canopele.bsky.social
Constantin Anoyatis
@canopele.bsky.social
25 followers 64 following 2 posts
Posdoctoral researcher, candidate for weirdest E. coli mutant, at the Hugonnet Lab in Paris. From autolysin roles to (p)ppGpp-mediated resistance🧫
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canopele.bsky.social
Constantin Anoyatis @canopele.bsky.social · 18h
Proud of our new study out in @cp-cellreports.bsky.social!
Using E. coli lacking all 8 endopeptidases, we provide direct evidence that peptidoglycan expansion during elongation requires ED-mediated insertion of one glycan strand at a time. This can be performed by MepS, MepM, MepH and PBP7 #microsky
Role of endopeptidases in lateral cell wall expansion in Escherichia coli
Peptidoglycan, the major constituent of bacterial cell walls, is a giant macromolecule made of glycan strands cross-linked by short peptides, which pr…
www.sciencedirect.com
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canopele.bsky.social
Constantin Anoyatis @canopele.bsky.social · 18h
Proud of our new study out in @cp-cellreports.bsky.social!
Using E. coli lacking all 8 endopeptidases, we provide direct evidence that peptidoglycan expansion during elongation requires ED-mediated insertion of one glycan strand at a time. This can be performed by MepS, MepM, MepH and PBP7 #microsky
Role of endopeptidases in lateral cell wall expansion in Escherichia coli
Peptidoglycan, the major constituent of bacterial cell walls, is a giant macromolecule made of glycan strands cross-linked by short peptides, which pr…
www.sciencedirect.com
1 6 5
Reposted by Constantin Anoyatis
evolvedbiofilm.bsky.social
Ákos T Kovács @evolvedbiofilm.bsky.social · Aug 19
(p)ppGpp-dependent activation of gene expression during nutrient limitation

#mBio from Jonathan Dworkin

journals.asm.org/doi/10.1128/...
(p)ppGpp-dependent activation of gene expression during nutrient limitation | mBio
Bacteria often experience nutrient limitation and, in response, attenuate energetically costly metabolic processes like protein synthesis. At the same time, however, they stimulate the expression of a subset of proteins that facilitate survival under ...
journals.asm.org
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canopele.bsky.social
Constantin Anoyatis @canopele.bsky.social · Jul 29
Congrats @herailquentin.bsky.social
jeh-lab.bsky.social
Hugonnet Lab @jeh-lab.bsky.social · Jul 25
Thrilled to announce our new paper with M. Ethève-Quelquejeu's lab @upcite.bsky.social published @chemistryeurope.bsky.social! We report on the synthesis, and testing by HRMS, of new β-lactam prodrugs, a proof of concept for designing drugs targetting specifically β-lactamase-producing pathogens.
Optimization of Prodrug Activation by Enzymatic Cleavage of the β‐lactam Ring of Carbapenems
We report synthetic routes that yield prodrugs consisting of two carbapenems connected by benzyl carbonate linkers. A mass spectrometry assay was elaborated to monitor, in a single kinetic experiment...
doi.org
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Reposted by Constantin Anoyatis
jeh-lab.bsky.social
Hugonnet Lab @jeh-lab.bsky.social · Jul 25
Excited to present our new study on the mechanism of lateral peptidoglycan expansion in B. subtilis. We combined microscopy visualisation with clickable D-Ala-D-Ala analogues and stable-isotope peptidoglycan labelling to describe an inside-to-outside expansion model @natcomms.nature.com.
Mechanism of lateral cell-wall expansion at a constant diameter in Bacillus subtilis
Nature Communications - In Escherichia coli, lateral cell-wall expansion during growth occurs by crosslinking of new glycan chains to the existing peptidoglycan network. Here, Liang et al. show a...
rdcu.be
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Reposted by Constantin Anoyatis
jeh-lab.bsky.social
Hugonnet Lab @jeh-lab.bsky.social · Jan 21
Very excited to share our work on (p)ppGpp-mediated resistance to beta-lactam antibiotics published in Nature Microbiology!! A great outcome for a project we started working on 5 years ago. A big thank you to all authors that participated in this study!

www.nature.com/articles/s41...
(p)ppGpp modifies RNAP function to confer β-lactam resistance in a peptidoglycan-independent manner - Nature Microbiology
The bacterial alarmone (p)ppGpp induces β-lactam resistance through modification of RNA polymerase and ribosome function rather than regulation of peptidoglycan metabolism in Escherichia coli.
www.nature.com
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