AbstractBackground and Aims. Platelets are essential for haemostasis, thrombosis, and inflammation, with their functions controlled by receptor signalling

Up to 1 billion cardiomyocytes (CMs) die during myocardial infarction (MI), leading to permanent muscle loss and devasting functional impacts. Biomate…

Background Collective migration is the coordinated movement of a group of cells—a fundamental process in health and disease. Many models have been developed to study the molecular and physical mechanisms of collective migration. However, the aim of this study is to engineer a flexible in vitro framework that allows for mechanobiological quantification of the separate and combined contributions of individual cell mechanics to the directed migration of a collective. We utilised this framework to understand the role of juxtacrine Notch signalling during collective endothelial migration—an essential process during the formation of new blood vessels (known as angiogenesis). Results This framework enables users to perform high spatiotemporal analysis of migrative behaviour, cell–matrix traction forces, and intercellular forces in different microenvironments. With this framework, we show that Notch inhibited collectives adopt a distinct regime of directed collective migration. Whereas the directionality of migration, traction forces and intercellular forces are not affected by Notch inhibition, we observed spatiotemporal differences in migration speed, traction force magnitude and normal and shear stresses within Notch-inhibited collectives. Conclusions The in vitro framework is a powerful approach for dissecting the mechanisms of collective migration. With this framework, we show that a potential link exists between the juxtacrine signalling of Notch and an increased mechanical cohesiveness among collective cells.

Abstract. Multicellular spheroids have shown great promise in 3D biology. Many techniques exist to form spheroids, but how cells take mechanical advantage

Bhawana Singh, Ioanna Tzoulaki, Manuel Mayr; Precision medicine requires precision proteomics: Discordance between proteomic and clinical assays in UK Biob

Cardiovascular disease, arteriosclerosis, is characterized by the thickening of blood vessel (arteries) walls restricting blood flow and is a global health prob...

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Limited vascularization and ischemia are major contributors to the chronicity of wounds, such as ulcers and traumatic injuries, which impose significant medical, social, and economic burdens. These ch...

The valine catabolite 3-hydroxyisobutyrate (3-HIB) is suggested to mediate the uptake of extracellular fatty acids into the cells, thus regulating int…

We present a case of a child presenting with recurrent myocarditis 5 years before developing dilated cardiomyopathy with a novel filamin C truncating variant. Complicated myocarditis in children is co...

IntroductionTissue Engineering (TE) uses resorbable polymers to promote in-situ cellular growth, transforming the implant into a living valve. This study cha...

ISACB is searching for candidates to serve as new members of our Committees and Executive Council. We welcome applications from members at all career stages who wish for the opportunity to contribute ...

Cardiomyopathy (CMP) is the leading cause of death in Duchenne muscular dystrophy (DMD). Characterization of disease trajectory can be challenging, es…

Medical devices such as vascular grafts, stents, and catheters are crucial for patient treatment but often suffer suboptimal integration with host tissues due to the nature of their surfaces. The mate...

Abstract. Introduction: Abdominal aortic aneurysm (AAA) is a multifactorial disease with limited identification of contributing genetic factors. p27kip, also known as CDKN1B, is a cell cycle inhibitor...

Mitochondrial ROS activate STAT5 to drive foam cell formation, inhibiting STAT5 alters macrophage metabolism and stress response.

An increasing number of individuals are at high risk of type 2 diabetes (T2DM) and its cardiovascular (CV) complications, which challenges healthcare systems with an increased risk of developing CV d...

The arteriovenous fistula (AVF) is the gold standard for hemodialysis vascular access, although inadequate vascular remodelling and intimal hyperplasia pose a major limitation. It is essential to stud...

Controlling the formation of new blood vessels, i.e. angiogenesis, is a critical challenge for the success of regenerative medicine. The development of effective strategies is hindered by our incomple...