This is just the beginning and I’m excited to keep pushing this technology forward in the years ahead.

I’m deeply grateful to my wonderful co-authors, collaborators, and my PhD mentor Dr. Ed Boyden for their support throughout this journey.

Here we establish principles for in situ protein sequencing by demonstrating that peptide sequencing and amino acid recognition are possible within expansion microscopy gels. This is a foundational step toward the long-term goal of single-molecule protein mapping in intact biological specimens.