Ben Longdon
@blongdon.bsky.social
Virus evolution, ecology and host shifts. Fly zookeeper. Insect rhabdovirus geek. Tea Junkie. Like music, food, bikes. Lab website: www.benlongdon.com
Fresh buffer won't have the residual EtBr mixed in with it, so can sometimes cause a front (where EtBr migrates out of gel) that can look something like this - but this seems quite extreme! Mix a little EtBr into negative end of gel tank and see if it resolves?
February 5, 2026 at 3:28 PM
Fresh buffer won't have the residual EtBr mixed in with it, so can sometimes cause a front (where EtBr migrates out of gel) that can look something like this - but this seems quite extreme! Mix a little EtBr into negative end of gel tank and see if it resolves?
Are you adding EtBr to the gel or post-staining? And is it fresh buffer?
February 5, 2026 at 1:10 PM
Are you adding EtBr to the gel or post-staining? And is it fresh buffer?
Badly! Is this just MRC still, had heard rumours BBSRC might be doing same too?
January 30, 2026 at 9:45 AM
Badly! Is this just MRC still, had heard rumours BBSRC might be doing same too?
have you come across Pluronic F-127 - people use for bacterial plates - liquid at 4C but jelly like solid at room temp! pmc.ncbi.nlm.nih.gov/articles/PMC...
An Alternative Gelling Agent for Culture and Studies of Nematodes, Bacteria, Fungi, and Plant Tissues
Pluronic F127 polyol, a block copolymer of propylene oxide and ethylene oxide, was studied as an alternative to agar in culture media for nematodes, bacteria, fungi, actinomycetes, and plant tissues or seedlings, At a polyol concentration of 20% ...
pmc.ncbi.nlm.nih.gov
December 15, 2025 at 2:39 PM
have you come across Pluronic F-127 - people use for bacterial plates - liquid at 4C but jelly like solid at room temp! pmc.ncbi.nlm.nih.gov/articles/PMC...
do preprints count on annual evaulation?
December 12, 2025 at 4:39 PM
do preprints count on annual evaulation?