Erman Karasu
@ermankarasu.bsky.social
Research Assistant Professor @Vanderbilt Uni, Lab of Prof. David Cortez
Reposted by Erman Karasu
🥳Just published @nature.com. Over 25 years after the discovery of SPO11 as the enzyme responsible for initiating meiotic recombination, we finally succeeded in reconstituting its DNA cleavage activity.🧵
www.nature.com/articles/s41...
www.nature.com/articles/s41...
SPO11 dimers are sufficient to catalyse DNA double-strand breaks in vitro - Nature
A biochemical system recapitulates the hallmarks of meiotic double-strand break formation, with mouse SPO11 catalysing break formation in the absence of any partners and remaining covalently atta...
www.nature.com
February 19, 2025 at 4:52 PM
🥳Just published @nature.com. Over 25 years after the discovery of SPO11 as the enzyme responsible for initiating meiotic recombination, we finally succeeded in reconstituting its DNA cleavage activity.🧵
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Erman Karasu
Very cool study about selective protein degradation by FBXO31!
🎉Super excited to share our story on how the substrate receptor FBXO31 functions as a quality control factor by recognizing amides. This has been an amazing collaboration between Bode lab and @jcornlab.bsky.social. Special shutout goes to @matthiasmuhar.bsky.social www.nature.com/articles/s41...
C-terminal amides mark proteins for degradation via SCF–FBXO31 - Nature
SCF–FBXO31 scans proteins for C-terminal amidation and marks them for subsequent proteasomal degradation.
www.nature.com
January 29, 2025 at 5:53 PM
Very cool study about selective protein degradation by FBXO31!
Reposted by Erman Karasu
Hello Bluesky, for my first post here I'm happy to share our latest preprint reporting the first biochemical reconstitution of DNA double-strand break formation by SPO11.
SPO11 dimerization controls meiotic DNA double-strand break formation.
www.biorxiv.org/content/10.1...
SPO11 dimerization controls meiotic DNA double-strand break formation.
www.biorxiv.org/content/10.1...
SPO11 dimerization controls meiotic DNA double-strand break formation
SPO11 initiates meiotic recombination through the induction of programmed DNA double-strand breaks (DSBs), but this catalytic activity had never been reconstituted in vitro. Here, using Mus musculus S...
www.biorxiv.org
November 21, 2024 at 10:18 AM
Hello Bluesky, for my first post here I'm happy to share our latest preprint reporting the first biochemical reconstitution of DNA double-strand break formation by SPO11.
SPO11 dimerization controls meiotic DNA double-strand break formation.
www.biorxiv.org/content/10.1...
SPO11 dimerization controls meiotic DNA double-strand break formation.
www.biorxiv.org/content/10.1...
Reposted by Erman Karasu
Hi Bluesky. For my first post, I'd like to advertise the latest preprint from my lab, describing at long last our reconstitution of SPO11-dependent double-strand break formation from purified recombinant proteins. Outstanding work from PhD student Zhi (Zack) Zheng.
November 23, 2024 at 4:00 PM
Hi Bluesky. For my first post, I'd like to advertise the latest preprint from my lab, describing at long last our reconstitution of SPO11-dependent double-strand break formation from purified recombinant proteins. Outstanding work from PhD student Zhi (Zack) Zheng.