This study develops a novel 3D human skeletal muscle organoid platform to study the immediate molecular effects of exercise-like contractions. The model uncovers rapid proteomic and transcriptomic re....

Schwann cells (SC) are crucial for physiological impulse conduction in peripheral nerves. They produce myelin, provide axonal metabolic support, and contribute to reparatory processes after nerve inju...

Accurate and reproducible tools to evaluate skeletal muscle contractility help to advance the field of physiology by defining skeletal muscle function in the context of skeletal muscle development, di...

Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene resulting in dystrophin deficiency in skeletal/cardiac muscle and progressive loss of function. While the genetic causes of DMD...

Gravity, the force that structures the cosmos, also shapes human physiology. It influences skeletal, muscular, cardiovascular, respiratory, and neurological systems, sustaining balance, blood circulat...

Background Peripheral nerve injury often leads to muscle atrophy and compromised functional recovery. Growing evidence underscores the critical role of microRNAs (miRNAs) as key epigenetic regulators in this degenerative process. However, most current studies are limited to isolated time points, leaving a gap in the systematic understanding of the temporal dynamics of miRNA expression and their regulatory networks during the initiation of muscle atrophy. Methods and results In this study, we employed small RNA sequencing to dynamically profile miRNA expression in the tibialis anterior muscle across a time series from 12 h to 28 days following denervation. Through differential expression analysis, functional enrichment, and construction of miRNA–mRNA interaction networks integrated with multi-dimensional bioinformatics approaches including GO and KEGG analyses, we identified 199 temporally differentially expressed miRNAs. A pronounced shift in miRNA expression was observed at day 3 post-injury. Functional annotation of target genes revealed an transition in regulatory emphasis from cytoskeletal remodeling toward energy metabolic imbalance. Within this core window of day 3, rno-miR-128-1-5p was found to regulate mitochondrial metabolism-related genes, and cooperated with rno-miR-296-3p in modulating tight junction pathways. Conclusions Our findings illustrate that a time-specific molecular regulatory network underpins the pathological progression of denervation-induced muscle atrophy. These insights offer novel targets for developing precise therapeutic strategies aligned with disease timeline.

Skeletal muscles undergo atrophy in response to denervation and neuromuscular disease. Understanding the mechanisms by which denervation drives muscle atrophy is crucial for developing therapies again...

Background Duchenne muscular dystrophy (DMD) is a lethal pediatric degenerative muscle disease for which there is no cure. Robust preclinical models that recapitulate major clinical features of DMD ar...

Cachexia is a debilitating syndrome marked by progressive skeletal muscle wasting, commonly affecting cancer patients, particularly those with pancreatic cancer. Despite its clinical significance, the...

The role of the nuclear envelope (NE) in skeletal muscle function was first recognized 30 years ago when mutations in NE-associated genes, such as SUN-1, Syne1, Syne2, and LMNA, were linked to muscular dystrophies, also known as nuclear envelopathies. These...

Using a zebrafish-based screen, Civiletto and colleagues identify the herb-derived metabolites thymol and carvacrol as activators of autophagy and mitophagy, demonstrating their therapeutic potential in C. elegans and mouse models.

Khetarpal et al. show that the metabolic regulator PGC-1α is essential in heart muscle cells for exercise-driven cardiac growth, and that suppression of the stress-induced myokine GDF15 is required to enable cardiomyocyte adaptations to training.

CX3CL1 plays a significant role in modulating the skeletal muscle microenvironment that influences properties of both individual fibres and non-muscle cells such as satellite cells, immune cells, and...

Inhibition of MMP9/13 restores both muSC behaviour and regeneration, while macrophage ablation enhances muSC recruitment to the injury, but fails to improve muscle repair in telomerase-deficient zebr...

Ubiquitin is a conserved modifier regulating the stability and function of numerous target proteins. In all eukaryotes, polyubiquitin precursors are g…

The endomysium, the innermost layer of the extracellular matrix (ECM), was shown to play an important role in both passive and active force transmissi…

The development and maintenance of the neuromuscular junction (NMJ) requires reciprocal signals between the nerve terminals and multinucleated skel...

Abstract figure legend Four weeks of semaglutide treatment causes significant reductions in total body weight, fat mass, lean mass, and grip strength in mice with diet induced obesity. Mice treated w...

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

Muscle atrophy is observed in several pathophysiological situations, including physical inactivity, leading to negative health consequences, without a…

Lysosomes are membrane-bound organelles responsible for the degradation of damaged or dysfunctional cellular components, including mitochondria. Their acidic internal environment and the presence of a...

The SMYD family comprises a distinct class of lysine methyltransferases (KMTases) that methylate both histone and non-histone proteins. Among its five members (SMYD1–5), SMYD1 has been identified as a...