Chaney Kalinich
@chaneykalinich.bsky.social
Med student @Yaleschoolofmed and researcher @YaleEMD | into public health, pathogen genomics, field hockey, rock climbing, and Wikipedia rabbit holes
Reposted by Chaney Kalinich
Tiled Amplicon Sequencing Enables Culture-free Whole-Genome Sequencing of Pathogenic Bacteria From Clinical Specimens https://www.biorxiv.org/content/10.1101/2024.12.19.629550v1
December 20, 2024 at 7:35 PM
Tiled Amplicon Sequencing Enables Culture-free Whole-Genome Sequencing of Pathogenic Bacteria From Clinical Specimens https://www.biorxiv.org/content/10.1101/2024.12.19.629550v1
Finally got on Bluesky to share some work I'm really proud of...
Using a tiled amplicon approach, we were able to sequence TB directly from sputum, without culture. This could help make WGS of TB (which can take weeks to culture) much faster at a similar price point to widespread SC2 sequencing.
Using a tiled amplicon approach, we were able to sequence TB directly from sputum, without culture. This could help make WGS of TB (which can take weeks to culture) much faster at a similar price point to widespread SC2 sequencing.
Sharing our work expanding SARS-CoV-2 style tiled amplicon sequencing for whole bacteria genomes directly from clinical samples, showing examples with M.tb (>4 mb) and Strep pneumo (>2 mb).
Led by Chaney Kalinich, Freddy Gonzalez, & Seth Redmond
www.biorxiv.org/content/10.1...
Led by Chaney Kalinich, Freddy Gonzalez, & Seth Redmond
www.biorxiv.org/content/10.1...
December 21, 2024 at 12:50 AM
Finally got on Bluesky to share some work I'm really proud of...
Using a tiled amplicon approach, we were able to sequence TB directly from sputum, without culture. This could help make WGS of TB (which can take weeks to culture) much faster at a similar price point to widespread SC2 sequencing.
Using a tiled amplicon approach, we were able to sequence TB directly from sputum, without culture. This could help make WGS of TB (which can take weeks to culture) much faster at a similar price point to widespread SC2 sequencing.