Doulin Shepherd
@dcshepherd.bsky.social
PhD student in the Ghosal Lab at the University of Melbourne
I study host pathogen interactions using Cryo-ET and STA stuff
#teamtomo
I study host pathogen interactions using Cryo-ET and STA stuff
#teamtomo
I noticed something similar, especially with membrane proteins. Dynamo worked fine, but I always got strange reconstructions with RELION.
December 12, 2025 at 11:08 AM
I noticed something similar, especially with membrane proteins. Dynamo worked fine, but I always got strange reconstructions with RELION.
I have been using CryoLithe recently as DeepDeWedge and cryoCARE erase many of the periplasmic complexes that I want to pick.
arxiv.org/abs/2501.15246
If you are unfortunate and have to manually pick particles. I have had the most success using the WARP deconvolution filter.
arxiv.org/abs/2501.15246
If you are unfortunate and have to manually pick particles. I have had the most success using the WARP deconvolution filter.
End-to-end localized deep learning for Cryo-ET
Cryo-electron tomography (cryo-ET) enables 3D visualization of cellular environments. Accurate reconstruction of high-resolution volumes is complicated by the very low signal-to-noise ratio and a rest...
arxiv.org
July 11, 2025 at 12:01 AM
I have been using CryoLithe recently as DeepDeWedge and cryoCARE erase many of the periplasmic complexes that I want to pick.
arxiv.org/abs/2501.15246
If you are unfortunate and have to manually pick particles. I have had the most success using the WARP deconvolution filter.
arxiv.org/abs/2501.15246
If you are unfortunate and have to manually pick particles. I have had the most success using the WARP deconvolution filter.