that being said i think it’s very rarely necessary.

caveat that all my experience is with magnetic dynabeads not sepharose.

pre-clearing lysates help a lot when your lysates are particularly sticky (if your medium is dense or there or in nuclear fractions with chromatin present). tradeoff is that your are depleting your sample but hopefully mostly of non-specific interactions.

It’s fortunate that preprint servers are already well-established and robust. As an early career researcher, I still find the pull to publish as prestigiously as possible for my first paper. Recently I’ve started the painful process of disabusing myself of this notion.

With recent NIH news it seems much more plausible that the demise of Journals as the de facto method of sharing science is eminent.

Fascinating study with absolutely mind-blowing good EM images. I’m curious about these trimmer and junction points that form in the HFs. How stable are these structures? Is there a unique lipid composition required for this?

Also, is the idea that this is limited to HF structures or canon MVBs too?

once admirable? our government has always existed to promote the interests of american corporations abroad, at any cost