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cellreprolab.bsky.social
Pereira Lab
@cellreprolab.bsky.social
180 followers 360 following 410 posts
Cell Reprogramming in Hematopoiesis and Immunity, Lund University, Sweden. Tweets by Filipe Pereira (signed FP) and Malavika Nair (for Pereira lab). https://pereiralab.com/
pereiralab.com
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · 14d
3/3 By collaborating with Karolinska Institutet, Peng will explore SM-assisted cDC1 reprogramming as a strategy to promote anti-tumor immunity. Way to go, Peng!
cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · 14d
2/3 cDC1 reprogramming offers great immunotherapeutic promise. Yet, viral-mediated transcription factor delivery presents challenges of safety, cost, and scalability. Replacing TFs with SMs can potentially surpass these roadblocks.
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · 14d
1/3 Congrats to our team member @Peng Wei, who was awarded a SciLifeLab PULSE Postdoctoral Fellowship for his project SMARD, which will develop small molecule (SM)-assisted reprogramming to type 1 conventional dendritic cells (cDC1)!
For more info see: pereiralab.com/lab-news/pen...
Peng Wei Awarded a SciLifeLab PULSE Postdoctoral Fellowship - Pereira Lab
The researcher Peng Wei from Pereira Lab was awarded a SciLifeLab PULSE Postdoctoral Fellowship for the project SMARD—Small Molecule-Assisted Cell Reprogramming to cDC1. The SciLifeLab PULSE is a comp...
pereiralab.com
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · 21d
2/2 Yet, dendritic cells had another go with Abigail Altman, who won the Best Poster Award with "Transcription factor blueprints underlying dendritic cell diversity", now out in Immunity (www.cell.com/immunity/ful.... Congrats to both! It's great to have our contributions recognized!
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · 21d
1/2 Celebrating a double win!✨ Yesterday, at the Lund Stem Cell Center (LSCC) retreat, Ervin Ascic was awarded the Article of the Year Award with our Science paper "In vivo dendritic cell reprogramming for cancer immunotherapy" (www.science.org/doi/10.1126/...)
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Sep 8
3/3 If you feel you can contribute, reach out to [email protected] with a possible protocol title and small description.
Let’s connect to push forward a guide for cell engineering and reprogramming strategies! @springernature.com
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Sep 8
2/3 The volume will cover: engineering immune cells with cellular reprogramming; viral and non-viral immune engineering; synthetic biology and genome editing to advance next-generation immune cells; clinical translation of cell engineering and reprogramming-based immunotherapies.
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Sep 8
1/3 Call for Authors!
We are currently editing a volume on Cellular Reprogramming for Immunotherapy for the Methods in Molecular Biology series (Springer Nature). This volume will steer the future of the field and is a great way to share your lab’s protocols!
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Sep 5
Huge thanks to Prof. Allison, and congrats to Ervin, who masterfully defended the promise of cell reprogramming for cancer immunotherapy!
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Sep 5
We are proud to announce that Ervin Ascic successfully defended his PhD yesterday – with the special honor of Nobel Laureate James Allison as opponent! ✨
Ervin’s work makes a strong contribution, showing that in vivo dendritic cell reprogramming is paving the way toward the clinic.
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Aug 29
17/17 🙏🏻 A big thank you to all the funding agencies for making this possible: @ERC_Research, @HorizonEU, @Cancerfonden, @Vetenskapsradet, @novonordiskfond, @fct_pt, #NextgenerationEU, @CancerResearch.
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Aug 29
16/17 👥 We are grateful to our collaborators @AsgardThx, @medeea_matei, @FRosa93, @_CristianaPires, @IsabelUlmert, Signe Holst, Sun-Mi Park, Stefano Vergani, @Katha_lahl, @KharasLab, @TheYuanLab. We thank everyone for your contributions!
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Aug 29
15/17 📖 We want to acknowledge the fantastic contribution from the Pereira Lab: Luis Oliveira, Abigail Altman, Ilia Kurochkin, Ervin Ascic, Evelyn Halitzki, Diogo Cabral, Malavika Nair @pedrocunha37.bsky.social, @WCMMLund, @Lunduniversity, @Lund_Stem , LUCC, @CNC_UC and Region Skåne.
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Aug 29
14/17 🏠 Take-home: In this study we mapped the transcription factor toolkit that builds dendritic cell diversity — a blueprint for reprogramming immune cells against cancer. Read more: doi.org/10.1016/j.im...
Redirecting
doi.org
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Aug 29
13/17 🧩 Our results propose that ETS–IRF pairwise interactions constitute the foundation upon which any DC identity may be built and help define the drivers of DC divergence, advancing our understanding of DC specification and heterogeneity.
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Aug 29
12/17 ⚖️ Surviving mice remained tumor-free upon rechallenge, showing durable immune memory. This finding points toward patient-tailored DC subsets as a new immunotherapy avenue.
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Aug 29
11/17 🐭 Importantly, in melanoma (YUMM1.7) and breast cancer (EO771), in vivo reprogramming towards different iDC subsets drove orthogonal anti-tumor responses, mirroring the natural DC responsiveness on these tumors.
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Aug 29
10/17 💉 In B16 melanoma tumor models, adoptive transfer of iDCs slowed growth and improved survival through distinct mechanisms: iDC2s boosted CD8+ and CD4+ T cells; iLDCs promoted myeloid infiltration.
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Aug 29
9/17 ⚙️ Mechanistically, each TF triad had different chromatin engagement modes and co-bound subset-specific genes early on, showing that DC subset divergence is set in motion at the very start of reprogramming.
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Aug 29
8/17 ⚔️ Functionally, iDC2s resembled inflammatory cDC2Bs with features of interferon-stimulated cDC2s, activating CD8+ T cells via antigen uptake, processing, and cross-presentation, demonstrating real immune potential.
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Aug 29
7/17 🧪 iLDCs expressed an immature pDC gene signature but with a distinct, pro-inflammatory profile. Adding IRF4 to SII boosted IFN-β secretion nearly 5-fold, showing potential for interferon secretion.
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Aug 29
6/17 🎯 iDCs showed subset-specific phenotypes (CLEC12A & SIRPα for PIP; LY6C & B220 for SII) without macrophage or lymphoid markers. PIP-induced cells (iDC2s) shared key cDC2 genes, and SII-induced cells (iLDCs) expressed many pDC genes but lacked some hallmarks (Siglech, Ly6D).
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Aug 29
5/17 🔀 Both combinations rely on an ETS + IRF pair (PU.1/IRF4 or SPIB/IRF8) to prime DC identity. The third factor (PRDM1 or IKZF2) directs the subtype-specific program in induced dendritic cells (iDCs).
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Aug 29
4/17 Screening in mouse embryonic fibroblasts revealed 2 key combos: PIP (PU.1, IRF4, PRDM1) activated Clec9a reporter & CD11b expression showing cDC2-like cell programming; SII (SPIB, IRF8, IKZF2) activated the Clec9a & hCD2 lymphoid reporters & BST2 expression- pDC-like cell
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cellreprolab.bsky.social
Pereira Lab @cellreprolab.bsky.social · Aug 29
3/17 ⚓ We anchored the screen on known DC factors: PU.1, IRF8, and BATF3 induce cDC1s. When comparing their expression across subsets, PU.1 was enriched in cDC2s and IRF8 in pDCs, making them ideal starting points for discovering new “recipes”.
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