Take-home: ABPP can bridge genotype→phenotype gaps and complement genomic surveillance, a practical way to anticipate cross-resistance and de-risk insecticide rollout/rotation strategies. #malaria #vectorcontrol #chemoproteomics 

Key hit: carboxylesterase Coeae6g. Functional validation shows it confers resistance to pirimiphos-methyl and drives cross-resistance to malathion, bendiocarb, and permethrin

Using fluorophosphonate (FP) probes + pirimiphos-methyl oxon (PMO) competition, we mapped active serine hydrolases in a susceptible Kisumu strain, linking function to candidate resistance drivers (not just expression).