@gliperis.bsky.social
🔥 Thank you everyone for attending the #ESHREjc and the first one hosted on Bluesky
Many thanks to our experts for the insightful discussion on the multifaceted role of the oocyte cortex @rvassena.bsky.social @paolavigano.bsky.social
And the # ESHREjc team 💫
Until next time! 👋🏻
Many thanks to our experts for the insightful discussion on the multifaceted role of the oocyte cortex @rvassena.bsky.social @paolavigano.bsky.social
And the # ESHREjc team 💫
Until next time! 👋🏻
Many thanks to all participants, experts, authors and to @gliperis.bsky.social and his team for leading this great first Bluesky edition of the #ESHREjc 🦋
See you next time! 🙌
See you next time! 🙌
January 31, 2026 at 4:05 PM
🔥 Thank you everyone for attending the #ESHREjc and the first one hosted on Bluesky
Many thanks to our experts for the insightful discussion on the multifaceted role of the oocyte cortex @rvassena.bsky.social @paolavigano.bsky.social
And the # ESHREjc team 💫
Until next time! 👋🏻
Many thanks to our experts for the insightful discussion on the multifaceted role of the oocyte cortex @rvassena.bsky.social @paolavigano.bsky.social
And the # ESHREjc team 💫
Until next time! 👋🏻
Question 3 summary of usable clinical markers from the discussion 👇🏻 #ESHREjc
Here the summary of our discussion for Q3 on biomechanic parameters of oocyte developmental competence #ESHREjc
@eshre.bsky.social
@eshre.bsky.social
January 31, 2026 at 3:36 PM
Question 3 summary of usable clinical markers from the discussion 👇🏻 #ESHREjc
Reposted
Here the summary of our discussion for Q3 on biomechanic parameters of oocyte developmental competence #ESHREjc
@eshre.bsky.social
@eshre.bsky.social
January 31, 2026 at 3:26 PM
Here the summary of our discussion for Q3 on biomechanic parameters of oocyte developmental competence #ESHREjc
@eshre.bsky.social
@eshre.bsky.social
What have we been discussing during the #ESHREjc discussion on the oocyte cortex? Have a look at the summaries starting with q1 on its role in maturation 👇🏻
Here’s the summary for Q1 on structural components of the oocyte cortex and GV to MII remodeling #ESHREJC @eshre.bsky.social
January 31, 2026 at 3:04 PM
What have we been discussing during the #ESHREjc discussion on the oocyte cortex? Have a look at the summaries starting with q1 on its role in maturation 👇🏻
Follicular markers can be indicative but face practical difficulties in clinical settings 👇🏻 #ESHREjc
Also technically challenging to keep follicular fluid + cumulus completely separate for each oocyte and send them in for hopefully clinically useful info, for something that we can test with simpler methods (test cortical tension for each oocyte using pipettes) #ESHREjc
January 31, 2026 at 12:23 PM
Follicular markers can be indicative but face practical difficulties in clinical settings 👇🏻 #ESHREjc
Could we find ways to predict oocyte survival post egg freezing cycles? This would be a game changer in counselling for patients undergoing egg freezing for fertility preservation and social reasons #ESHREjc
Exactly, we get rid of cumulus cells to either do ICSI or freeze the oocytes. With ICSI we notice if the cortical tension is off. For freezing we don’t - maybe this could tell us more about oocyte potential if we tested it before freezing? But vitrification probs changes cortex too… #ESHREjc
January 31, 2026 at 12:21 PM
Could we find ways to predict oocyte survival post egg freezing cycles? This would be a game changer in counselling for patients undergoing egg freezing for fertility preservation and social reasons #ESHREjc
How does the follicular environment might affect 👇🏻#ESHREjc
This is an excellent point. We now know that ovarian stiffness (in mice) is correlated with follicular biology (see works by @farnersamargant.bsky.social lab, for instance), I wonder whether the follicular environment effect on oocyte also includes the pressure of its fluid. #ESHREjc
January 31, 2026 at 6:02 AM
How does the follicular environment might affect 👇🏻#ESHREjc
Reposted
On this, I am team pipette. It's not invasive, positioning by the embryologist and automated measure (think of the automated blood pressure band on the arm). The question is, as always, one of sensitivity and sensibility, still very much to be tested.
#ESHREjc
#ESHREjc
January 31, 2026 at 2:12 AM
On this, I am team pipette. It's not invasive, positioning by the embryologist and automated measure (think of the automated blood pressure band on the arm). The question is, as always, one of sensitivity and sensibility, still very much to be tested.
#ESHREjc
#ESHREjc
Reposted
In the fruit fly embryos, mechanical forces modulate the expression of some genes 😎 we would need to look in humans. Maybe blastoids could help?! #ESHREjc
www.cell.com/fulltext/S15... Tissue Deformation Modulates Twist Expression to Determine Anterior Midgut Differentiation in Drosophila Embryos
www.cell.com/fulltext/S15... Tissue Deformation Modulates Twist Expression to Determine Anterior Midgut Differentiation in Drosophila Embryos
Tissue Deformation Modulates Twist Expression to Determine Anterior Midgut Differentiation in Drosophila Embryos
Mechanical deformations associated with embryonic morphogenetic movements have been
suggested to actively participate in the signaling cascades regulating developmental
gene expression. Here we develo...
www.cell.com
January 30, 2026 at 5:32 PM
In the fruit fly embryos, mechanical forces modulate the expression of some genes 😎 we would need to look in humans. Maybe blastoids could help?! #ESHREjc
www.cell.com/fulltext/S15... Tissue Deformation Modulates Twist Expression to Determine Anterior Midgut Differentiation in Drosophila Embryos
www.cell.com/fulltext/S15... Tissue Deformation Modulates Twist Expression to Determine Anterior Midgut Differentiation in Drosophila Embryos
Reposted
Impressive that this paper on characterization of the injection funnel during ICSI has 10 years....new findings can revive older evidence.#ESHREjc
www.sciencedirect.com
January 30, 2026 at 5:41 PM
Impressive that this paper on characterization of the injection funnel during ICSI has 10 years....new findings can revive older evidence.#ESHREjc
Reposted
At the moment, data available finds a difference in biomechanical parameters that can lead to embryo viability and live birth. But the cause of this abnormal parameters will be difficult to adress #ESHREjc
January 30, 2026 at 5:04 PM
At the moment, data available finds a difference in biomechanical parameters that can lead to embryo viability and live birth. But the cause of this abnormal parameters will be difficult to adress #ESHREjc
Question 4 on cortex effects on paracrine interactions #ESHREjc 👇🏻
Q4:Given that the cortex is a part of the interface between the oocyte and cumulus cells, how might abnormalities in the follicular environment alter cortical signaling or architecture, and how could these changes be detected through non-invasive follicular fluid or cumulus cell biomarkers?
#ESHREjc
#ESHREjc
a card that says have an egg-stra egg-cellent day with a blue penguin
ALT: a card that says have an egg-stra egg-cellent day with a blue penguin
media.tenor.com
January 30, 2026 at 5:05 PM
Question 4 on cortex effects on paracrine interactions #ESHREjc 👇🏻
Reposted
2/2
Major barriers to clinical 🧑🏻⚕️ translation remain throughput, operator dependency, lack of standardization, and crucially proving added predictive value beyond existing morphological and morphokinetic markers.
🚨🚨🚨
#ESHREjc
Major barriers to clinical 🧑🏻⚕️ translation remain throughput, operator dependency, lack of standardization, and crucially proving added predictive value beyond existing morphological and morphokinetic markers.
🚨🚨🚨
#ESHREjc
January 30, 2026 at 5:00 PM
2/2
Major barriers to clinical 🧑🏻⚕️ translation remain throughput, operator dependency, lack of standardization, and crucially proving added predictive value beyond existing morphological and morphokinetic markers.
🚨🚨🚨
#ESHREjc
Major barriers to clinical 🧑🏻⚕️ translation remain throughput, operator dependency, lack of standardization, and crucially proving added predictive value beyond existing morphological and morphokinetic markers.
🚨🚨🚨
#ESHREjc
Reposted
Building on Coticchio et al., cortical mechanics as biomarkers is not entirely speculative. For example, Yanez et al. (PNAS, 2016) showed that oocyte stiffness measured by AFM correlates with meiotic competence and spindle integrity.
#ESHREjc
#ESHREjc
January 30, 2026 at 4:55 PM
Building on Coticchio et al., cortical mechanics as biomarkers is not entirely speculative. For example, Yanez et al. (PNAS, 2016) showed that oocyte stiffness measured by AFM correlates with meiotic competence and spindle integrity.
#ESHREjc
#ESHREjc
Question 3 on clinical markers of cortical activity is now live. Join the #ESHREjc discussion 👇🏻
Q3. Coticchio et al suggest cortical properties (stiffness, viscoelasticity) could be biomarkers of oocyte developmental competence. Which technologies 🧫🔬🤖could realistically be applied in an IVF lab 👩🏻🔬 to measure cortex mechanics and what are the major barriers to clinical 🧑🏻⚕️ translation? #ESHREjc
a circle with a blue wave in it
ALT: a circle with a blue wave in it
media.tenor.com
January 30, 2026 at 4:53 PM
Question 3 on clinical markers of cortical activity is now live. Join the #ESHREjc discussion 👇🏻
Data from emerging optical microscopy approaches needed 📣 #ESHREjc
Mouse gives mechanistic resolution, but human validation is essential given cortical thickness/stiffness differences. Key question is whether the logic (polarity modules + spatially distinct myosin pools) is conserved, even if parameters shift. Any human time-lapse/biomech data? #eshrejc
January 30, 2026 at 4:46 PM
Data from emerging optical microscopy approaches needed 📣 #ESHREjc
Multilayered control 🪖⛑️🧢👇🏻 #ESHREjc
Yes, distinct phenotypes make sense cuz Arp2/3 and myosin-II sit in different control layers. Arp2/3 shapes cortical architecture + actin flows that establish/maintain polarity and cap organization, while myosin-II executes stage-specific force generation needed for rotation/cytokinesis. #eshrejc
January 30, 2026 at 4:43 PM
Multilayered control 🪖⛑️🧢👇🏻 #ESHREjc
Reposted
Fyn-null oocytes have problems in maintaining cortical integrity....they are subfertile #ESHREjc
Fyn kinase is especially interesting as a signalling integrator. It links chromatin proximity to local cortical remodelling. How direct is the chromatin to cortex signalling axis and are there specific downstream actin regulators involved? #ESHREjc
January 30, 2026 at 4:27 PM
Fyn-null oocytes have problems in maintaining cortical integrity....they are subfertile #ESHREjc
Reposted
Studying ectopic chromosomal signaling that misactivates RhoA–myosin II at the cortex is fundamental to understanding this key mechanism underlying abnormal cleavage and fragmentation. #ESHREjc
a man looking through a doorway at a framed picture of a game show called the price is right
ALT: a man looking through a doorway at a framed picture of a game show called the price is right
media.tenor.com
January 30, 2026 at 4:31 PM
Studying ectopic chromosomal signaling that misactivates RhoA–myosin II at the cortex is fundamental to understanding this key mechanism underlying abnormal cleavage and fragmentation. #ESHREjc
The effects of cortical dysregulation in embryo development are discussed in the 2nd question of the #ESHREjc 👇🏻
Q2: The paper proposed that cortical dysregulation can lead to blastomeri fragmentation and reduced blastocyst quality. What are the proposed mechanisms by which ectopic chromosomal signaling or cortical instability leads to abnormal cleavage patterns in early embryos? #ESHREjc
January 30, 2026 at 4:27 PM
The effects of cortical dysregulation in embryo development are discussed in the 2nd question of the #ESHREjc 👇🏻
Reposted
Appropriate stiffness due to acto-myosin network is crucial for splindle positioning and PB extrusion. #ESHREjc
Their role in GV to MII translation is critical with failure to act having the same consequences as your GIF failing to be uploaded here in the question #ESHREjc
January 30, 2026 at 4:20 PM
Appropriate stiffness due to acto-myosin network is crucial for splindle positioning and PB extrusion. #ESHREjc
The #ESHREjc is underway 👇🏻
Q1. How do the structural components of the oocyte cortex (e.g., actin, myosin-!, pERM, Arp2/3, Fyn kinases) dynamically remodel during the transitions from GV to MIl and how do these changes mechanistically support spindle positioning and polar body extrusion? #ESHREjc
a picture of a woman with the words i 'm just an egg
ALT: a picture of a woman with the words i 'm just an egg
media.tenor.com
January 30, 2026 at 4:13 PM
The #ESHREjc is underway 👇🏻
Revisit the #bluetorial for the discussed paper 👇🏻 #ESHREjc
🧬 The Oocyte Cortex: Fertility’s Hidden Control Layer
To find out join #ESHREjc on Friday 29/1 starting 17:00 CET.
@paolavigano.bsky.social @rvassena.bsky.social @enricabianchi.bsky.social&authors Giovanni Cotichio and Danilo Cimadomo
Paper ➡️ pubmed.ncbi.nlm.nih.gov/41206604/
🧵 #bluetorial 👇🏻
To find out join #ESHREjc on Friday 29/1 starting 17:00 CET.
@paolavigano.bsky.social @rvassena.bsky.social @enricabianchi.bsky.social&authors Giovanni Cotichio and Danilo Cimadomo
Paper ➡️ pubmed.ncbi.nlm.nih.gov/41206604/
🧵 #bluetorial 👇🏻
The emerging role of the oocyte cortical domain in maturation, fertilization, and development - PubMed
The cell cortex, a cytoskeletal network with regulatory signalling pathways, is localized beneath the cell membrane: it is especially prominent in mammalian oocytes. As in other cells, the cortex ensures appropriate shape and robustness of oocytes. It is also involved in other key and more specific …
pubmed.ncbi.nlm.nih.gov
January 30, 2026 at 4:08 PM
Revisit the #bluetorial for the discussed paper 👇🏻 #ESHREjc