain https://www.biorxiv.org/content/10.1101/2025.03.24.645095v1

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Ana Burgos @anaburgos.bsky.social · Mar 25

For even more in-depth analyses, check out our preprint! A huge thank you to all who worked on this project— @gndc.bsky.social @jlopezrios.bsky.social #silvianaranjo #vallehernandez #juanjtena #martamoreno #anafernandezmiñan #lorenaardila #alejandrodelosreyes

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Ana Burgos @anaburgos.bsky.social · Mar 25

While killifish is a powerful model for studying aging, extrapolating results to mammals should be done cautiously. Nonetheless, its neotenic brain traits might help identify promising targets for regenerative or anti-aging therapies. 8/9

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Ana Burgos @anaburgos.bsky.social · Mar 25

In vertebrates, embryos typically show higher cell proliferation rates compared to adults. Our results suggest that the neotenic traits we observed in the adult killifish brain could support rapid growth and/or help mitigate challenges associated with its high metabolic rate. 7/9

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Ana Burgos @anaburgos.bsky.social · Mar 25

To test if embryonic gene expression in adult killifish brains is incidental or a broader trend, we compared our data to zebrafish single-cell profiles from adult brains and embryos. Remarkably, killifish brains aligned more with embryonic profiles. 6/9

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Ana Burgos @anaburgos.bsky.social · Mar 25

Our findings are further supported by the identification of primitive hematopoietic progenitor cells (HPC), present in all 7 single-cell adult samples, along with primitive erythrocytes expressing embryonic hemoglobin genes. 5/9

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Ana Burgos @anaburgos.bsky.social · Mar 25

Surprisingly, we found a fascinating feature: evidence of primitive hematopoiesis in the adult killifish brain. We identified stromal cell clusters (EC & Mural runx1⁺) with mixed hemangioblast/hemogenic traits—similar to rapid transitions observed in embryonic zebrafish. 4/9

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Ana Burgos @anaburgos.bsky.social · Mar 25

We used single-cell RNA-seq to profile brain samples from killifish across their entire adult lifespan—from young adulthood to old age—in both males and females, with the goal of understanding how aging unfolds in the brain. 3/9

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Ana Burgos @anaburgos.bsky.social · Mar 25

Killifish is one of the shortest-lived vertebrates on Earth. It inhabits seasonal ponds and has evolved to grow, reproduce, and age within just a few months. Due to its rapid life cycle, it is now widely used as a model to study aging and lifespan regulation in real time. 2/9

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Ana Burgos @anaburgos.bsky.social · Mar 25

Can adult brains retain embryonic traits? Our latest work reports neotenic transcriptomic features in the adult turquoise killifish brain—suggesting that some embryonic programs remain active throughout life. 1/9 🧠🐟👇
www.biorxiv.org/content/10.1...

www.biorxiv.org

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Ana Burgos @anaburgos.bsky.social · Mar 13

Finally, big thanks to everyone involved in this project, your contributions were essential in making this research possible: Fan-Suo G, Gala P, Thirsa B, Estefanía S, María Almuedo, Juan J Tena, @obog.bsky.social @aruizherrera.bsky.social @ageinves.bsky.social @cabd-upo-csic.bsky.social and others

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Ana Burgos @anaburgos.bsky.social · Mar 13

In elongated spermatids, despite chromatin compaction and transcriptional silencing, thousands of sites remain accessible which coincide with CpG islands and which might be retained intergenerationally to aid with early developmental processes in the next generation. 6/7

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Ana Burgos @anaburgos.bsky.social · Mar 13

What about chromatin? scATAC-seq show local and global remodeling. We identify thousands of locus-specific events and track chromatin accessibility rising during spermatogonial differentiation, peaking in spermatocytes, and dropping in spermatids, paralleling txn shutdown. 5/7

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Ana Burgos @anaburgos.bsky.social · Mar 13

Our WGBS data reveal a stable 5mCG landscape across spermatogenesis. However, we detected ~4000 localized changes in spermatocytes that coincide with CpG islands and that might reflect structural or gene-regulatory events associated with these cells. 4/7

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Ana Burgos @anaburgos.bsky.social · Mar 13

Next, we wanted to know which genes drive the transformation of spermatogonial cells into mature sperm. Using pseudotime analysis methods, we mapped their differentiation paths and identified ~160 key gene drivers, helping to decode the molecular blueprint of spermatogenesis. 3/7

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Ana Burgos @anaburgos.bsky.social · Mar 13

Using single-cell omics (scRNA-seq, scATAC-seq) and WGBS from sorted germ cell populations we mapped the entire journey! Our scRNA-seq data reveal diverse populations ranging from undifferentiated spermatogonia to elongated spermatids. 2/7

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Ana Burgos @anaburgos.bsky.social · Mar 13

Ever wondered how sperm are formed? Spermatogenesis is a microscopic marathon that starts in the testes and ends with fully functional sperm! Our new preprint bitly.cx/jC4Qg takes a deep dive into zebrafish spermatogenesis, mapping out every fascinating step. 🧵👇 1/7

A single-cell multiomics roadmap of zebrafish spermatogenesis reveals regulatory principles of male germline formation

Spermatogenesis is the biological process by which male sperm cells (spermatozoa) are produced in the testes. Beyond facilitating the transmission of genetic information, spermatogenesis also provides...

bitly.cx

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Ana Burgos @anaburgos.bsky.social · Jun 29

Hi! I am building joinportiko.com a free platform for researchers to find collaborators easily. We are now 500+ researchers open to collaborate using the techniques , analysis or experiments we have expertise in :)
It would be helpful if you repost this to create a bigger community :) thanks ! 💕

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Ana Burgos @anaburgos.bsky.social · Jun 20

Thanks ♥️

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Ana Burgos @anaburgos.bsky.social · Jun 20

thank you! ☺️ I hope this can help as much ppl as possible ✨

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Ana Burgos @anaburgos.bsky.social · Jun 20

🥹♥️ I would love to eliminate the bias when it comes to opportunities for collaboration.

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Ana Burgos @anaburgos.bsky.social · Jun 19

I launched a platform for researchers to find collaborations easily. A kind of blablacar or airbnb but instead of putting your route or apartment, you put the technique, experiment or analysis that you handle and with which you would be open to make collaborations. joinportiko.com
re-post please!

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