Lightnews — Scholar-powered news

Ask Zebrafish? @askzebrafish.bsky.social · 6d

🤔 #AskZebrafish: Would anyone within the EU have a mutant ybx1 line swimming around and willing to share? Any leads would be appreciated.

1 2

Ask Zebrafish? @askzebrafish.bsky.social · 6d

🤔 #AskZebrafish: Looking for krt18a reporter fish in the UK or if anyone has a ready to inject construct for making one and are willing to share, that would be much appreciated!

1 1 2

Ask Zebrafish? @askzebrafish.bsky.social · 6d

🤔 #AskZebrafish: Looking for Prom1a and/or Prom1b mutant lines and/or reporter lines that someone would be willing to share? Preferably in the USA.

Ask Zebrafish? @askzebrafish.bsky.social · Jun 20

🤔 Query from Slack: Looking for 5' myl7/cmlc2 promoter vector and someone willing to share it? Preferably in the US 🐟🫀 #AskZebrafish

Ask Zebrafish? @askzebrafish.bsky.social · Jun 9

Another query from Slack: Looking for ccdc40 mutant #zebrafish (aka locke). Preferably in the US. Any leads would be much appreciated! #AskZebrafish

1 1

Ask Zebrafish? @askzebrafish.bsky.social · Jun 9

Query from Slack: Does anyone have good recommendations for antibodies that label mitochondrial DNA encoded proteins and work well in #zebrafish? #AskZebrafish

1 1

Ask Zebrafish? @askzebrafish.bsky.social · May 15

Anonymous query from Slack: Does anyone have the TgBAC(foxp3a:TagCFP-NTR)vcc5 line swimming around, preferably in the US? Thanks in advance! #AskZebrafish 🤔

Ask Zebrafish? @askzebrafish.bsky.social · May 13

Still on the hunt for fish lines 🙏

Zebrafish Rock! @zebrafishrock.bsky.social · May 9

🤔 #AskZebrafish: Looking for a transgenic line that labels cranial muscles, preferably in the USA? #zebrafish

1

Ask Zebrafish? @askzebrafish.bsky.social · May 1

Still searching for leads! 🙏

An image of a spotlight with a zebrafish during a dark, rainy night. Akin to the bat signal.

1

Reposted by Ask Zebrafish?

Zebrafish Rock! @zebrafishrock.bsky.social · Apr 29

Head-scratcher from Slack: Does anyone know if it is possible to tell (by eye/microscope) if the developing oocytes in a zebrafish ovary are at the pre-, during, or post-vitellogenesis stage? 🤔 #AskZebrafish

2 4 2

Reposted by Ask Zebrafish?

Zebrafish Rock! @zebrafishrock.bsky.social · Apr 26

Looking for anyone w/ experience in smRNA analysis who can provide someone with information or guidance? They already have their sequences, but can't find a clear methodology/protocol to help them analyze it. The sequences were obtained from NOVOGENE's Small RNA Sequencing service. 🤔 #AskZebrafish

3 1 3

Ask Zebrafish? @askzebrafish.bsky.social · Apr 22

Any leads would be appreciated 🙏

1 2

Ask Zebrafish? @askzebrafish.bsky.social · Apr 8

Any leads would be appreciated! Reposts welcome 🙏

2 2

Ask Zebrafish? @askzebrafish.bsky.social · Apr 7

Still searching for leads 👀

2 1

Ask Zebrafish? @askzebrafish.bsky.social · Apr 4

Still looking for leads 🕵️

Reposted by Ask Zebrafish?

Zebrafish Rock! @zebrafishrock.bsky.social · Apr 4

Query from our Slack Tankspace: Does anyone in Europe have the Tg(mpx: mcherry ) line from the Huttenlocher Lab or similar and would be willing to share? #AskZebrafish

1 2 1

Ask Zebrafish? @askzebrafish.bsky.social · Apr 4

Still looking for leads 🕵️

1

Ask Zebrafish? @askzebrafish.bsky.social · Mar 31

Re-upping

Zebrafish Rock! @zebrafishrock.bsky.social · Mar 18

Query from Slack: Does anyone have scn8aa and scn8ab #zebrafish mutants and would be willing to share some embryos? (Preferably in Europe, if possible) 🔎 #AskZebrafish

Ask Zebrafish? @askzebrafish.bsky.social · Mar 31

Ping @bazzinilab.bsky.social Didn’t your team do something like this?

1

Ask Zebrafish? @askzebrafish.bsky.social · Mar 31

Did you find this yet?

Reposted by Ask Zebrafish?

Zebrafish Rock! @zebrafishrock.bsky.social · Mar 27

Another query from Slack: Does anyone (preferably in the US) have the Tg(GFAP:iGluSnFR) line and be willing to send them to another #zebrafish lab? 🤔 #AskZebrafish

1 2 3

Ask Zebrafish? @askzebrafish.bsky.social · Mar 27

🤔 #AskZebrafish

Zebrafish Rock! @zebrafishrock.bsky.social · Mar 27

Query from Slack: Does anyone have a plasmid construct with a dominant negative form of protein kinase A (PKA) or experience using it with zebrafish embryos? Any help and/or leads would be appreciated!

Ask Zebrafish? @askzebrafish.bsky.social · Mar 21

Thanks but we don’t think this would be classified as ‘efficiently’

Ask Zebrafish? @askzebrafish.bsky.social · Mar 21

Still looking for guide sequences! Any leads and/or help appreciated!

Zebrafish Rock! @zebrafishrock.bsky.social · Mar 19

Query for the greater scientific community: “Does anyone know of a guide RNA that efficiently targets mCherry?” #AskZebrafish 🤔

1

Reposted by Ask Zebrafish?

Zebrafish Rock! @zebrafishrock.bsky.social · Mar 19

🤔 #AskZebrafish

Stephanie Caty, PhD @stephcaty.bsky.social · Mar 19

I'm currently on the hunt to find a reference chemical that reduces neutrophil and/or macrophage counts that I can use as a positive control in this assay moving forward. Any suggestions are very welcome!

2 1