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Reposted by Boël Lab

Zeynep Baharoglu @zeynepbaharoglu.bsky.social · Sep 6

So excited our antibiotic potentiation story is out 🤩 Led by the extraordinary @manonlang.bsky.social with @fox-science.bsky.social & @amazeld.bsky.social +amazing collaborators @immunobladder.bsky.social @imaneelmeouche.bsky.social 🦠 We believe it can make a difference in #AMR infections!

Uridine as a potentiator of aminoglycosides through activation of carbohydrate transporters

Uridine boosts aminoglycoside treatment efficiency against antibiotic-susceptible as well as antibiotic-resistant E. coli strains.

www.science.org

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Boël Lab @boellab.bsky.social · Sep 5

Congratulations great story

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Boël Lab @boellab.bsky.social · Mar 24

A useful toolkit 🧰⚙️

Maxence Lejars @mlejars.bsky.social · Mar 21

Happy to share our genetic toolkit designed to make your gene editing (life) easier, faster and very efficient!

Also suitable to build saturated chromosomal variant libraries using oligo-recombineering with extra short homology arms!

www.biorxiv.org/content/10.1...

Boël Lab @boellab.bsky.social · Mar 7

Thanks for the great meeting.
It was so nice to see everyone and enjoy great science. 🔤❤️

ABC2025 @abcmeeting.bsky.social · Mar 7

We had an amazing time with the ABC family and already miss you all! Can’t wait to hear your exciting talks, have great discussions, and hit the slopes of the Alps again. #ABC2025

See you next time!🗻⛷️🚠🧬🧫🧪💙

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Reposted by Boël Lab

Mark Peifer (He, him) @peiferlabunc.bsky.social · Mar 1

In a remarkable new paper, Qureshi and Duss use multi-colour single-molecule fluorescence microscopy to directly and simultaneously track transcription, translation & coupling 3/n
www.nature.com/articles/s41...

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Boël Lab @boellab.bsky.social · Mar 3

The second method does the same but also
optimizes all other codons using the 44 best codons identified in our
previously published efficiency codon metric (doi.org/10.1038/natu...). The last method (AG29G) follows a similar
approach but utilizes only the 29 best codons.

Codon influence on protein expression in E. coli correlates with mRNA levels - Nature

In-depth analyses of protein expression studies are used to derive a new codon-influence metric that correlates with global protein levels, mRNA levels and mRNA lifetimes in vivo, indicating tight cou...

doi.org

Boël Lab @boellab.bsky.social · Mar 3

The first
optimization method (AGhead) focuses solely on the first codons to increase the
ratio of adenosine to guanosine.

Codon influence on protein expression in E. coli correlates with mRNA levels - Nature

In-depth analyses of protein expression studies are used to derive a new codon-influence metric that correlates with global protein levels, mRNA levels and mRNA lifetimes in vivo, indicating tight cou...

doi.org

Boël Lab @boellab.bsky.social · Mar 3

The manuscript comes with a web server, opt.williams.edu, that allows for the prediction of expression for
a given DNA sequence and proposes three types of optimization.

OPT codon optimization

opt.williams.edu

Boël Lab @boellab.bsky.social · Mar 3

I am very happy to present our new manuscript in collaboration with the Aalbet and Hunt Labs on DNA sequence optimization for high expression in Escherichia coli as well as in in vitro translation assays (doi.org/10.1016/j.jm...)

Redirecting

doi.org

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Boël Lab @boellab.bsky.social · Mar 1

Super happy to be with the ABC family

ABC2025 @abcmeeting.bsky.social · Mar 1

It's ABC protein, NOT transporter! We are an inclusive bunch! ❤️
Gregory Boel introducing the morning speakers! #ABC2025

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Reposted by Boël Lab

ABC2025 @abcmeeting.bsky.social · Mar 1

It's ABC protein, NOT transporter! We are an inclusive bunch! ❤️
Gregory Boel introducing the morning speakers! #ABC2025

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Boël Lab @boellab.bsky.social · Mar 1

We are all the same super family 🥰

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Reposted by Boël Lab

Cameron Thrash @jcamthrash.bsky.social · Jan 12

Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome www.nature.com/articles/s41... #jcampubs

Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome ...

It can be difficult to find rare transcripts when sequencing a transcriptome. Here the authors show adaptive sampling on direct RNA runs to increase the likelihood of finding less frequent ones while ...

www.nature.com

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Reposted by Boël Lab

John Prensner @johnprensner.bsky.social · Jan 13

check it out!
RiboGraph: An interactive visualization system for ribosome profiling data at read length resolution

🔎 microprotein
🧬 + 🖥️

www.biorxiv.org/content/10.1...

RiboGraph: An interactive visualization system for ribosome profiling data at read length resolution

bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

www.biorxiv.org

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Boël Lab @boellab.bsky.social · Jan 3

Thanks to @onamy.bsky.social for the code 🙏

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Reposted by Boël Lab

Joachim Frank @joachim123.bsky.social · Nov 25

www.biorxiv.org/content/10.1...
Time resolution in cryo-EM using a novel PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling.
Just accepted by CELL as a resource article.

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Boël Lab @boellab.bsky.social · Jan 3

Hi everyone,
We are new on Bluesky 😀. We are a group of microbiologists 🧫🔬 interested in mRNA translation mechanisms and regulation.
We wish you a wonderful year in 2024 🥳🍾 and will be back soon with more news.

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Reposted by Boël Lab

bioRxiv Microbiology @biorxiv-microbiol.bsky.social · Oct 18

Global regulation via modulation of ribosome pausing by EttA https://www.biorxiv.org/content/10.1101/2023.10.17.562674v1

Having multiple rounds of translation of the same mRNA is a key feature of the Central Dogma that cr

www.biorxiv.org

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