H. B. Beryl Rappaport
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hbrappap.bsky.social
H. B. Beryl Rappaport
@hbrappap.bsky.social
PhD Student @ Syracuse University
protists, extremophiles, & science art | she/her | 🔬🏳️‍🌈
Thanks Julia!!
December 22, 2025 at 8:55 PM
Yes samples were kept at ambient temps so likely we are missing out on any thermophiles that can’t encyst at lower temps - hoping at some point to sequence samples preserved in the field in part because of this!
December 2, 2025 at 8:45 PM
Awesome, that is good with us :)
December 1, 2025 at 11:06 PM
Yes, that’s the place! Cool, interested in what you’ve found there
December 1, 2025 at 11:05 PM
thanks for these musings!
December 1, 2025 at 7:25 PM
yes we just have multiple filamentous bacteria in the culture, but M. ruber is definitely there and seems to be most abundant based on sequencing!
November 28, 2025 at 7:51 PM
most likely M. ruber! so interesting, didn’t know that about filamentation! 🍝
& not sure why the file conversions were named that way but the order looks accurate as labeled - Supplemental Movie 1-12
November 27, 2025 at 7:23 PM
the long thin guys are filamentous bacteria and the clump is moving oddly but I believe it’s just bacterial biofilm
November 27, 2025 at 4:09 PM
so awesome!
November 27, 2025 at 4:08 PM
Tbd! We need to redo some staining
November 26, 2025 at 11:57 PM
thermophilic bacteria!
November 26, 2025 at 11:51 PM
I believe within their tubes they can withstand higher temps but the worms themselves max out around 55°C, and temps for reproduction and early dev may be lower - their max tolerance is super interesting though! pmc.ncbi.nlm.nih.gov/articles/PMC...
Thermal Limit for Metazoan Life in Question: In Vivo Heat Tolerance of the Pompeii Worm
The thermal limit for metazoan life, expected to be around 50°C, has been debated since the discovery of the Pompeii worm Alvinella pompejana, which colonizes black smoker chimney walls at deep-sea vents. While indirect evidence predicts body ...
pmc.ncbi.nlm.nih.gov
November 26, 2025 at 3:25 AM
We are looking into this 👀
November 26, 2025 at 3:19 AM
We will have all sequences up on NCBI with publication for you to take a look! Also really interested in their chaperone network!
November 25, 2025 at 10:05 PM
And finally all at SyracuseU - @shaharsu.bsky.social @jessniblo.bsky.social for protein analyses, @rmshepherd.bsky.social for culturing, & @oliverio.bsky.social for leading this effort with me!
November 25, 2025 at 8:41 PM
@xtremevirusprof.bsky.social @nachopus.bsky.social and team at PSU and Gordon Wolfe at Chico for sampling and extremophile expertise

@lauraakatz.bsky.social and Godwin Ani at Smith for phylogenomics
November 25, 2025 at 8:41 PM
@fmschu.bsky.social New Lineages of Life group at JGI with Tomas Tyml for culturing, seq prep, & biogeography, and Kurt LaButti also at LBNL for bioinformatics

@fmikus.bsky.social @dudinlab.bsky.social at Geneva and @gautamdey.bsky.social at EMBL for beautiful U-ExM
November 25, 2025 at 8:41 PM
So grateful to all collaborators on this huge effort!
@mullinslab.bsky.social at UCSF with Natalie Petek, @samjlord.bsky.social, and @skruberk.bsky.social now at Gonzaga with Ethan MacVicar for microscopy & motility analyses
November 25, 2025 at 8:41 PM