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Colibri Cytometry

@colibricytometry.bsky.social

20 followers 35 following 5 posts

Tips & tricks for flow cytometry @labliston.bsky.social More on the blog here: https://www.colibri-cytometry.com/blog Also on Substack: https://substack.com/@olivertburton

www.colibri-cytometry.com

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Colibri Cytometry @colibricytometry.bsky.social · May 13

Here's why I do not recommend running new samples using the same unmixing matrix across multiple days. Read more on www.colibri-cytometry.com/post/stabili...

Colibri Cytometry @colibricytometry.bsky.social · Apr 28

Tips today on how to achieve stable unmixing over time by using spectral reference libraries.
www.colibri-cytometry.com/post/stabili...

Stabilizing your unmixing

Today's post pulls from a lot of previous posts on this blog to show how to effectively eliminate batch effects in your flow from an experimental side. Stabilizing the unmixing is a big part of this.

www.colibri-cytometry.com

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Colibri Cytometry @colibricytometry.bsky.social · Apr 14

Do you use phenograph to analyze your cytometry data? Read today's post to learn how to avoid potential pitfalls, particularly if you're using either of the implementations in R.
www.colibri-cytometry.com/post/the-pec...

The peculiarities of Phenograph

Phenograph is one of the more popular clustering algorithms for flow cytometry.

www.colibri-cytometry.com

Colibri Cytometry @colibricytometry.bsky.social · Mar 31

Today's tip: when running your single-color controls, take it slow. The tortoise, as it turns out, beats the hare for spectral flow cytometry.
www.colibri-cytometry.com/post/unmixin...

Unmixing: the tortoise beats the hare

Today's tip: when running your single-color controls, take it slow.

www.colibri-cytometry.com

Colibri Cytometry @colibricytometry.bsky.social · Mar 29

Today's post is very simple--we're just looking at how to create reusable settings on the Cytek Aurora.
www.colibri-cytometry.com/post/creatin...

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